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Gut inflammation and expression of ICC in a fetal lamb model of fetoscopic intervention for gastroschisis

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Gut inflammation and expression of ICC in a fetal lamb model of fetoscopic intervention for gastroschisis. / Krebs, Thomas; Boettcher, Michael; Schäfer, Hansjoerg; Eschenburg, Georg; Wenke, Katharina; Appl, Birgit; Roth, Beate; Andreas, Thomas; Schmitz, Carla; Fahje, Rebecca; Jacobsen, Birte; Tiemann, Bastian; Reinshagen, Konrad; Hecher, Kurt; Bergholz, Robert.

In: SURG ENDOSC, Vol. 28, No. 8, 20.03.2014, p. 2437-2442.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Krebs, T, Boettcher, M, Schäfer, H, Eschenburg, G, Wenke, K, Appl, B, Roth, B, Andreas, T, Schmitz, C, Fahje, R, Jacobsen, B, Tiemann, B, Reinshagen, K, Hecher, K & Bergholz, R 2014, 'Gut inflammation and expression of ICC in a fetal lamb model of fetoscopic intervention for gastroschisis', SURG ENDOSC, vol. 28, no. 8, pp. 2437-2442. https://doi.org/10.1007/s00464-014-3494-x

APA

Krebs, T., Boettcher, M., Schäfer, H., Eschenburg, G., Wenke, K., Appl, B., Roth, B., Andreas, T., Schmitz, C., Fahje, R., Jacobsen, B., Tiemann, B., Reinshagen, K., Hecher, K., & Bergholz, R. (2014). Gut inflammation and expression of ICC in a fetal lamb model of fetoscopic intervention for gastroschisis. SURG ENDOSC, 28(8), 2437-2442. https://doi.org/10.1007/s00464-014-3494-x

Vancouver

Krebs T, Boettcher M, Schäfer H, Eschenburg G, Wenke K, Appl B et al. Gut inflammation and expression of ICC in a fetal lamb model of fetoscopic intervention for gastroschisis. SURG ENDOSC. 2014 Mar 20;28(8):2437-2442. https://doi.org/10.1007/s00464-014-3494-x

Bibtex

@article{e63f11dabd0441b59689006784503b09,
title = "Gut inflammation and expression of ICC in a fetal lamb model of fetoscopic intervention for gastroschisis",
abstract = "BACKGROUND: The pathogenesis of intestinal dysmotility in gastroschisis is not completely understood. Peel formation and disorganization of interstitial Cajal cells (ICC) have been proposed in humans. The aim of this study was to evaluate the impact of prenatal coverage of gastroschisis on gut inflammation and expression of ICC in a fetal lamb model.METHODS: Twenty-one German blackhead sheep with an abdominal wall defect that was created fetoscopically on day 77 of 145 days gestation were used in this study. Intrauterine surgery with the aim to cover the defect was performed 3 weeks later; two fetuses were covered completely, 5 partially and 11 remained uncovered. Three fetuses without gastroschisis were used as controls. All fetuses were retrieved by cesarean section at day 135. Samples of the small intestine were stained with hematoxylin and eosin for histologic analysis of peel formation and serosal and muscular thickness. For ICC detection, immunohistochemistry using anti-CD117 (c-Kit) antibody was used.RESULTS: In all samples with exposure to amniotic fluid, peel formation and significantly decreased ICC were found. Complete coverage reduced peel formation and disorganization of ICC compared to uncovered animals almost to the level of controls.CONCLUSIONS: Peel formation and ICC derangement were significantly reduced by prenatal coverage of gastroschisis. Moreover, this animal model mimics the histopathological bowel changes as seen in human gastroschisis and may, therefore, be used for further research on the pathophysiology and fetal therapy of this malformation.",
author = "Thomas Krebs and Michael Boettcher and Hansjoerg Sch{\"a}fer and Georg Eschenburg and Katharina Wenke and Birgit Appl and Beate Roth and Thomas Andreas and Carla Schmitz and Rebecca Fahje and Birte Jacobsen and Bastian Tiemann and Konrad Reinshagen and Kurt Hecher and Robert Bergholz",
year = "2014",
month = mar,
day = "20",
doi = "10.1007/s00464-014-3494-x",
language = "English",
volume = "28",
pages = "2437--2442",
journal = "SURG ENDOSC",
issn = "0930-2794",
publisher = "Springer New York",
number = "8",

