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Gut Dysbiosis With Bacilli Dominance and Accumulation of Fermentation Products Precedes Late-onset Sepsis in Preterm Infants

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Gut Dysbiosis With Bacilli Dominance and Accumulation of Fermentation Products Precedes Late-onset Sepsis in Preterm Infants. / Graspeuntner, S; Waschina, S; Künzel, S; Twisselmann, N; Rausch, T K; Cloppenborg-Schmidt, K; Zimmermann, J; Viemann, D; Herting, E; Göpel, W; Baines, J F; Kaleta, C; Rupp, J; Härtel, C; Pagel, J.

In: CLIN INFECT DIS, Vol. 69, No. 2, 02.07.2019, p. 268-277.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Graspeuntner, S, Waschina, S, Künzel, S, Twisselmann, N, Rausch, TK, Cloppenborg-Schmidt, K, Zimmermann, J, Viemann, D, Herting, E, Göpel, W, Baines, JF, Kaleta, C, Rupp, J, Härtel, C & Pagel, J 2019, 'Gut Dysbiosis With Bacilli Dominance and Accumulation of Fermentation Products Precedes Late-onset Sepsis in Preterm Infants', CLIN INFECT DIS, vol. 69, no. 2, pp. 268-277. https://doi.org/10.1093/cid/ciy882

APA

Graspeuntner, S., Waschina, S., Künzel, S., Twisselmann, N., Rausch, T. K., Cloppenborg-Schmidt, K., Zimmermann, J., Viemann, D., Herting, E., Göpel, W., Baines, J. F., Kaleta, C., Rupp, J., Härtel, C., & Pagel, J. (2019). Gut Dysbiosis With Bacilli Dominance and Accumulation of Fermentation Products Precedes Late-onset Sepsis in Preterm Infants. CLIN INFECT DIS, 69(2), 268-277. https://doi.org/10.1093/cid/ciy882

Vancouver

Graspeuntner S, Waschina S, Künzel S, Twisselmann N, Rausch TK, Cloppenborg-Schmidt K et al. Gut Dysbiosis With Bacilli Dominance and Accumulation of Fermentation Products Precedes Late-onset Sepsis in Preterm Infants. CLIN INFECT DIS. 2019 Jul 2;69(2):268-277. https://doi.org/10.1093/cid/ciy882

Bibtex

@article{56a7e3e2e5c74757a76229a2c806ced9,
title = "Gut Dysbiosis With Bacilli Dominance and Accumulation of Fermentation Products Precedes Late-onset Sepsis in Preterm Infants",
abstract = "BACKGROUND: Gut dysbiosis has been suggested as a major risk factor for the development of late-onset sepsis (LOS), a main cause of mortality and morbidity in preterm infants. We aimed to assess specific signatures of the gut microbiome, including metabolic profiles, in preterm infants <34 weeks of gestation preceding LOS.METHODS: In a single-center cohort, fecal samples from preterm infants were prospectively collected during the period of highest vulnerability for LOS (days 7, 14, and 21 of life). Following 16S rRNA gene profiling, we assessed microbial community function using microbial metabolic network modeling. Data were adjusted for gestational age and use of probiotics.RESULTS: We studied stool samples from 71 preterm infants with LOS and 164 unaffected controls (no LOS/necrotizing enterocolitis). In most cases, the bacteria isolated in diagnostic blood culture corresponded to the genera in the gut microbiome. LOS cases had a decelerated development of microbial diversity. Before onset of disease, LOS cases had specific gut microbiome signatures with higher abundance of Bacilli (specifically coagulase-negative Staphylococci) and a lack of anaerobic bacteria. In silico modeling of bacterial community metabolism suggested accumulation of the fermentation products ethanol and formic acid in LOS cases before the onset of disease.CONCLUSIONS: Intestinal dysbiosis preceding LOS is characterized by an accumulation of Bacilli and their fermentation products and a paucity of anaerobic bacteria. Early microbiome and metabolic patterns may become a valuable biomarker to guide individualized prevention strategies of LOS in highly vulnerable populations.",
keywords = "Anaerobiosis, Cluster Analysis, DNA, Bacterial/chemistry, DNA, Ribosomal/chemistry, Dysbiosis/complications, Feces/chemistry, Gastrointestinal Microbiome, Humans, Infant, Infant, Newborn, Infant, Premature, Male, Metabolome, Metabolomics, Metagenomics, Neonatal Sepsis/pathology, Phylogeny, Prospective Studies, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA",
author = "S Graspeuntner and S Waschina and S K{\"u}nzel and N Twisselmann and Rausch, {T K} and K Cloppenborg-Schmidt and J Zimmermann and D Viemann and E Herting and W G{\"o}pel and Baines, {J F} and C Kaleta and J Rupp and C H{\"a}rtel and J Pagel",
note = "{\textcopyright} The Author(s) 2018. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.",
year = "2019",
month = jul,
day = "2",
doi = "10.1093/cid/ciy882",
language = "English",
volume = "69",
pages = "268--277",
journal = "CLIN INFECT DIS",
issn = "1058-4838",
publisher = "Oxford University Press",
number = "2",

