Research ExplorerPublicationsFunctional studies of synthetic gramicidin hybrid ion channe...

Functional studies of synthetic gramicidin hybrid ion channels in CHO cells.

Standard

Functional studies of synthetic gramicidin hybrid ion channels in CHO cells. / Wesolowski, Ryszard; Sommer, Anna; Arndt, Hans-Dieter; Koert, Ulrich; Reiss, Philipp; Wimmers, Sönke; Strauss, Olaf.

In: CHEMBIOCHEM, Vol. 8, No. 5, 5, 2007, p. 513-520.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Wesolowski, R, Sommer, A, Arndt, H-D, Koert, U, Reiss, P, Wimmers, S & Strauss, O 2007, 'Functional studies of synthetic gramicidin hybrid ion channels in CHO cells.', CHEMBIOCHEM, vol. 8, no. 5, 5, pp. 513-520. <http://www.ncbi.nlm.nih.gov/pubmed/17300127?dopt=Citation>

APA

Wesolowski, R., Sommer, A., Arndt, H-D., Koert, U., Reiss, P., Wimmers, S., & Strauss, O. (2007). Functional studies of synthetic gramicidin hybrid ion channels in CHO cells. CHEMBIOCHEM, 8(5), 513-520. [5]. http://www.ncbi.nlm.nih.gov/pubmed/17300127?dopt=Citation

Vancouver

Wesolowski R, Sommer A, Arndt H-D, Koert U, Reiss P, Wimmers S et al. Functional studies of synthetic gramicidin hybrid ion channels in CHO cells. CHEMBIOCHEM. 2007;8(5):513-520. 5.

Bibtex

@article{55fd1e39f52f4de6bd793a94210de377,
title = "Functional studies of synthetic gramicidin hybrid ion channels in CHO cells.",
abstract = "The function of a gramicidin hybrid ion channel in living Chinese hamster ovary (CHO) cells was investigated by the patch clamp method. The synthetic ion channel 1 consists of two cyclohexyl ether amino acids that link two mini-gramicidin strands. With 1 at a concentration of 1.0 microM, an increase in the whole-cell membrane conductance was observed after 1.37 min. The conductance showed larger currents when Cs(+) was used as charge carrier than when Na(+) and K(+) were used. In single-channel recordings with Cs(+) as charge carrier, the substance showed comparable single-channel amplitudes in the membrane of living cells and artificial black lipid bilayers. In addition to functioning as a cation channel, compound 1 appeared to be a water channel. Exposure of the CHO cells to an extracellular hypoosmotic solution did not substantially change the cell volume. Extracellular hypoosmotic conditions in the presence of 1 increased the cell size to 146.5 % that of the control. Thus, the synthetic hybrid channel 1 can function as a cation channel with some Cs(+) specificity, and as a water channel in CHO cells.",
author = "Ryszard Wesolowski and Anna Sommer and Hans-Dieter Arndt and Ulrich Koert and Philipp Reiss and S{\"o}nke Wimmers and Olaf Strauss",
year = "2007",
language = "Deutsch",
volume = "8",
pages = "513--520",
journal = "CHEMBIOCHEM",
issn = "1439-4227",
publisher = "Wiley-VCH Verlag GmbH",
number = "5",

}

RIS

TY - JOUR

T1 - Functional studies of synthetic gramicidin hybrid ion channels in CHO cells.

AU - Wesolowski, Ryszard

AU - Sommer, Anna

AU - Arndt, Hans-Dieter

AU - Koert, Ulrich

AU - Reiss, Philipp

AU - Wimmers, Sönke

AU - Strauss, Olaf

PY - 2007

Y1 - 2007

N2 - The function of a gramicidin hybrid ion channel in living Chinese hamster ovary (CHO) cells was investigated by the patch clamp method. The synthetic ion channel 1 consists of two cyclohexyl ether amino acids that link two mini-gramicidin strands. With 1 at a concentration of 1.0 microM, an increase in the whole-cell membrane conductance was observed after 1.37 min. The conductance showed larger currents when Cs(+) was used as charge carrier than when Na(+) and K(+) were used. In single-channel recordings with Cs(+) as charge carrier, the substance showed comparable single-channel amplitudes in the membrane of living cells and artificial black lipid bilayers. In addition to functioning as a cation channel, compound 1 appeared to be a water channel. Exposure of the CHO cells to an extracellular hypoosmotic solution did not substantially change the cell volume. Extracellular hypoosmotic conditions in the presence of 1 increased the cell size to 146.5 % that of the control. Thus, the synthetic hybrid channel 1 can function as a cation channel with some Cs(+) specificity, and as a water channel in CHO cells.

AB - The function of a gramicidin hybrid ion channel in living Chinese hamster ovary (CHO) cells was investigated by the patch clamp method. The synthetic ion channel 1 consists of two cyclohexyl ether amino acids that link two mini-gramicidin strands. With 1 at a concentration of 1.0 microM, an increase in the whole-cell membrane conductance was observed after 1.37 min. The conductance showed larger currents when Cs(+) was used as charge carrier than when Na(+) and K(+) were used. In single-channel recordings with Cs(+) as charge carrier, the substance showed comparable single-channel amplitudes in the membrane of living cells and artificial black lipid bilayers. In addition to functioning as a cation channel, compound 1 appeared to be a water channel. Exposure of the CHO cells to an extracellular hypoosmotic solution did not substantially change the cell volume. Extracellular hypoosmotic conditions in the presence of 1 increased the cell size to 146.5 % that of the control. Thus, the synthetic hybrid channel 1 can function as a cation channel with some Cs(+) specificity, and as a water channel in CHO cells.

M3 - SCORING: Zeitschriftenaufsatz

VL - 8

SP - 513

EP - 520

JO - CHEMBIOCHEM

JF - CHEMBIOCHEM

SN - 1439-4227

IS - 5

M1 - 5

ER -