Generation and characterization of three new monoclonal antibodies detecting the allospecificities HLA-A2,w69, HLA-A3 and HLA-B13.

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Generation and characterization of three new monoclonal antibodies detecting the allospecificities HLA-A2,w69, HLA-A3 and HLA-B13. / Eiermann, Thomas; Raghavachar, A; Wölpl, A; Ballas, M; Fischer, M; Goldmann, S F.

In: TISSUE ANTIGENS, Vol. 29, No. 4, 4, 1987, p. 201-207.

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@article{bd5408abe5a541aba04ffeb4455ee0f2,
title = "Generation and characterization of three new monoclonal antibodies detecting the allospecificities HLA-A2,w69, HLA-A3 and HLA-B13.",
abstract = "Immunization of balb/c mice with peripheral blood mononuclear cells of a leukemic patient possessing the antigens HLA-A2,3; B7,35 resulted in the polymorphic monoclonal antibody (moab) UL-101/68 (IgM) defining HLA-A3. Immunization of a second group of balb/c mice with the lymphoblastoid cell line BER homozygous for HLA-A2; B13 revealed two polymorphic moabs UL-39/10 (IgG3) specific for HLA-B13 and UL-39/24 (IgG2b) defining HLA-A2,w69. Immunoprecipitation and polyacrylamide gel electrophoresis with the two moabs of the IgG isotype confirmed the class I structure of the recognized antigens.",
author = "Thomas Eiermann and A Raghavachar and A W{\"o}lpl and M Ballas and M Fischer and Goldmann, {S F}",
year = "1987",
language = "Deutsch",
volume = "29",
pages = "201--207",
journal = "TISSUE ANTIGENS",
issn = "0001-2815",
publisher = "Wiley-Blackwell",
number = "4",

}

RIS

TY - JOUR

T1 - Generation and characterization of three new monoclonal antibodies detecting the allospecificities HLA-A2,w69, HLA-A3 and HLA-B13.

AU - Eiermann, Thomas

AU - Raghavachar, A

AU - Wölpl, A

AU - Ballas, M

AU - Fischer, M

AU - Goldmann, S F

PY - 1987

Y1 - 1987

N2 - Immunization of balb/c mice with peripheral blood mononuclear cells of a leukemic patient possessing the antigens HLA-A2,3; B7,35 resulted in the polymorphic monoclonal antibody (moab) UL-101/68 (IgM) defining HLA-A3. Immunization of a second group of balb/c mice with the lymphoblastoid cell line BER homozygous for HLA-A2; B13 revealed two polymorphic moabs UL-39/10 (IgG3) specific for HLA-B13 and UL-39/24 (IgG2b) defining HLA-A2,w69. Immunoprecipitation and polyacrylamide gel electrophoresis with the two moabs of the IgG isotype confirmed the class I structure of the recognized antigens.

AB - Immunization of balb/c mice with peripheral blood mononuclear cells of a leukemic patient possessing the antigens HLA-A2,3; B7,35 resulted in the polymorphic monoclonal antibody (moab) UL-101/68 (IgM) defining HLA-A3. Immunization of a second group of balb/c mice with the lymphoblastoid cell line BER homozygous for HLA-A2; B13 revealed two polymorphic moabs UL-39/10 (IgG3) specific for HLA-B13 and UL-39/24 (IgG2b) defining HLA-A2,w69. Immunoprecipitation and polyacrylamide gel electrophoresis with the two moabs of the IgG isotype confirmed the class I structure of the recognized antigens.

M3 - SCORING: Zeitschriftenaufsatz

VL - 29

SP - 201

EP - 207

JO - TISSUE ANTIGENS

JF - TISSUE ANTIGENS

SN - 0001-2815

IS - 4

M1 - 4

ER -