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Genomic structure and transcriptional regulation of the human NAD+-dependent 15-hydroxyprostaglandin dehydrogenase gene

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Genomic structure and transcriptional regulation of the human NAD+-dependent 15-hydroxyprostaglandin dehydrogenase gene. / Nandy, A; Jenatschke, S; Hartung, B; Milde-Langosch, K; Bamberger, A-M; Gellersen, B.

In: J MOL ENDOCRINOL, Vol. 31, No. 1, 08.2003, p. 105-21.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Nandy, A, Jenatschke, S, Hartung, B, Milde-Langosch, K, Bamberger, A-M & Gellersen, B 2003, 'Genomic structure and transcriptional regulation of the human NAD+-dependent 15-hydroxyprostaglandin dehydrogenase gene', J MOL ENDOCRINOL, vol. 31, no. 1, pp. 105-21.

APA

Nandy, A., Jenatschke, S., Hartung, B., Milde-Langosch, K., Bamberger, A-M., & Gellersen, B. (2003). Genomic structure and transcriptional regulation of the human NAD+-dependent 15-hydroxyprostaglandin dehydrogenase gene. J MOL ENDOCRINOL, 31(1), 105-21.

Vancouver

Nandy A, Jenatschke S, Hartung B, Milde-Langosch K, Bamberger A-M, Gellersen B. Genomic structure and transcriptional regulation of the human NAD+-dependent 15-hydroxyprostaglandin dehydrogenase gene. J MOL ENDOCRINOL. 2003 Aug;31(1):105-21.

Bibtex

@article{405f9a906ad946339ddf4d183f6eeb93,
title = "Genomic structure and transcriptional regulation of the human NAD+-dependent 15-hydroxyprostaglandin dehydrogenase gene",
abstract = "The NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase (PGDH) is a catabolic enzyme that controls the biological activities of prostaglandins by converting them into inactive keto-metabolites. Here we report the genomic organisation of the complete human PGDH gene and characterise its transcriptional regulation. The PGDH gene spans about 31 kb on chromosome 4 and contains 7 exons. Within 2.4 kb of the 5'-flanking sequence we identified two regions with clustered putative transcription factor binding sites. The distal promoter element PGDH-DE (positions-2152/-1944 relative to the start codon) contains binding sites for Ets and activating protein-1 (AP-1) flanked by two cAMP-responsive element-binding protein binding sites (CREB1, CREB2), whereas the proximal element PGDH-PE (-235/-153) includes an Ets and an AP-1 binding sequence. By electrophoretic mobility shift assay, no high affinity binding of Ets or AP-1 factors was observed with PGDH-PE, whereas we confirmed interaction of members of the Ets, AP-1 and CREB families of transcription factors with PGDH-DE. Transcriptional control of the PGDH promoter was assessed by transiently transfecting JEG-3 choriocarcinoma cells. A luciferase reporter gene construct containing the PGDH-PE was not induced by c-jun/c-fos in the absence or presence of co-expressed Ets-1. A construct carrying the PGDH-DE in front of the minimal homologous promoter was activated by co-transfection of expression vectors for AP-1 proteins. Mutation of the AP-1 or CREB2 site reduced the response to c-jun/c-fos, whereas mutation of the Ets site of the distal element reduced basal promoter activity. CREB activated the PGDH-DE construct through the CREB1 site. These results defined the distal element as an integrator of transcriptional regulation by AP-1, Ets and CREB proteins.",
keywords = "Base Sequence, Blotting, Southern, Cell Line, Cyclic AMP Response Element-Binding Protein, DNA, DNA Primers, Exons, Gene Amplification, Gene Expression Regulation, Enzymologic, Genes, Reporter, Humans, Hydroxyprostaglandin Dehydrogenases, Introns, Luciferases, NAD, Promoter Regions, Genetic, Recombinant Proteins, Restriction Mapping, Transcription Factor AP-1, Transcription Factors, Transcription, Genetic, Transfection",
author = "A Nandy and S Jenatschke and B Hartung and K Milde-Langosch and A-M Bamberger and B Gellersen",
year = "2003",
month = aug,
language = "English",
volume = "31",
pages = "105--21",
journal = "J MOL ENDOCRINOL",
issn = "0952-5041",
publisher = "Society for Endocrinology",
number = "1",

}

RIS

TY - JOUR

T1 - Genomic structure and transcriptional regulation of the human NAD+-dependent 15-hydroxyprostaglandin dehydrogenase gene

