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Fully automated interpretation of ionizing radiation-induced γH2AX foci by the novel pattern recognition system AKLIDES(®).

Standard

Fully automated interpretation of ionizing radiation-induced γH2AX foci by the novel pattern recognition system AKLIDES(®). / Runge, Roswitha; Hiemann, Rico; Wendisch, Maria; Kasten-Pisula, Ulla; Storch, Katja; Zoephel, Klaus; Fritz, Christina; Roggenbuck, Dirk; Wunderlich, Gerd; Conrad, Karsten; Kotzerke, Joerg.

In: INT J RADIAT BIOL, Vol. 88, No. 5, 5, 2012, p. 439-447.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Runge, R, Hiemann, R, Wendisch, M, Kasten-Pisula, U, Storch, K, Zoephel, K, Fritz, C, Roggenbuck, D, Wunderlich, G, Conrad, K & Kotzerke, J 2012, 'Fully automated interpretation of ionizing radiation-induced γH2AX foci by the novel pattern recognition system AKLIDES(®).', INT J RADIAT BIOL, vol. 88, no. 5, 5, pp. 439-447. <http://www.ncbi.nlm.nih.gov/pubmed/22280362?dopt=Citation>

APA

Runge, R., Hiemann, R., Wendisch, M., Kasten-Pisula, U., Storch, K., Zoephel, K., Fritz, C., Roggenbuck, D., Wunderlich, G., Conrad, K., & Kotzerke, J. (2012). Fully automated interpretation of ionizing radiation-induced γH2AX foci by the novel pattern recognition system AKLIDES(®). INT J RADIAT BIOL, 88(5), 439-447. [5]. http://www.ncbi.nlm.nih.gov/pubmed/22280362?dopt=Citation

Vancouver

Runge R, Hiemann R, Wendisch M, Kasten-Pisula U, Storch K, Zoephel K et al. Fully automated interpretation of ionizing radiation-induced γH2AX foci by the novel pattern recognition system AKLIDES(®). INT J RADIAT BIOL. 2012;88(5):439-447. 5.

Bibtex

@article{4152833ef691400aaf9e1e1329087ce1,
title = "Fully automated interpretation of ionizing radiation-induced γH2AX foci by the novel pattern recognition system AKLIDES({\textregistered}).",
abstract = "Purpose: Assessment of phosphorylated histone H2AX (?H2AX) foci as a measure for double-strand breaks (DSB) is a common technique. Since visual interpretation is time-consuming and influenced by subjective factors, we adapted the pattern recognition algorithms of autoantibodies to automated reading of ?H2AX foci. Materials and methods: DSB formation was assessed by detection of ?H2AX foci after exposition of thyreocyte rat cell line to (188)Re. We used pattern recognition algorithms of the automated fluorescence interpretation system AKLIDES({\textregistered}) for evaluation of ?H2AX foci. Manual investigation was performed by three laboratories involving five observers. The results were compared by determining correlation and inter-laboratory variability. Results: The study confirmed the adaptation of automated interpretation system AKLIDES{\textregistered} to automated assessment of ?H2AX foci in irradiated cells. Both manual and automated quantification resulted in increasing focus numbers depending on dose. Comparison of automated reading with visual assessment for five manual observers resulted in a determination coefficient of R(2) =?0.889. The inter-laboratory variability for five manual investigators of three laboratories was 38.4 %. Conclusion: The interpretation system AKLIDES({\textregistered}) demonstrated a high correlation with visually observed results. High inter-laboratory variability found for manual investigations revealed the usefulness for a standardized technique for evaluation of ?H2AX foci.",
author = "Roswitha Runge and Rico Hiemann and Maria Wendisch and Ulla Kasten-Pisula and Katja Storch and Klaus Zoephel and Christina Fritz and Dirk Roggenbuck and Gerd Wunderlich and Karsten Conrad and Joerg Kotzerke",
year = "2012",
language = "English",
volume = "88",
pages = "439--447",
journal = "INT J RADIAT BIOL",
issn = "0955-3002",
publisher = "Taylor & Francis",
number = "5",

}

RIS

TY - JOUR

T1 - Fully automated interpretation of ionizing radiation-induced γH2AX foci by the novel pattern recognition system AKLIDES(®).

AU - Runge, Roswitha

AU - Hiemann, Rico

AU - Wendisch, Maria

AU - Kasten-Pisula, Ulla

AU - Storch, Katja

AU - Zoephel, Klaus

AU - Fritz, Christina

AU - Roggenbuck, Dirk

AU - Wunderlich, Gerd

AU - Conrad, Karsten

AU - Kotzerke, Joerg

PY - 2012

Y1 - 2012

N2 - Purpose: Assessment of phosphorylated histone H2AX (?H2AX) foci as a measure for double-strand breaks (DSB) is a common technique. Since visual interpretation is time-consuming and influenced by subjective factors, we adapted the pattern recognition algorithms of autoantibodies to automated reading of ?H2AX foci. Materials and methods: DSB formation was assessed by detection of ?H2AX foci after exposition of thyreocyte rat cell line to (188)Re. We used pattern recognition algorithms of the automated fluorescence interpretation system AKLIDES(®) for evaluation of ?H2AX foci. Manual investigation was performed by three laboratories involving five observers. The results were compared by determining correlation and inter-laboratory variability. Results: The study confirmed the adaptation of automated interpretation system AKLIDES® to automated assessment of ?H2AX foci in irradiated cells. Both manual and automated quantification resulted in increasing focus numbers depending on dose. Comparison of automated reading with visual assessment for five manual observers resulted in a determination coefficient of R(2) =?0.889. The inter-laboratory variability for five manual investigators of three laboratories was 38.4 %. Conclusion: The interpretation system AKLIDES(®) demonstrated a high correlation with visually observed results. High inter-laboratory variability found for manual investigations revealed the usefulness for a standardized technique for evaluation of ?H2AX foci.

AB - Purpose: Assessment of phosphorylated histone H2AX (?H2AX) foci as a measure for double-strand breaks (DSB) is a common technique. Since visual interpretation is time-consuming and influenced by subjective factors, we adapted the pattern recognition algorithms of autoantibodies to automated reading of ?H2AX foci. Materials and methods: DSB formation was assessed by detection of ?H2AX foci after exposition of thyreocyte rat cell line to (188)Re. We used pattern recognition algorithms of the automated fluorescence interpretation system AKLIDES(®) for evaluation of ?H2AX foci. Manual investigation was performed by three laboratories involving five observers. The results were compared by determining correlation and inter-laboratory variability. Results: The study confirmed the adaptation of automated interpretation system AKLIDES® to automated assessment of ?H2AX foci in irradiated cells. Both manual and automated quantification resulted in increasing focus numbers depending on dose. Comparison of automated reading with visual assessment for five manual observers resulted in a determination coefficient of R(2) =?0.889. The inter-laboratory variability for five manual investigators of three laboratories was 38.4 %. Conclusion: The interpretation system AKLIDES(®) demonstrated a high correlation with visually observed results. High inter-laboratory variability found for manual investigations revealed the usefulness for a standardized technique for evaluation of ?H2AX foci.

M3 - SCORING: Journal article

VL - 88

SP - 439

EP - 447

JO - INT J RADIAT BIOL

JF - INT J RADIAT BIOL

SN - 0955-3002

IS - 5

M1 - 5

ER -