}

RIS

TY - JOUR

T1 - Gut inflammation and expression of ICC in a fetal lamb model of fetoscopic intervention for gastroschisis

AU - Krebs, Thomas

AU - Boettcher, Michael

AU - Schäfer, Hansjoerg

AU - Eschenburg, Georg

AU - Wenke, Katharina

AU - Appl, Birgit

AU - Roth, Beate

AU - Andreas, Thomas

AU - Schmitz, Carla

AU - Fahje, Rebecca

AU - Jacobsen, Birte

AU - Tiemann, Bastian

AU - Reinshagen, Konrad

AU - Hecher, Kurt

AU - Bergholz, Robert

PY - 2014/3/20

Y1 - 2014/3/20

N2 - BACKGROUND: The pathogenesis of intestinal dysmotility in gastroschisis is not completely understood. Peel formation and disorganization of interstitial Cajal cells (ICC) have been proposed in humans. The aim of this study was to evaluate the impact of prenatal coverage of gastroschisis on gut inflammation and expression of ICC in a fetal lamb model.METHODS: Twenty-one German blackhead sheep with an abdominal wall defect that was created fetoscopically on day 77 of 145 days gestation were used in this study. Intrauterine surgery with the aim to cover the defect was performed 3 weeks later; two fetuses were covered completely, 5 partially and 11 remained uncovered. Three fetuses without gastroschisis were used as controls. All fetuses were retrieved by cesarean section at day 135. Samples of the small intestine were stained with hematoxylin and eosin for histologic analysis of peel formation and serosal and muscular thickness. For ICC detection, immunohistochemistry using anti-CD117 (c-Kit) antibody was used.RESULTS: In all samples with exposure to amniotic fluid, peel formation and significantly decreased ICC were found. Complete coverage reduced peel formation and disorganization of ICC compared to uncovered animals almost to the level of controls.CONCLUSIONS: Peel formation and ICC derangement were significantly reduced by prenatal coverage of gastroschisis. Moreover, this animal model mimics the histopathological bowel changes as seen in human gastroschisis and may, therefore, be used for further research on the pathophysiology and fetal therapy of this malformation.

AB - BACKGROUND: The pathogenesis of intestinal dysmotility in gastroschisis is not completely understood. Peel formation and disorganization of interstitial Cajal cells (ICC) have been proposed in humans. The aim of this study was to evaluate the impact of prenatal coverage of gastroschisis on gut inflammation and expression of ICC in a fetal lamb model.METHODS: Twenty-one German blackhead sheep with an abdominal wall defect that was created fetoscopically on day 77 of 145 days gestation were used in this study. Intrauterine surgery with the aim to cover the defect was performed 3 weeks later; two fetuses were covered completely, 5 partially and 11 remained uncovered. Three fetuses without gastroschisis were used as controls. All fetuses were retrieved by cesarean section at day 135. Samples of the small intestine were stained with hematoxylin and eosin for histologic analysis of peel formation and serosal and muscular thickness. For ICC detection, immunohistochemistry using anti-CD117 (c-Kit) antibody was used.RESULTS: In all samples with exposure to amniotic fluid, peel formation and significantly decreased ICC were found. Complete coverage reduced peel formation and disorganization of ICC compared to uncovered animals almost to the level of controls.CONCLUSIONS: Peel formation and ICC derangement were significantly reduced by prenatal coverage of gastroschisis. Moreover, this animal model mimics the histopathological bowel changes as seen in human gastroschisis and may, therefore, be used for further research on the pathophysiology and fetal therapy of this malformation.

U2 - 10.1007/s00464-014-3494-x

DO - 10.1007/s00464-014-3494-x

M3 - SCORING: Journal article

C2 - 24648107

VL - 28

SP - 2437

EP - 2442

JO - SURG ENDOSC

JF - SURG ENDOSC

SN - 0930-2794

IS - 8

ER -