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Gamma delta lymphocytes in endocrine autoimmunity

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Gamma delta lymphocytes in endocrine autoimmunity : evidence of expansion in Graves' disease but not in type 1 diabetes. / Roura-Mir, I C; Alcalde, L; Vargas, F; Tolosa, E; Obiols, G; Foz, M; Jaraquemada, D; Pujol-Borrell, R.

In: CLIN EXP IMMUNOL, Vol. 92, No. 2, 01.05.1993, p. 288-95.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Roura-Mir, IC, Alcalde, L, Vargas, F, Tolosa, E, Obiols, G, Foz, M, Jaraquemada, D & Pujol-Borrell, R 1993, 'Gamma delta lymphocytes in endocrine autoimmunity: evidence of expansion in Graves' disease but not in type 1 diabetes', CLIN EXP IMMUNOL, vol. 92, no. 2, pp. 288-95.

APA

Roura-Mir, I. C., Alcalde, L., Vargas, F., Tolosa, E., Obiols, G., Foz, M., Jaraquemada, D., & Pujol-Borrell, R. (1993). Gamma delta lymphocytes in endocrine autoimmunity: evidence of expansion in Graves' disease but not in type 1 diabetes. CLIN EXP IMMUNOL, 92(2), 288-95.

Vancouver

Roura-Mir IC, Alcalde L, Vargas F, Tolosa E, Obiols G, Foz M et al. Gamma delta lymphocytes in endocrine autoimmunity: evidence of expansion in Graves' disease but not in type 1 diabetes. CLIN EXP IMMUNOL. 1993 May 1;92(2):288-95.

Bibtex

@article{dea6e4016f204222a3f36c8937ec043a,
title = "Gamma delta lymphocytes in endocrine autoimmunity: evidence of expansion in Graves' disease but not in type 1 diabetes",
abstract = "Endocrine autoimmune disorders are mediated by T cell-dependent responses to organ-specific antigens, but the mechanisms initiating the process remain unknown. Lymphocytes which use the gamma delta heterodimer as T cell receptor (TCR) for antigen constitute a distinct subset of T cells whose function remains elusive. In order to investigate their possible involvement in endocrine autoimmunity we have determined the proportion of gamma delta T cells in the peripheral blood of 23 patients with type 1 (insulin-dependent) diabetes mellitus (type-1 DM) and 30 patients with autoimmune thyrotoxicosis (Graves' disease). T lymphocyte TCR expression was assessed by fluorescence-activated flow cytometry on peripheral blood mononuclear cells using MoAbs UCHT1 (CD3), TCR delta 1 (gamma delta TCR), WT31 and beta F1 (alpha beta TCR) and both the percentage of T cells expressing gamma delta and the ratio gamma delta/alpha beta were calculated. In the diabetic patients gamma delta cells were not significantly different from the control group (7.7 +/- 54% versus 8.0 +/- 5.5% of T cells, P NS). There was no relation between the proportion of gamma delta lymphocytes and the presence of islet cell antibodies (ICA) in the sera. The Graves' patients showed a tendency towards a higher proportion of gamma delta T lymphocytes than the controls (gamma delta/alpha beta ratios: 0.095 +/- 0.047 versus 0.063 +/- 0.022, P = 0.03). In 14 Graves' patients the number of gamma delta were measured in paired samples of peripheral and intrathyroidal lymphocytes, demonstrating an expansion of gamma delta within the thyroid glands (0.21 +/- 0.3 versus 0.095 +/- 0.047, P = 0.032). Immunohistochemical studies showed that gamma delta cells were scattered among the predominant alpha beta lymphocytes infiltrating the thyroid gland and that they account for 10% of intraepithelial lymphocytes. No relation was found between the increase of gamma delta lymphocytes and any clinical features.",
keywords = "Adolescent, Adult, Age Factors, Autoimmune Diseases, Child, Diabetes Mellitus, Type 1, Female, Fluorescent Antibody Technique, Graves Disease, Humans, Immunophenotyping, Male, Middle Aged, Receptors, Antigen, T-Cell, gamma-delta, T-Lymphocyte Subsets, Thyroid Gland",
author = "Roura-Mir, {I C} and L Alcalde and F Vargas and E Tolosa and G Obiols and M Foz and D Jaraquemada and R Pujol-Borrell",
year = "1993",
month = may,
day = "1",
language = "English",
volume = "92",
pages = "288--95",
journal = "CLIN EXP IMMUNOL",
issn = "0009-9104",
publisher = "Wiley-Blackwell",
number = "2",

