Genetic Labeling of Car4-expressing Cells Reveals Subpopulations of Type III Taste Cells

Kristina Lossow; Irm Hermans-Borgmeyer; Maik Behrens; Wolfgang Meyerhof

doi:10.1093/chemse/bjx048

Genetic Labeling of Car4-expressing Cells Reveals Subpopulations of Type III Taste Cells

Standard

Genetic Labeling of Car4-expressing Cells Reveals Subpopulations of Type III Taste Cells. / Lossow, Kristina; Hermans-Borgmeyer, Irm; Behrens, Maik; Meyerhof, Wolfgang.

In: CHEM SENSES, Vol. 42, No. 9, 31.10.2017, p. 747-758.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Lossow, K, Hermans-Borgmeyer, I, Behrens, M & Meyerhof, W 2017, 'Genetic Labeling of Car4-expressing Cells Reveals Subpopulations of Type III Taste Cells', CHEM SENSES, vol. 42, no. 9, pp. 747-758. https://doi.org/10.1093/chemse/bjx048

APA

Lossow, K., Hermans-Borgmeyer, I., Behrens, M., & Meyerhof, W. (2017). Genetic Labeling of Car4-expressing Cells Reveals Subpopulations of Type III Taste Cells. CHEM SENSES, 42(9), 747-758. https://doi.org/10.1093/chemse/bjx048

Vancouver

Lossow K, Hermans-Borgmeyer I, Behrens M, Meyerhof W. Genetic Labeling of Car4-expressing Cells Reveals Subpopulations of Type III Taste Cells. CHEM SENSES. 2017 Oct 31;42(9):747-758. https://doi.org/10.1093/chemse/bjx048

Bibtex

@article{35d939aaea7d4c0dba23dda0ab2a7658,

title = "Genetic Labeling of Car4-expressing Cells Reveals Subpopulations of Type III Taste Cells",

abstract = "Carbonic anhydrases form an enzyme family of 16 members, which reversibly catalyze the hydration of carbon dioxide to bicarbonate and protons. In lung, kidney, and brain, presence of carbonic anhydrases is associated with protons and bicarbonate transport in capillary endothelium of lung, reabsorption of bicarbonate in proximal renal tubules, and extracellular buffering. In contrast, their role in taste is less clear. Recently, carbonic anhydrase IV expression was detected in sour-sensing presynaptic taste cells and was associated with the taste of carbonation, yet the precise role and cell population remained uncertain. To examine the role of carbonic anhydrase 4-expressing cells in taste reception, we generated a mouse strain carrying a modified allele of the carbonic anhydrase 4 gene in which the coding region of the red fluorescent protein monomeric Cherry is attached to that of carbonic anhydrase 4 via an internal ribosome entry site. Monomeric Cherry fluorescence was detected in lingual papillae as well as taste buds of soft palate and naso-incisor duct. However, expression patterns on the tongue differ between posterior and fungiform papillae. Whereas monomeric Cherry auto-fluorescence was almost always co-localized with presynaptic cell markers aromatic L-amino-acid decarboxylase, synaptosomal-associated protein 25 or glutamic acid decarboxylase 67 in fungiform papillae and taste buds of palate and naso-incisor duct, monomeric Cherry-positive cells in posterior tongue papillae represent only a subpopulation of presynaptic cells. We conclude that this model is well suited for detailed investigation into the role of carbonic anhydrase in gustation and other processes.",

keywords = "Animals, Calcium Channels, Carbonic Anhydrases, Gene Knock-In Techniques, Genetic Engineering, In Situ Hybridization, Luminescent Proteins, Mice, Mice, 129 Strain, Mice, Inbred C57BL, Microscopy, Fluorescence, Receptors, Cell Surface, Taste Buds, Tongue, Journal Article",

author = "Kristina Lossow and Irm Hermans-Borgmeyer and Maik Behrens and Wolfgang Meyerhof",

year = "2017",

month = oct,

day = "31",

doi = "10.1093/chemse/bjx048",

language = "English",

volume = "42",

pages = "747--758",

journal = "CHEM SENSES",

issn = "0379-864X",

publisher = "Oxford University Press",

number = "9",

}

RIS

TY - JOUR

T1 - Genetic Labeling of Car4-expressing Cells Reveals Subpopulations of Type III Taste Cells