AU - Nandy, A

AU - Jenatschke, S

AU - Hartung, B

AU - Milde-Langosch, K

AU - Bamberger, A-M

AU - Gellersen, B

PY - 2003/8

Y1 - 2003/8

N2 - The NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase (PGDH) is a catabolic enzyme that controls the biological activities of prostaglandins by converting them into inactive keto-metabolites. Here we report the genomic organisation of the complete human PGDH gene and characterise its transcriptional regulation. The PGDH gene spans about 31 kb on chromosome 4 and contains 7 exons. Within 2.4 kb of the 5'-flanking sequence we identified two regions with clustered putative transcription factor binding sites. The distal promoter element PGDH-DE (positions-2152/-1944 relative to the start codon) contains binding sites for Ets and activating protein-1 (AP-1) flanked by two cAMP-responsive element-binding protein binding sites (CREB1, CREB2), whereas the proximal element PGDH-PE (-235/-153) includes an Ets and an AP-1 binding sequence. By electrophoretic mobility shift assay, no high affinity binding of Ets or AP-1 factors was observed with PGDH-PE, whereas we confirmed interaction of members of the Ets, AP-1 and CREB families of transcription factors with PGDH-DE. Transcriptional control of the PGDH promoter was assessed by transiently transfecting JEG-3 choriocarcinoma cells. A luciferase reporter gene construct containing the PGDH-PE was not induced by c-jun/c-fos in the absence or presence of co-expressed Ets-1. A construct carrying the PGDH-DE in front of the minimal homologous promoter was activated by co-transfection of expression vectors for AP-1 proteins. Mutation of the AP-1 or CREB2 site reduced the response to c-jun/c-fos, whereas mutation of the Ets site of the distal element reduced basal promoter activity. CREB activated the PGDH-DE construct through the CREB1 site. These results defined the distal element as an integrator of transcriptional regulation by AP-1, Ets and CREB proteins.

AB - The NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase (PGDH) is a catabolic enzyme that controls the biological activities of prostaglandins by converting them into inactive keto-metabolites. Here we report the genomic organisation of the complete human PGDH gene and characterise its transcriptional regulation. The PGDH gene spans about 31 kb on chromosome 4 and contains 7 exons. Within 2.4 kb of the 5'-flanking sequence we identified two regions with clustered putative transcription factor binding sites. The distal promoter element PGDH-DE (positions-2152/-1944 relative to the start codon) contains binding sites for Ets and activating protein-1 (AP-1) flanked by two cAMP-responsive element-binding protein binding sites (CREB1, CREB2), whereas the proximal element PGDH-PE (-235/-153) includes an Ets and an AP-1 binding sequence. By electrophoretic mobility shift assay, no high affinity binding of Ets or AP-1 factors was observed with PGDH-PE, whereas we confirmed interaction of members of the Ets, AP-1 and CREB families of transcription factors with PGDH-DE. Transcriptional control of the PGDH promoter was assessed by transiently transfecting JEG-3 choriocarcinoma cells. A luciferase reporter gene construct containing the PGDH-PE was not induced by c-jun/c-fos in the absence or presence of co-expressed Ets-1. A construct carrying the PGDH-DE in front of the minimal homologous promoter was activated by co-transfection of expression vectors for AP-1 proteins. Mutation of the AP-1 or CREB2 site reduced the response to c-jun/c-fos, whereas mutation of the Ets site of the distal element reduced basal promoter activity. CREB activated the PGDH-DE construct through the CREB1 site. These results defined the distal element as an integrator of transcriptional regulation by AP-1, Ets and CREB proteins.

KW - Base Sequence

KW - Blotting, Southern

KW - Cell Line

KW - Cyclic AMP Response Element-Binding Protein

KW - DNA

KW - DNA Primers

KW - Exons

KW - Gene Amplification

KW - Gene Expression Regulation, Enzymologic

KW - Genes, Reporter

KW - Humans

KW - Hydroxyprostaglandin Dehydrogenases

KW - Introns

KW - Luciferases

KW - NAD

KW - Promoter Regions, Genetic

KW - Recombinant Proteins

KW - Restriction Mapping

KW - Transcription Factor AP-1

KW - Transcription Factors

KW - Transcription, Genetic

KW - Transfection

M3 - SCORING: Journal article

C2 - 12914529

VL - 31

SP - 105

EP - 121

JO - J MOL ENDOCRINOL

JF - J MOL ENDOCRINOL

SN - 0952-5041

IS - 1

ER -