Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigens

Leonie M Weskamm; Christine Dahlke; Marylyn M Addo

doi:10.1016/j.xpro.2022.101902

Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigens

Standard

Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigens. / Weskamm, Leonie M; Dahlke, Christine; Addo, Marylyn M.

In: STAR PROTOC, Vol. 3, No. 4, 101902, 16.12.2022.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Weskamm, LM, Dahlke, C & Addo, MM 2022, 'Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigens', STAR PROTOC, vol. 3, no. 4, 101902. https://doi.org/10.1016/j.xpro.2022.101902

APA

Weskamm, L. M., Dahlke, C., & Addo, M. M. (2022). Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigens. STAR PROTOC, 3(4), [101902]. https://doi.org/10.1016/j.xpro.2022.101902

Vancouver

Weskamm LM, Dahlke C, Addo MM. Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigens. STAR PROTOC. 2022 Dec 16;3(4). 101902. https://doi.org/10.1016/j.xpro.2022.101902

Bibtex

@article{cc566215c68848b8a82dcb703aa02874,

title = "Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigens",

abstract = "Memory B cells (MBCs), part of the immune response elicited by infection or vaccination, can persist in lymphoid organs and peripheral blood and are capable of rapid reactivation upon secondary antigen exposure. Here, we describe a flow cytometric assay to identify antigen-specific MBCs from peripheral blood mononuclear cells and characterize their isotypes and activation status. We detail steps to use fluorescently labeled antigen probes derived from the SARS-CoV-2 spike protein. These can be adapted to detect MBCs against other antigens. For complete details on the use and execution of this protocol, please refer to Weskamm et al. (2022).1.",

keywords = "Humans, Spike Glycoprotein, Coronavirus, Leukocytes, Mononuclear, Memory B Cells, COVID-19, SARS-CoV-2",

author = "Weskamm, {Leonie M} and Christine Dahlke and Addo, {Marylyn M}",

year = "2022",

month = dec,

day = "16",

doi = "10.1016/j.xpro.2022.101902",

language = "English",

volume = "3",

journal = "STAR PROTOC",

issn = "2666-1667",

publisher = "Cell Press",

number = "4",

}

RIS

TY - JOUR

T1 - Flow cytometric protocol to characterize human memory B cells directed against SARS-CoV-2 spike protein antigens

AU - Weskamm, Leonie M

AU - Dahlke, Christine

AU - Addo, Marylyn M

PY - 2022/12/16

Y1 - 2022/12/16

N2 - Memory B cells (MBCs), part of the immune response elicited by infection or vaccination, can persist in lymphoid organs and peripheral blood and are capable of rapid reactivation upon secondary antigen exposure. Here, we describe a flow cytometric assay to identify antigen-specific MBCs from peripheral blood mononuclear cells and characterize their isotypes and activation status. We detail steps to use fluorescently labeled antigen probes derived from the SARS-CoV-2 spike protein. These can be adapted to detect MBCs against other antigens. For complete details on the use and execution of this protocol, please refer to Weskamm et al. (2022).1.

AB - Memory B cells (MBCs), part of the immune response elicited by infection or vaccination, can persist in lymphoid organs and peripheral blood and are capable of rapid reactivation upon secondary antigen exposure. Here, we describe a flow cytometric assay to identify antigen-specific MBCs from peripheral blood mononuclear cells and characterize their isotypes and activation status. We detail steps to use fluorescently labeled antigen probes derived from the SARS-CoV-2 spike protein. These can be adapted to detect MBCs against other antigens. For complete details on the use and execution of this protocol, please refer to Weskamm et al. (2022).1.

KW - Humans

KW - Spike Glycoprotein, Coronavirus

KW - Leukocytes, Mononuclear

KW - Memory B Cells

KW - COVID-19

KW - SARS-CoV-2

U2 - 10.1016/j.xpro.2022.101902

DO - 10.1016/j.xpro.2022.101902

M3 - SCORING: Journal article

C2 - 36595922

VL - 3

JO - STAR PROTOC

JF - STAR PROTOC

SN - 2666-1667

IS - 4

M1 - 101902

ER -