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FRET Microscopy for Real-Time Visualization of Second Messengers in Living Cells

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FRET Microscopy for Real-Time Visualization of Second Messengers in Living Cells. / Kraft, Axel E; Nikolaev, Viacheslav O.

In: Methods Mol Biol, Vol. 1563, 2017, p. 85-90.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Kraft, AE & Nikolaev, VO 2017, 'FRET Microscopy for Real-Time Visualization of Second Messengers in Living Cells', Methods Mol Biol, vol. 1563, pp. 85-90. https://doi.org/10.1007/978-1-4939-6810-7_6

APA

Kraft, A. E., & Nikolaev, V. O. (2017). FRET Microscopy for Real-Time Visualization of Second Messengers in Living Cells. Methods Mol Biol, 1563, 85-90. https://doi.org/10.1007/978-1-4939-6810-7_6

Vancouver

Kraft AE, Nikolaev VO. FRET Microscopy for Real-Time Visualization of Second Messengers in Living Cells. Methods Mol Biol. 2017;1563:85-90. https://doi.org/10.1007/978-1-4939-6810-7_6

Bibtex

@article{a3c40fa6995f4e3aa8c30a471927ce8b,
title = "FRET Microscopy for Real-Time Visualization of Second Messengers in Living Cells",
abstract = "F{\"o}rster Resonance Energy Transfer (FRET) microscopy is a useful tool in molecular biology and medical research to monitor and quantify real-time dynamics of protein-protein interactions and biochemical processes. Using this well-established technique, many novel signaling mechanisms can be investigated in intact cells or tissues and even in various subcellular compartments. Here, we describe how to perform FRET measurements in living cells expressing FRET-based biosensors and how to evaluate these data. This general protocol can be applied for FRET measurements with various fluorescent biosensors.",
keywords = "Journal Article",
author = "Kraft, {Axel E} and Nikolaev, {Viacheslav O}",
year = "2017",
doi = "10.1007/978-1-4939-6810-7_6",
language = "English",
volume = "1563",
pages = "85--90",
journal = "Methods Mol Biol",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - FRET Microscopy for Real-Time Visualization of Second Messengers in Living Cells

AU - Kraft, Axel E

AU - Nikolaev, Viacheslav O

PY - 2017

Y1 - 2017

N2 - Förster Resonance Energy Transfer (FRET) microscopy is a useful tool in molecular biology and medical research to monitor and quantify real-time dynamics of protein-protein interactions and biochemical processes. Using this well-established technique, many novel signaling mechanisms can be investigated in intact cells or tissues and even in various subcellular compartments. Here, we describe how to perform FRET measurements in living cells expressing FRET-based biosensors and how to evaluate these data. This general protocol can be applied for FRET measurements with various fluorescent biosensors.

AB - Förster Resonance Energy Transfer (FRET) microscopy is a useful tool in molecular biology and medical research to monitor and quantify real-time dynamics of protein-protein interactions and biochemical processes. Using this well-established technique, many novel signaling mechanisms can be investigated in intact cells or tissues and even in various subcellular compartments. Here, we describe how to perform FRET measurements in living cells expressing FRET-based biosensors and how to evaluate these data. This general protocol can be applied for FRET measurements with various fluorescent biosensors.

KW - Journal Article

U2 - 10.1007/978-1-4939-6810-7_6

DO - 10.1007/978-1-4939-6810-7_6

M3 - SCORING: Journal article

C2 - 28324603

VL - 1563

SP - 85

EP - 90

JO - Methods Mol Biol

JF - Methods Mol Biol

SN - 1064-3745

ER -