Genome-wide profiling of the activity-dependent hippocampal transcriptome
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Genome-wide profiling of the activity-dependent hippocampal transcriptome. / Hermey, Guido; Mahlke, Claudia; Gutzmann, Jakob J; Schreiber, Jörg; Blüthgen, Nils; Kuhl, Franz-Dietmar.
In: PLOS ONE, Vol. 8, No. 10, 01.01.2013, p. e76903.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Genome-wide profiling of the activity-dependent hippocampal transcriptome
AU - Hermey, Guido
AU - Mahlke, Claudia
AU - Gutzmann, Jakob J
AU - Schreiber, Jörg
AU - Blüthgen, Nils
AU - Kuhl, Franz-Dietmar
PY - 2013/1/1
Y1 - 2013/1/1
N2 - Activity-dependent gene expression is central for sculpting neuronal connectivity in the brain. Despite the importance for synaptic plasticity, a comprehensive analysis of the temporal changes in the transcriptomic response to neuronal activity is lacking. In a genome wide survey we identified genes that were induced at 1, 4, 8, or 24 hours following neuronal activity in the hippocampus. According to their distinct expression kinetics we assigned these genes to five clusters, each containing approximately 200 genes. Using in situ hybridizations the regulated expression of 24 genes was validated. Apart from known activity-dependent genes our study reveals a large number of unknown induced genes with distinct expression kinetics. Among these we identified several genes with complex temporal expression patterns. Furthermore, our study provides examples for activity-induced exon switching in the coding region of genes and activity-induced alternative splicing of the 3'-UTR. One example is Zwint. In contrast to the constitutively expressed variant, the induced Zwint transcript harbors multiple regulatory elements in the 3'-UTR. Taken together, our study provides a comprehensive analysis of the transcriptomic response to neuronal activity and sheds new light on expression kinetics and alternative splicing events.
AB - Activity-dependent gene expression is central for sculpting neuronal connectivity in the brain. Despite the importance for synaptic plasticity, a comprehensive analysis of the temporal changes in the transcriptomic response to neuronal activity is lacking. In a genome wide survey we identified genes that were induced at 1, 4, 8, or 24 hours following neuronal activity in the hippocampus. According to their distinct expression kinetics we assigned these genes to five clusters, each containing approximately 200 genes. Using in situ hybridizations the regulated expression of 24 genes was validated. Apart from known activity-dependent genes our study reveals a large number of unknown induced genes with distinct expression kinetics. Among these we identified several genes with complex temporal expression patterns. Furthermore, our study provides examples for activity-induced exon switching in the coding region of genes and activity-induced alternative splicing of the 3'-UTR. One example is Zwint. In contrast to the constitutively expressed variant, the induced Zwint transcript harbors multiple regulatory elements in the 3'-UTR. Taken together, our study provides a comprehensive analysis of the transcriptomic response to neuronal activity and sheds new light on expression kinetics and alternative splicing events.
KW - 3' Untranslated Regions
KW - Alternative Splicing
KW - Animals
KW - Binding Sites
KW - Cluster Analysis
KW - Exons
KW - Gene Expression Profiling
KW - Gene Expression Regulation
KW - Genome-Wide Association Study
KW - Hippocampus
KW - Male
KW - Mice
KW - Molecular Sequence Annotation
KW - Protein Binding
KW - Reproducibility of Results
KW - Transcription Factors
KW - Transcription, Genetic
KW - Transcriptome
U2 - 10.1371/journal.pone.0076903
DO - 10.1371/journal.pone.0076903
M3 - SCORING: Journal article
C2 - 24146943
VL - 8
SP - e76903
JO - PLOS ONE
JF - PLOS ONE
SN - 1932-6203
IS - 10
ER -