Generation and Characterization of Specific Monoclonal Antibodies and Nanobodies Directed Against the ATP-Gated Channel P2X4
Standard
Generation and Characterization of Specific Monoclonal Antibodies and Nanobodies Directed Against the ATP-Gated Channel P2X4. / Bergmann, Philine; Garcia de Paco, Elvira; Rissiek, Björn; Menzel, Stephan; Dubberke, Gudrun; Hua, Jennifer; Rassendren, François; Ulmann, Lauriane; Koch-Nolte, Friedrich.
In: FRONT CELL NEUROSCI, Vol. 13, 2019, p. 498.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Generation and Characterization of Specific Monoclonal Antibodies and Nanobodies Directed Against the ATP-Gated Channel P2X4
AU - Bergmann, Philine
AU - Garcia de Paco, Elvira
AU - Rissiek, Björn
AU - Menzel, Stephan
AU - Dubberke, Gudrun
AU - Hua, Jennifer
AU - Rassendren, François
AU - Ulmann, Lauriane
AU - Koch-Nolte, Friedrich
N1 - Copyright © 2019 Bergmann, Garcia de Paco, Rissiek, Menzel, Dubberke, Hua, Rassendren, Ulmann and Koch-Nolte.
PY - 2019
Y1 - 2019
N2 - The P2X4 channel is involved in different physiological and pathological conditions and functions in the nervous system. Despite the existence of several mouse models for which the expression of the gene was manipulated, there is still little information on the expression of the protein at the cellular level. In particular, supposedly specific available antibodies have often proved to recognize unrelated proteins in P2X4-deficient mice. Here, we used an in vivo DNA vaccine approach to generate a series of monoclonal antibodies and nanobodies specific for human, mouse, and rat P2X4 channels. We further characterized these antibodies and show that they solely recognize the native form of the proteins both in biochemical and cytometric applications. Some of these antibodies prove to specifically recognize P2X4 channels by immunostaining in brain or sensory ganglia slices, as well as at the cellular and subcellular levels. Due to their clonality, these different antibodies should represent versatile tools for further characterizing the cellular functions of P2X4 in the nervous system as well as at the periphery.
AB - The P2X4 channel is involved in different physiological and pathological conditions and functions in the nervous system. Despite the existence of several mouse models for which the expression of the gene was manipulated, there is still little information on the expression of the protein at the cellular level. In particular, supposedly specific available antibodies have often proved to recognize unrelated proteins in P2X4-deficient mice. Here, we used an in vivo DNA vaccine approach to generate a series of monoclonal antibodies and nanobodies specific for human, mouse, and rat P2X4 channels. We further characterized these antibodies and show that they solely recognize the native form of the proteins both in biochemical and cytometric applications. Some of these antibodies prove to specifically recognize P2X4 channels by immunostaining in brain or sensory ganglia slices, as well as at the cellular and subcellular levels. Due to their clonality, these different antibodies should represent versatile tools for further characterizing the cellular functions of P2X4 in the nervous system as well as at the periphery.
U2 - 10.3389/fncel.2019.00498
DO - 10.3389/fncel.2019.00498
M3 - SCORING: Journal article
C2 - 31798414
VL - 13
SP - 498
JO - FRONT CELL NEUROSCI
JF - FRONT CELL NEUROSCI
SN - 1662-5102
ER -
