Functional interaction of SCAI with the SWI/SNF complex for transcription and tumor cell invasion
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Functional interaction of SCAI with the SWI/SNF complex for transcription and tumor cell invasion. / Kreßner, Camilla; Nollau, Peter; Grosse, Robert; Brandt, Dominique T.
In: PLOS ONE, Vol. 8, No. 8, 01.01.2013, p. e69947.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Functional interaction of SCAI with the SWI/SNF complex for transcription and tumor cell invasion
AU - Kreßner, Camilla
AU - Nollau, Peter
AU - Grosse, Robert
AU - Brandt, Dominique T
PY - 2013/1/1
Y1 - 2013/1/1
N2 - We have recently characterized SCAI (Suppressor of Cancer Cell Invasion), a transcriptional modulator regulating cancer cell motility through suppression of MAL/SRF dependent gene transcription. We show here that SCAI is expressed in a wide range of normal human tissues and its expression is diminished in a large array of primary human breast cancer samples indicating that SCAI expression might be linked to the etiology of human cancer. To establish a functional link between SCAI and tumorigenesis we performed affinity columns to identify SCAI-interacting proteins. Our data show that SCAI interacts with the tumor suppressing SWI/SNF chromatin remodeling complex to promote changes in gene expression and the invasive capacities of human tumor cells. Moreover our data implicate a functional hierarchy between SCAI and BRM, since SCAI function is abrogated in the absence of BRM expression.
AB - We have recently characterized SCAI (Suppressor of Cancer Cell Invasion), a transcriptional modulator regulating cancer cell motility through suppression of MAL/SRF dependent gene transcription. We show here that SCAI is expressed in a wide range of normal human tissues and its expression is diminished in a large array of primary human breast cancer samples indicating that SCAI expression might be linked to the etiology of human cancer. To establish a functional link between SCAI and tumorigenesis we performed affinity columns to identify SCAI-interacting proteins. Our data show that SCAI interacts with the tumor suppressing SWI/SNF chromatin remodeling complex to promote changes in gene expression and the invasive capacities of human tumor cells. Moreover our data implicate a functional hierarchy between SCAI and BRM, since SCAI function is abrogated in the absence of BRM expression.
KW - Breast Neoplasms
KW - Cell Movement
KW - Cells, Cultured
KW - Chromosomal Proteins, Non-Histone
KW - Female
KW - Genes, Reporter
KW - HEK293 Cells
KW - Humans
KW - Immunoprecipitation
KW - Neoplasm Invasiveness
KW - Transcription Factors
KW - Transcription, Genetic
KW - Tumor Suppressor Proteins
U2 - 10.1371/journal.pone.0069947
DO - 10.1371/journal.pone.0069947
M3 - SCORING: Journal article
C2 - 23936361
VL - 8
SP - e69947
JO - PLOS ONE
JF - PLOS ONE
SN - 1932-6203
IS - 8
ER -