AU - Lossow, Kristina

AU - Hermans-Borgmeyer, Irm

AU - Behrens, Maik

AU - Meyerhof, Wolfgang

PY - 2017/10/31

Y1 - 2017/10/31

N2 - Carbonic anhydrases form an enzyme family of 16 members, which reversibly catalyze the hydration of carbon dioxide to bicarbonate and protons. In lung, kidney, and brain, presence of carbonic anhydrases is associated with protons and bicarbonate transport in capillary endothelium of lung, reabsorption of bicarbonate in proximal renal tubules, and extracellular buffering. In contrast, their role in taste is less clear. Recently, carbonic anhydrase IV expression was detected in sour-sensing presynaptic taste cells and was associated with the taste of carbonation, yet the precise role and cell population remained uncertain. To examine the role of carbonic anhydrase 4-expressing cells in taste reception, we generated a mouse strain carrying a modified allele of the carbonic anhydrase 4 gene in which the coding region of the red fluorescent protein monomeric Cherry is attached to that of carbonic anhydrase 4 via an internal ribosome entry site. Monomeric Cherry fluorescence was detected in lingual papillae as well as taste buds of soft palate and naso-incisor duct. However, expression patterns on the tongue differ between posterior and fungiform papillae. Whereas monomeric Cherry auto-fluorescence was almost always co-localized with presynaptic cell markers aromatic L-amino-acid decarboxylase, synaptosomal-associated protein 25 or glutamic acid decarboxylase 67 in fungiform papillae and taste buds of palate and naso-incisor duct, monomeric Cherry-positive cells in posterior tongue papillae represent only a subpopulation of presynaptic cells. We conclude that this model is well suited for detailed investigation into the role of carbonic anhydrase in gustation and other processes.

AB - Carbonic anhydrases form an enzyme family of 16 members, which reversibly catalyze the hydration of carbon dioxide to bicarbonate and protons. In lung, kidney, and brain, presence of carbonic anhydrases is associated with protons and bicarbonate transport in capillary endothelium of lung, reabsorption of bicarbonate in proximal renal tubules, and extracellular buffering. In contrast, their role in taste is less clear. Recently, carbonic anhydrase IV expression was detected in sour-sensing presynaptic taste cells and was associated with the taste of carbonation, yet the precise role and cell population remained uncertain. To examine the role of carbonic anhydrase 4-expressing cells in taste reception, we generated a mouse strain carrying a modified allele of the carbonic anhydrase 4 gene in which the coding region of the red fluorescent protein monomeric Cherry is attached to that of carbonic anhydrase 4 via an internal ribosome entry site. Monomeric Cherry fluorescence was detected in lingual papillae as well as taste buds of soft palate and naso-incisor duct. However, expression patterns on the tongue differ between posterior and fungiform papillae. Whereas monomeric Cherry auto-fluorescence was almost always co-localized with presynaptic cell markers aromatic L-amino-acid decarboxylase, synaptosomal-associated protein 25 or glutamic acid decarboxylase 67 in fungiform papillae and taste buds of palate and naso-incisor duct, monomeric Cherry-positive cells in posterior tongue papillae represent only a subpopulation of presynaptic cells. We conclude that this model is well suited for detailed investigation into the role of carbonic anhydrase in gustation and other processes.

KW - Animals

KW - Calcium Channels

KW - Carbonic Anhydrases

KW - Gene Knock-In Techniques

KW - Genetic Engineering

KW - In Situ Hybridization

KW - Luminescent Proteins

KW - Mice

KW - Mice, 129 Strain

KW - Mice, Inbred C57BL

KW - Microscopy, Fluorescence

KW - Receptors, Cell Surface

KW - Taste Buds

KW - Tongue

KW - Journal Article

U2 - 10.1093/chemse/bjx048

DO - 10.1093/chemse/bjx048

M3 - SCORING: Journal article

C2 - 29099943

VL - 42

SP - 747

EP - 758

JO - CHEM SENSES

JF - CHEM SENSES

SN - 0379-864X

IS - 9

ER -