}

RIS

TY - JOUR

T1 - Gamma delta lymphocytes in endocrine autoimmunity

T2 - evidence of expansion in Graves' disease but not in type 1 diabetes

AU - Roura-Mir, I C

AU - Alcalde, L

AU - Vargas, F

AU - Tolosa, E

AU - Obiols, G

AU - Foz, M

AU - Jaraquemada, D

AU - Pujol-Borrell, R

PY - 1993/5/1

Y1 - 1993/5/1

N2 - Endocrine autoimmune disorders are mediated by T cell-dependent responses to organ-specific antigens, but the mechanisms initiating the process remain unknown. Lymphocytes which use the gamma delta heterodimer as T cell receptor (TCR) for antigen constitute a distinct subset of T cells whose function remains elusive. In order to investigate their possible involvement in endocrine autoimmunity we have determined the proportion of gamma delta T cells in the peripheral blood of 23 patients with type 1 (insulin-dependent) diabetes mellitus (type-1 DM) and 30 patients with autoimmune thyrotoxicosis (Graves' disease). T lymphocyte TCR expression was assessed by fluorescence-activated flow cytometry on peripheral blood mononuclear cells using MoAbs UCHT1 (CD3), TCR delta 1 (gamma delta TCR), WT31 and beta F1 (alpha beta TCR) and both the percentage of T cells expressing gamma delta and the ratio gamma delta/alpha beta were calculated. In the diabetic patients gamma delta cells were not significantly different from the control group (7.7 +/- 54% versus 8.0 +/- 5.5% of T cells, P NS). There was no relation between the proportion of gamma delta lymphocytes and the presence of islet cell antibodies (ICA) in the sera. The Graves' patients showed a tendency towards a higher proportion of gamma delta T lymphocytes than the controls (gamma delta/alpha beta ratios: 0.095 +/- 0.047 versus 0.063 +/- 0.022, P = 0.03). In 14 Graves' patients the number of gamma delta were measured in paired samples of peripheral and intrathyroidal lymphocytes, demonstrating an expansion of gamma delta within the thyroid glands (0.21 +/- 0.3 versus 0.095 +/- 0.047, P = 0.032). Immunohistochemical studies showed that gamma delta cells were scattered among the predominant alpha beta lymphocytes infiltrating the thyroid gland and that they account for 10% of intraepithelial lymphocytes. No relation was found between the increase of gamma delta lymphocytes and any clinical features.

AB - Endocrine autoimmune disorders are mediated by T cell-dependent responses to organ-specific antigens, but the mechanisms initiating the process remain unknown. Lymphocytes which use the gamma delta heterodimer as T cell receptor (TCR) for antigen constitute a distinct subset of T cells whose function remains elusive. In order to investigate their possible involvement in endocrine autoimmunity we have determined the proportion of gamma delta T cells in the peripheral blood of 23 patients with type 1 (insulin-dependent) diabetes mellitus (type-1 DM) and 30 patients with autoimmune thyrotoxicosis (Graves' disease). T lymphocyte TCR expression was assessed by fluorescence-activated flow cytometry on peripheral blood mononuclear cells using MoAbs UCHT1 (CD3), TCR delta 1 (gamma delta TCR), WT31 and beta F1 (alpha beta TCR) and both the percentage of T cells expressing gamma delta and the ratio gamma delta/alpha beta were calculated. In the diabetic patients gamma delta cells were not significantly different from the control group (7.7 +/- 54% versus 8.0 +/- 5.5% of T cells, P NS). There was no relation between the proportion of gamma delta lymphocytes and the presence of islet cell antibodies (ICA) in the sera. The Graves' patients showed a tendency towards a higher proportion of gamma delta T lymphocytes than the controls (gamma delta/alpha beta ratios: 0.095 +/- 0.047 versus 0.063 +/- 0.022, P = 0.03). In 14 Graves' patients the number of gamma delta were measured in paired samples of peripheral and intrathyroidal lymphocytes, demonstrating an expansion of gamma delta within the thyroid glands (0.21 +/- 0.3 versus 0.095 +/- 0.047, P = 0.032). Immunohistochemical studies showed that gamma delta cells were scattered among the predominant alpha beta lymphocytes infiltrating the thyroid gland and that they account for 10% of intraepithelial lymphocytes. No relation was found between the increase of gamma delta lymphocytes and any clinical features.

KW - Adolescent

KW - Adult

KW - Age Factors

KW - Autoimmune Diseases

KW - Child

KW - Diabetes Mellitus, Type 1

KW - Female

KW - Fluorescent Antibody Technique

KW - Graves Disease

KW - Humans

KW - Immunophenotyping

KW - Male

KW - Middle Aged

KW - Receptors, Antigen, T-Cell, gamma-delta

KW - T-Lymphocyte Subsets

KW - Thyroid Gland

M3 - SCORING: Journal article

C2 - 8485915

VL - 92

SP - 288

EP - 295

JO - CLIN EXP IMMUNOL

JF - CLIN EXP IMMUNOL

SN - 0009-9104

IS - 2

ER -