Gene amplification in ductal carcinoma in situ of the breast.
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Gene amplification in ductal carcinoma in situ of the breast. / Burkhardt, Lia; Grob, Tobias; Hermann, I; Burandt, Eike Christian; Choschzick, Matthias; Jänicke, Fritz; Müller, Volkmar; Bokemeyer, Carsten; Simon, Ronald; Sauter, Guido; Wilczak, W; Lebeau, Annette.
In: BREAST CANCER RES TR, 2009.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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T1 - Gene amplification in ductal carcinoma in situ of the breast.
AU - Burkhardt, Lia
AU - Grob, Tobias
AU - Hermann, I
AU - Burandt, Eike Christian
AU - Choschzick, Matthias
AU - Jänicke, Fritz
AU - Müller, Volkmar
AU - Bokemeyer, Carsten
AU - Simon, Ronald
AU - Sauter, Guido
AU - Wilczak, W
AU - Lebeau, Annette
PY - 2009
Y1 - 2009
N2 - Multiple different biologically and clinically relevant genes are often amplified in invasive breast cancer, including HER2, ESR1, CCND1, and MYC. So far, little is known about their role in tumor progression. To investigate their significance for tumor invasion, we compared pure ductal carcinoma in situ (DCIS) and DCIS associated with invasive cancer with regard to the amplification of these genes. Fluorescence in situ hybridization (FISH) was performed on a tissue microarray containing samples from 130 pure DCIS and 159 DCIS associated with invasive breast cancer. Of the latter patients, we analyzed the intraductal and invasive components separately. In addition, lymph node metastases of 23 patients with invasive carcinoma were included. Amplification rates of pure DCIS and DCIS associated with invasive cancer did not differ significantly (pure DCIS vs. DCIS associated with invasive cancer: HER2 22.7 vs. 24.2%, ESR1 19.0 vs. 24.1%, CCND1 10.0 vs. 14.8%, MYC 11.8 vs. 6.5%; P > 0.05). Furthermore, we observed a high concordance of the amplification status for all genes if in situ and invasive carcinoma of individual patients were compared. This applied also to the corresponding lymph node metastases. Our results indicate no significant differences between the gene amplification status of DCIS and invasive breast cancer concerning HER2, ESR1, CCND1, and MYC. Therefore, our data suggest an early role of all analyzed gene amplifications in breast cancer development but not in the initiation of invasive tumor growth.
AB - Multiple different biologically and clinically relevant genes are often amplified in invasive breast cancer, including HER2, ESR1, CCND1, and MYC. So far, little is known about their role in tumor progression. To investigate their significance for tumor invasion, we compared pure ductal carcinoma in situ (DCIS) and DCIS associated with invasive cancer with regard to the amplification of these genes. Fluorescence in situ hybridization (FISH) was performed on a tissue microarray containing samples from 130 pure DCIS and 159 DCIS associated with invasive breast cancer. Of the latter patients, we analyzed the intraductal and invasive components separately. In addition, lymph node metastases of 23 patients with invasive carcinoma were included. Amplification rates of pure DCIS and DCIS associated with invasive cancer did not differ significantly (pure DCIS vs. DCIS associated with invasive cancer: HER2 22.7 vs. 24.2%, ESR1 19.0 vs. 24.1%, CCND1 10.0 vs. 14.8%, MYC 11.8 vs. 6.5%; P > 0.05). Furthermore, we observed a high concordance of the amplification status for all genes if in situ and invasive carcinoma of individual patients were compared. This applied also to the corresponding lymph node metastases. Our results indicate no significant differences between the gene amplification status of DCIS and invasive breast cancer concerning HER2, ESR1, CCND1, and MYC. Therefore, our data suggest an early role of all analyzed gene amplifications in breast cancer development but not in the initiation of invasive tumor growth.
M3 - SCORING: Zeitschriftenaufsatz
JO - BREAST CANCER RES TR
JF - BREAST CANCER RES TR
SN - 0167-6806
ER -
