Fibroblast growth factor receptor 3 is highly expressed in rarely dividing human type A spermatogonia.
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Fibroblast growth factor receptor 3 is highly expressed in rarely dividing human type A spermatogonia. / von Kopylow, Kathrein; Staege, Hannah; Schulze, Wolfgang; Will, Hans; Kirchhoff, Christiane.
In: HISTOCHEM CELL BIOL, Vol. 138, No. 5, 5, 2012, p. 759-772.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Fibroblast growth factor receptor 3 is highly expressed in rarely dividing human type A spermatogonia.
AU - von Kopylow, Kathrein
AU - Staege, Hannah
AU - Schulze, Wolfgang
AU - Will, Hans
AU - Kirchhoff, Christiane
PY - 2012
Y1 - 2012
N2 - Human spermatogonia (Spg) and their fetal precursors express fibroblast growth factor receptor 3 (FGFR3). To further elucidate the role of FGFR3 in the control of Spg self-renewal, proliferation, and/or differentiation, and to narrow down the FGFR3-positive cell type(s) in the normal adult human testis, tissue sections and whole mount preparations of seminiferous tubules were analyzed combining immunofluorescence and confocal fluorescence microscopy. FGFR3 protein was chiefly observed in cellular membranes and cytoplasmic vesicles of a subpopulation of type A Spg, which comprised the chromatin rarefaction zone-containing type A(dark). Cytoplasmic expression of FGFR3 and nuclear expression of proliferation-associated antigen KI-67 were mutually exclusive. Similarly, FGFR3-positive Spg were negative for Doublesex and Mab-3 related transcription factor 1 (DMRT1). By contrast, undifferentiated embryonic cell transcription factor 1 (UTF1) and survival time-associated PHD finger in ovarian cancer 1 protein (SPOC1) were co-expressed in the nuclei of FGFR3-positive Spg. Whole mounted seminiferous tubules illustrated the clonogenic arrangement of the FGFR3/UTF1 double-positive Spg, which mainly occurred as pairs or quadruplets and, different from the KIT-positive Spg, showed no overlap with KI-67 labeled clusters. Taken together, in the adult human testis, FGFR3 expression is a feature of small clones of rarely dividing type A Spg which resemble "undifferentiated" Spg, including the spermatogonial stem cells.
AB - Human spermatogonia (Spg) and their fetal precursors express fibroblast growth factor receptor 3 (FGFR3). To further elucidate the role of FGFR3 in the control of Spg self-renewal, proliferation, and/or differentiation, and to narrow down the FGFR3-positive cell type(s) in the normal adult human testis, tissue sections and whole mount preparations of seminiferous tubules were analyzed combining immunofluorescence and confocal fluorescence microscopy. FGFR3 protein was chiefly observed in cellular membranes and cytoplasmic vesicles of a subpopulation of type A Spg, which comprised the chromatin rarefaction zone-containing type A(dark). Cytoplasmic expression of FGFR3 and nuclear expression of proliferation-associated antigen KI-67 were mutually exclusive. Similarly, FGFR3-positive Spg were negative for Doublesex and Mab-3 related transcription factor 1 (DMRT1). By contrast, undifferentiated embryonic cell transcription factor 1 (UTF1) and survival time-associated PHD finger in ovarian cancer 1 protein (SPOC1) were co-expressed in the nuclei of FGFR3-positive Spg. Whole mounted seminiferous tubules illustrated the clonogenic arrangement of the FGFR3/UTF1 double-positive Spg, which mainly occurred as pairs or quadruplets and, different from the KIT-positive Spg, showed no overlap with KI-67 labeled clusters. Taken together, in the adult human testis, FGFR3 expression is a feature of small clones of rarely dividing type A Spg which resemble "undifferentiated" Spg, including the spermatogonial stem cells.
KW - Humans
KW - Male
KW - Fluorescent Antibody Technique
KW - Microscopy, Confocal
KW - Cell Membrane/metabolism
KW - Testis/cytology/metabolism
KW - Cell Division
KW - Cytoplasmic Vesicles/metabolism
KW - DNA-Binding Proteins/biosynthesis
KW - Ki-67 Antigen/biosynthesis
KW - Nuclear Proteins/biosynthesis
KW - Proto-Oncogene Proteins c-kit/analysis
KW - Receptor, Fibroblast Growth Factor, Type 3/analysis/biosynthesis
KW - Seminiferous Tubules/cytology/metabolism
KW - Spermatogenesis/physiology
KW - Spermatogonia/metabolism
KW - Trans-Activators/biosynthesis
KW - Transcription Factors/analysis/biosynthesis
KW - Humans
KW - Male
KW - Fluorescent Antibody Technique
KW - Microscopy, Confocal
KW - Cell Membrane/metabolism
KW - Testis/cytology/metabolism
KW - Cell Division
KW - Cytoplasmic Vesicles/metabolism
KW - DNA-Binding Proteins/biosynthesis
KW - Ki-67 Antigen/biosynthesis
KW - Nuclear Proteins/biosynthesis
KW - Proto-Oncogene Proteins c-kit/analysis
KW - Receptor, Fibroblast Growth Factor, Type 3/analysis/biosynthesis
KW - Seminiferous Tubules/cytology/metabolism
KW - Spermatogenesis/physiology
KW - Spermatogonia/metabolism
KW - Trans-Activators/biosynthesis
KW - Transcription Factors/analysis/biosynthesis
M3 - SCORING: Journal article
VL - 138
SP - 759
EP - 772
JO - HISTOCHEM CELL BIOL
JF - HISTOCHEM CELL BIOL
SN - 0948-6143
IS - 5
M1 - 5
ER -
