Helical reconstruction of Salmonella and Shigella needle filaments attached to type 3 basal bodies

Vadim Kotov; Michele Lunelli; Jiri Wald; Michael Kolbe; Thomas C Marlovits

doi:10.1016/j.bbrep.2021.101039

Helical reconstruction of Salmonella and Shigella needle filaments attached to type 3 basal bodies

Standard

Helical reconstruction of Salmonella and Shigella needle filaments attached to type 3 basal bodies. / Kotov, Vadim; Lunelli, Michele; Wald, Jiri; Kolbe, Michael; Marlovits, Thomas C.

In: BIOCHEM BIOPHYS REP, Vol. 27, 09.2021, p. 101039.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Kotov, V, Lunelli, M, Wald, J, Kolbe, M & Marlovits, TC 2021, 'Helical reconstruction of Salmonella and Shigella needle filaments attached to type 3 basal bodies', BIOCHEM BIOPHYS REP, vol. 27, pp. 101039. https://doi.org/10.1016/j.bbrep.2021.101039

APA

Kotov, V., Lunelli, M., Wald, J., Kolbe, M., & Marlovits, T. C. (2021). Helical reconstruction of Salmonella and Shigella needle filaments attached to type 3 basal bodies. BIOCHEM BIOPHYS REP, 27, 101039. https://doi.org/10.1016/j.bbrep.2021.101039

Vancouver

Kotov V, Lunelli M, Wald J, Kolbe M, Marlovits TC. Helical reconstruction of Salmonella and Shigella needle filaments attached to type 3 basal bodies. BIOCHEM BIOPHYS REP. 2021 Sep;27:101039. https://doi.org/10.1016/j.bbrep.2021.101039

Bibtex

@article{13e0f9042b7b4496951e546345f91dbd,

title = "Helical reconstruction of Salmonella and Shigella needle filaments attached to type 3 basal bodies",

abstract = "Gram-negative pathogens evolved a syringe-like nanomachine, termed type 3 secretion system, to deliver protein effectors into the cytoplasm of host cells. An essential component of this system is a long helical needle filament that protrudes from the bacterial surface and connects the cytoplasms of the bacterium and the eukaryotic cell. Previous structural research was predominantly focused on reconstituted type 3 needle filaments, which lacked the biological context. In this work we introduce a facile procedure to obtain high-resolution cryo-EM structure of needle filaments attached to the basal body of type 3 secretion systems. We validate our approach by solving the structure of Salmonella PrgI filament and demonstrate its utility by obtaining the first high-resolution cryo-EM reconstruction of Shigella MxiH filament. Our work paves the way to systematic structural characterization of attached type 3 needle filaments in the context of mutagenesis studies, protein structural evolution and drug development.",

author = "Vadim Kotov and Michele Lunelli and Jiri Wald and Michael Kolbe and Marlovits, {Thomas C}",

note = "{\textcopyright} 2021 Published by Elsevier B.V.",

year = "2021",

month = sep,

doi = "10.1016/j.bbrep.2021.101039",

language = "English",

volume = "27",

pages = "101039",

journal = "BIOCHEM BIOPHYS REP",

issn = "2405-5808",

publisher = "Elsevier BV",

}

RIS

TY - JOUR

T1 - Helical reconstruction of Salmonella and Shigella needle filaments attached to type 3 basal bodies

AU - Kotov, Vadim

AU - Lunelli, Michele

AU - Wald, Jiri

AU - Kolbe, Michael

AU - Marlovits, Thomas C

PY - 2021/9

Y1 - 2021/9

N2 - Gram-negative pathogens evolved a syringe-like nanomachine, termed type 3 secretion system, to deliver protein effectors into the cytoplasm of host cells. An essential component of this system is a long helical needle filament that protrudes from the bacterial surface and connects the cytoplasms of the bacterium and the eukaryotic cell. Previous structural research was predominantly focused on reconstituted type 3 needle filaments, which lacked the biological context. In this work we introduce a facile procedure to obtain high-resolution cryo-EM structure of needle filaments attached to the basal body of type 3 secretion systems. We validate our approach by solving the structure of Salmonella PrgI filament and demonstrate its utility by obtaining the first high-resolution cryo-EM reconstruction of Shigella MxiH filament. Our work paves the way to systematic structural characterization of attached type 3 needle filaments in the context of mutagenesis studies, protein structural evolution and drug development.

AB - Gram-negative pathogens evolved a syringe-like nanomachine, termed type 3 secretion system, to deliver protein effectors into the cytoplasm of host cells. An essential component of this system is a long helical needle filament that protrudes from the bacterial surface and connects the cytoplasms of the bacterium and the eukaryotic cell. Previous structural research was predominantly focused on reconstituted type 3 needle filaments, which lacked the biological context. In this work we introduce a facile procedure to obtain high-resolution cryo-EM structure of needle filaments attached to the basal body of type 3 secretion systems. We validate our approach by solving the structure of Salmonella PrgI filament and demonstrate its utility by obtaining the first high-resolution cryo-EM reconstruction of Shigella MxiH filament. Our work paves the way to systematic structural characterization of attached type 3 needle filaments in the context of mutagenesis studies, protein structural evolution and drug development.

U2 - 10.1016/j.bbrep.2021.101039

DO - 10.1016/j.bbrep.2021.101039

M3 - SCORING: Journal article

C2 - 34258394

VL - 27

SP - 101039

JO - BIOCHEM BIOPHYS REP

JF - BIOCHEM BIOPHYS REP

SN - 2405-5808

ER -