}

RIS

TY - JOUR

T1 - Gut Dysbiosis With Bacilli Dominance and Accumulation of Fermentation Products Precedes Late-onset Sepsis in Preterm Infants

AU - Graspeuntner, S

AU - Waschina, S

AU - Künzel, S

AU - Twisselmann, N

AU - Rausch, T K

AU - Cloppenborg-Schmidt, K

AU - Zimmermann, J

AU - Viemann, D

AU - Herting, E

AU - Göpel, W

AU - Baines, J F

AU - Kaleta, C

AU - Rupp, J

AU - Härtel, C

AU - Pagel, J

N1 - © The Author(s) 2018. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.

PY - 2019/7/2

Y1 - 2019/7/2

N2 - BACKGROUND: Gut dysbiosis has been suggested as a major risk factor for the development of late-onset sepsis (LOS), a main cause of mortality and morbidity in preterm infants. We aimed to assess specific signatures of the gut microbiome, including metabolic profiles, in preterm infants <34 weeks of gestation preceding LOS.METHODS: In a single-center cohort, fecal samples from preterm infants were prospectively collected during the period of highest vulnerability for LOS (days 7, 14, and 21 of life). Following 16S rRNA gene profiling, we assessed microbial community function using microbial metabolic network modeling. Data were adjusted for gestational age and use of probiotics.RESULTS: We studied stool samples from 71 preterm infants with LOS and 164 unaffected controls (no LOS/necrotizing enterocolitis). In most cases, the bacteria isolated in diagnostic blood culture corresponded to the genera in the gut microbiome. LOS cases had a decelerated development of microbial diversity. Before onset of disease, LOS cases had specific gut microbiome signatures with higher abundance of Bacilli (specifically coagulase-negative Staphylococci) and a lack of anaerobic bacteria. In silico modeling of bacterial community metabolism suggested accumulation of the fermentation products ethanol and formic acid in LOS cases before the onset of disease.CONCLUSIONS: Intestinal dysbiosis preceding LOS is characterized by an accumulation of Bacilli and their fermentation products and a paucity of anaerobic bacteria. Early microbiome and metabolic patterns may become a valuable biomarker to guide individualized prevention strategies of LOS in highly vulnerable populations.

AB - BACKGROUND: Gut dysbiosis has been suggested as a major risk factor for the development of late-onset sepsis (LOS), a main cause of mortality and morbidity in preterm infants. We aimed to assess specific signatures of the gut microbiome, including metabolic profiles, in preterm infants <34 weeks of gestation preceding LOS.METHODS: In a single-center cohort, fecal samples from preterm infants were prospectively collected during the period of highest vulnerability for LOS (days 7, 14, and 21 of life). Following 16S rRNA gene profiling, we assessed microbial community function using microbial metabolic network modeling. Data were adjusted for gestational age and use of probiotics.RESULTS: We studied stool samples from 71 preterm infants with LOS and 164 unaffected controls (no LOS/necrotizing enterocolitis). In most cases, the bacteria isolated in diagnostic blood culture corresponded to the genera in the gut microbiome. LOS cases had a decelerated development of microbial diversity. Before onset of disease, LOS cases had specific gut microbiome signatures with higher abundance of Bacilli (specifically coagulase-negative Staphylococci) and a lack of anaerobic bacteria. In silico modeling of bacterial community metabolism suggested accumulation of the fermentation products ethanol and formic acid in LOS cases before the onset of disease.CONCLUSIONS: Intestinal dysbiosis preceding LOS is characterized by an accumulation of Bacilli and their fermentation products and a paucity of anaerobic bacteria. Early microbiome and metabolic patterns may become a valuable biomarker to guide individualized prevention strategies of LOS in highly vulnerable populations.

KW - Anaerobiosis

KW - Cluster Analysis

KW - DNA, Bacterial/chemistry

KW - DNA, Ribosomal/chemistry

KW - Dysbiosis/complications

KW - Feces/chemistry

KW - Gastrointestinal Microbiome

KW - Humans

KW - Infant

KW - Infant, Newborn

KW - Infant, Premature

KW - Male

KW - Metabolome

KW - Metabolomics

KW - Metagenomics

KW - Neonatal Sepsis/pathology

KW - Phylogeny

KW - Prospective Studies

KW - RNA, Ribosomal, 16S/genetics

KW - Sequence Analysis, DNA

U2 - 10.1093/cid/ciy882

DO - 10.1093/cid/ciy882

M3 - SCORING: Journal article

C2 - 30329017

VL - 69

SP - 268

EP - 277

JO - CLIN INFECT DIS

JF - CLIN INFECT DIS

SN - 1058-4838

IS - 2

ER -