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Generation of transgenic mice expressing FRET biosensors

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Generation of transgenic mice expressing FRET biosensors. / Hübscher, Daniela; Nikolaev, Viacheslav O.

In: Methods Mol Biol, Vol. 1294, 2015, p. 117-29.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Hübscher, D & Nikolaev, VO 2015, 'Generation of transgenic mice expressing FRET biosensors', Methods Mol Biol, vol. 1294, pp. 117-29. https://doi.org/10.1007/978-1-4939-2537-7_9

APA

Hübscher, D., & Nikolaev, V. O. (2015). Generation of transgenic mice expressing FRET biosensors. Methods Mol Biol, 1294, 117-29. https://doi.org/10.1007/978-1-4939-2537-7_9

Vancouver

Hübscher D, Nikolaev VO. Generation of transgenic mice expressing FRET biosensors. Methods Mol Biol. 2015;1294:117-29. https://doi.org/10.1007/978-1-4939-2537-7_9

Bibtex

@article{ab20839aae16412fa82dfa60681219d5,
title = "Generation of transgenic mice expressing FRET biosensors",
abstract = "Transgenic mice play a significant role in modern biomedical research. They allow not only mechanistic insights into the functions of specific genes and proteins. Recent strategies have also established the use of transgenic mice as an exciting tool for the expression and in vivo or in situ analysis of fluorescent biosensors, which are capable of directly reporting second messenger levels and biochemical processes in real time and in living cells. In this chapter, we present a detailed protocol for the generation of plasmid vectors and transgenic mice expressing a F{\"o}rster resonance energy transfer (FRET)-based biosensor for the second messenger 3',5'-cyclic adenosine monophosphate. These tools and techniques should provide great potential for the analysis of second messenger dynamics in a more physiologically relevant context.",
keywords = "Animals, Biosensing Techniques, Cloning, Molecular, Cyclic AMP, Fluorescence Resonance Energy Transfer, Genetic Vectors, Mice, Mice, Transgenic, Plasmids",
author = "Daniela H{\"u}bscher and Nikolaev, {Viacheslav O}",
year = "2015",
doi = "10.1007/978-1-4939-2537-7_9",
language = "English",
volume = "1294",
pages = "117--29",
journal = "Methods Mol Biol",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Generation of transgenic mice expressing FRET biosensors

AU - Hübscher, Daniela

AU - Nikolaev, Viacheslav O

PY - 2015

Y1 - 2015

N2 - Transgenic mice play a significant role in modern biomedical research. They allow not only mechanistic insights into the functions of specific genes and proteins. Recent strategies have also established the use of transgenic mice as an exciting tool for the expression and in vivo or in situ analysis of fluorescent biosensors, which are capable of directly reporting second messenger levels and biochemical processes in real time and in living cells. In this chapter, we present a detailed protocol for the generation of plasmid vectors and transgenic mice expressing a Förster resonance energy transfer (FRET)-based biosensor for the second messenger 3',5'-cyclic adenosine monophosphate. These tools and techniques should provide great potential for the analysis of second messenger dynamics in a more physiologically relevant context.

AB - Transgenic mice play a significant role in modern biomedical research. They allow not only mechanistic insights into the functions of specific genes and proteins. Recent strategies have also established the use of transgenic mice as an exciting tool for the expression and in vivo or in situ analysis of fluorescent biosensors, which are capable of directly reporting second messenger levels and biochemical processes in real time and in living cells. In this chapter, we present a detailed protocol for the generation of plasmid vectors and transgenic mice expressing a Förster resonance energy transfer (FRET)-based biosensor for the second messenger 3',5'-cyclic adenosine monophosphate. These tools and techniques should provide great potential for the analysis of second messenger dynamics in a more physiologically relevant context.

KW - Animals

KW - Biosensing Techniques

KW - Cloning, Molecular

KW - Cyclic AMP

KW - Fluorescence Resonance Energy Transfer

KW - Genetic Vectors

KW - Mice

KW - Mice, Transgenic

KW - Plasmids

U2 - 10.1007/978-1-4939-2537-7_9

DO - 10.1007/978-1-4939-2537-7_9

M3 - SCORING: Journal article

C2 - 25783881

VL - 1294

SP - 117

EP - 129

JO - Methods Mol Biol

JF - Methods Mol Biol

SN - 1064-3745

ER -