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HCV-specific CD4+ T cells of patients with acute and chronic HCV infection display high expression of TIGIT and other co-inhibitory molecules

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HCV-specific CD4+ T cells of patients with acute and chronic HCV infection display high expression of TIGIT and other co-inhibitory molecules. / Ackermann, Christin; Smits, Maike; Woost, Robin; Eberhard, Johanna M; Peine, Sven; Kummer, Silke; Marget, Matthias; Kuntzen, Thomas; Kwok, William W; Lohse, Ansgar W; Jacobs, Thomas; Boettler, Tobias; Schulze Zur Wiesch, Julian.

In: SCI REP-UK, Vol. 9, No. 1, 23.07.2019, p. 10624.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Ackermann, C, Smits, M, Woost, R, Eberhard, JM, Peine, S, Kummer, S, Marget, M, Kuntzen, T, Kwok, WW, Lohse, AW, Jacobs, T, Boettler, T & Schulze Zur Wiesch, J 2019, 'HCV-specific CD4+ T cells of patients with acute and chronic HCV infection display high expression of TIGIT and other co-inhibitory molecules', SCI REP-UK, vol. 9, no. 1, pp. 10624. https://doi.org/10.1038/s41598-019-47024-8

APA

Ackermann, C., Smits, M., Woost, R., Eberhard, J. M., Peine, S., Kummer, S., Marget, M., Kuntzen, T., Kwok, W. W., Lohse, A. W., Jacobs, T., Boettler, T., & Schulze Zur Wiesch, J. (2019). HCV-specific CD4+ T cells of patients with acute and chronic HCV infection display high expression of TIGIT and other co-inhibitory molecules. SCI REP-UK, 9(1), 10624. https://doi.org/10.1038/s41598-019-47024-8

Vancouver

Ackermann C, Smits M, Woost R, Eberhard JM, Peine S, Kummer S et al. HCV-specific CD4+ T cells of patients with acute and chronic HCV infection display high expression of TIGIT and other co-inhibitory molecules. SCI REP-UK. 2019 Jul 23;9(1):10624. https://doi.org/10.1038/s41598-019-47024-8

Bibtex

@article{4f0d2cd4cda644b58f88b4a62b51c107,
title = "HCV-specific CD4+ T cells of patients with acute and chronic HCV infection display high expression of TIGIT and other co-inhibitory molecules",
abstract = "The combined regulation of a network of inhibitory and activating T cell receptors may be a critical step in the development of chronic HCV infection. Ex vivo HCV MHC class I + II tetramer staining and bead-enrichment was performed with baseline and longitudinal PBMC samples of a cohort of patients with acute, chronic and spontaneously resolved HCV infection to assess the expression pattern of the co-inhibitory molecule TIGIT together with PD-1, BTLA, Tim-3, as well as OX40 and CD226 (DNAM-1) of HCV-specific CD4+ T cells, and in a subset of patients of HCV-specific CD8+ T cells. As the main result, we found a higher expression level of TIGIT+ PD-1+ on HCV-specific CD4+ T cells during acute and chronic HCV infection compared to patients with spontaneously resolved HCV infection (p < 0,0001). Conversely, expression of the complementary co-stimulatory receptor of TIGIT, CD226 (DNAM-1) was significantly decreased on HCV-specific CD4+ T cells during chronic infection. The predominant phenotype of HCV-specific CD4+ T cells during acute and chronic infection was TIGIT+, PD-1+, BTLA+, Tim-3-. This comprehensive phenotypic study confirms TIGIT together with PD-1 as a discriminatory marker of dysfunctional HCV-specific CD4+ T cells.",
author = "Christin Ackermann and Maike Smits and Robin Woost and Eberhard, {Johanna M} and Sven Peine and Silke Kummer and Matthias Marget and Thomas Kuntzen and Kwok, {William W} and Lohse, {Ansgar W} and Thomas Jacobs and Tobias Boettler and {Schulze Zur Wiesch}, Julian",
year = "2019",
month = jul,
day = "23",
doi = "10.1038/s41598-019-47024-8",
language = "English",
volume = "9",
pages = "10624",
journal = "SCI REP-UK",
issn = "2045-2322",
publisher = "NATURE PUBLISHING GROUP",
number = "1",

}

RIS

TY - JOUR

T1 - HCV-specific CD4+ T cells of patients with acute and chronic HCV infection display high expression of TIGIT and other co-inhibitory molecules

AU - Ackermann, Christin

AU - Smits, Maike

AU - Woost, Robin

AU - Eberhard, Johanna M

AU - Peine, Sven

AU - Kummer, Silke

AU - Marget, Matthias

AU - Kuntzen, Thomas

AU - Kwok, William W

AU - Lohse, Ansgar W

AU - Jacobs, Thomas

AU - Boettler, Tobias

AU - Schulze Zur Wiesch, Julian

PY - 2019/7/23

Y1 - 2019/7/23

N2 - The combined regulation of a network of inhibitory and activating T cell receptors may be a critical step in the development of chronic HCV infection. Ex vivo HCV MHC class I + II tetramer staining and bead-enrichment was performed with baseline and longitudinal PBMC samples of a cohort of patients with acute, chronic and spontaneously resolved HCV infection to assess the expression pattern of the co-inhibitory molecule TIGIT together with PD-1, BTLA, Tim-3, as well as OX40 and CD226 (DNAM-1) of HCV-specific CD4+ T cells, and in a subset of patients of HCV-specific CD8+ T cells. As the main result, we found a higher expression level of TIGIT+ PD-1+ on HCV-specific CD4+ T cells during acute and chronic HCV infection compared to patients with spontaneously resolved HCV infection (p < 0,0001). Conversely, expression of the complementary co-stimulatory receptor of TIGIT, CD226 (DNAM-1) was significantly decreased on HCV-specific CD4+ T cells during chronic infection. The predominant phenotype of HCV-specific CD4+ T cells during acute and chronic infection was TIGIT+, PD-1+, BTLA+, Tim-3-. This comprehensive phenotypic study confirms TIGIT together with PD-1 as a discriminatory marker of dysfunctional HCV-specific CD4+ T cells.

AB - The combined regulation of a network of inhibitory and activating T cell receptors may be a critical step in the development of chronic HCV infection. Ex vivo HCV MHC class I + II tetramer staining and bead-enrichment was performed with baseline and longitudinal PBMC samples of a cohort of patients with acute, chronic and spontaneously resolved HCV infection to assess the expression pattern of the co-inhibitory molecule TIGIT together with PD-1, BTLA, Tim-3, as well as OX40 and CD226 (DNAM-1) of HCV-specific CD4+ T cells, and in a subset of patients of HCV-specific CD8+ T cells. As the main result, we found a higher expression level of TIGIT+ PD-1+ on HCV-specific CD4+ T cells during acute and chronic HCV infection compared to patients with spontaneously resolved HCV infection (p < 0,0001). Conversely, expression of the complementary co-stimulatory receptor of TIGIT, CD226 (DNAM-1) was significantly decreased on HCV-specific CD4+ T cells during chronic infection. The predominant phenotype of HCV-specific CD4+ T cells during acute and chronic infection was TIGIT+, PD-1+, BTLA+, Tim-3-. This comprehensive phenotypic study confirms TIGIT together with PD-1 as a discriminatory marker of dysfunctional HCV-specific CD4+ T cells.

U2 - 10.1038/s41598-019-47024-8

DO - 10.1038/s41598-019-47024-8

M3 - SCORING: Journal article

C2 - 31337800

VL - 9

SP - 10624

JO - SCI REP-UK

JF - SCI REP-UK

SN - 2045-2322

IS - 1

ER -