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Fetuin-B, a liver-derived plasma protein is essential for fertilization

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Fetuin-B, a liver-derived plasma protein is essential for fertilization. / Dietzel, Eileen; Wessling, Jennifer; Floehr, Julia; Schäfer, Cora; Ensslen, Silke; Denecke, Bernd; Rösing, Benjamin; Neulen, Joseph; Veitinger, Thomas; Spehr, Marc; Tropartz, Tanja; Tolba, René; Renné, Thomas; Egert, Angela; Schorle, Hubert; Gottenbusch, Yuliya; Hildebrand, André; Yiallouros, Irene; Stöcker, Walter; Weiskirchen, Ralf; Jahnen-Dechent, Willi.

In: DEV CELL, Vol. 25, No. 1, 15.04.2013, p. 106-12.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Dietzel, E, Wessling, J, Floehr, J, Schäfer, C, Ensslen, S, Denecke, B, Rösing, B, Neulen, J, Veitinger, T, Spehr, M, Tropartz, T, Tolba, R, Renné, T, Egert, A, Schorle, H, Gottenbusch, Y, Hildebrand, A, Yiallouros, I, Stöcker, W, Weiskirchen, R & Jahnen-Dechent, W 2013, 'Fetuin-B, a liver-derived plasma protein is essential for fertilization', DEV CELL, vol. 25, no. 1, pp. 106-12. https://doi.org/10.1016/j.devcel.2013.03.001

APA

Dietzel, E., Wessling, J., Floehr, J., Schäfer, C., Ensslen, S., Denecke, B., Rösing, B., Neulen, J., Veitinger, T., Spehr, M., Tropartz, T., Tolba, R., Renné, T., Egert, A., Schorle, H., Gottenbusch, Y., Hildebrand, A., Yiallouros, I., Stöcker, W., ... Jahnen-Dechent, W. (2013). Fetuin-B, a liver-derived plasma protein is essential for fertilization. DEV CELL, 25(1), 106-12. https://doi.org/10.1016/j.devcel.2013.03.001

Vancouver

Dietzel E, Wessling J, Floehr J, Schäfer C, Ensslen S, Denecke B et al. Fetuin-B, a liver-derived plasma protein is essential for fertilization. DEV CELL. 2013 Apr 15;25(1):106-12. https://doi.org/10.1016/j.devcel.2013.03.001

Bibtex

@article{0af5dcabfd804723ac0b09374c9d5d8a,
title = "Fetuin-B, a liver-derived plasma protein is essential for fertilization",
abstract = "The zona pellucida (ZP) is a glycoprotein matrix surrounding mammalian oocytes. Upon fertilization, ZP hardening prevents sperm from binding to and penetrating the ZP. Here, we report that targeted gene deletion of the liver-derived plasma protein fetuin-B causes premature ZP hardening and, consequently, female infertility. Transplanting fetuin-B-deficient ovaries into wild-type recipients restores fertility, indicating that plasma fetuin-B is necessary and sufficient for fertilization. In vitro fertilization of oocytes from fetuin-B-deficient mice only worked after rendering the ZP penetrable by laser perforation. Mechanistically, fetuin-B sustains fertility by inhibiting ovastacin, a cortical granula protease known to trigger ZP hardening. Thus, plasma fetuin-B is necessary to restrain protease activity and thereby maintain ZP permeability until after gamete fusion. These results also show that premature ZP hardening can cause infertility in mice.",
keywords = "Animals, Cell Membrane Permeability, Embryo Transfer, Embryo, Mammalian, Enzyme Activation, Female, Fertilization, Fertilization in Vitro, Fetuin-B, Gene Expression Regulation, Developmental, Infertility, Female, Male, Metalloproteases, Mice, Mice, Inbred C57BL, Oocytes, Ovary, Pregnancy, Recombinant Proteins, Spermatozoa, Zona Pellucida",
author = "Eileen Dietzel and Jennifer Wessling and Julia Floehr and Cora Sch{\"a}fer and Silke Ensslen and Bernd Denecke and Benjamin R{\"o}sing and Joseph Neulen and Thomas Veitinger and Marc Spehr and Tanja Tropartz and Ren{\'e} Tolba and Thomas Renn{\'e} and Angela Egert and Hubert Schorle and Yuliya Gottenbusch and Andr{\'e} Hildebrand and Irene Yiallouros and Walter St{\"o}cker and Ralf Weiskirchen and Willi Jahnen-Dechent",
note = "Copyright {\textcopyright} 2013 Elsevier Inc. All rights reserved.",
year = "2013",
month = apr,
day = "15",
doi = "10.1016/j.devcel.2013.03.001",
language = "English",
volume = "25",
pages = "106--12",
journal = "DEV CELL",
issn = "1534-5807",
publisher = "Cell Press",
number = "1",

}

RIS

TY - JOUR

T1 - Fetuin-B, a liver-derived plasma protein is essential for fertilization

AU - Dietzel, Eileen

AU - Wessling, Jennifer

AU - Floehr, Julia

AU - Schäfer, Cora

AU - Ensslen, Silke

AU - Denecke, Bernd

AU - Rösing, Benjamin

AU - Neulen, Joseph

AU - Veitinger, Thomas

AU - Spehr, Marc

AU - Tropartz, Tanja

AU - Tolba, René

AU - Renné, Thomas

AU - Egert, Angela

AU - Schorle, Hubert

AU - Gottenbusch, Yuliya

AU - Hildebrand, André

AU - Yiallouros, Irene

AU - Stöcker, Walter

AU - Weiskirchen, Ralf

AU - Jahnen-Dechent, Willi

N1 - Copyright © 2013 Elsevier Inc. All rights reserved.

PY - 2013/4/15

Y1 - 2013/4/15

N2 - The zona pellucida (ZP) is a glycoprotein matrix surrounding mammalian oocytes. Upon fertilization, ZP hardening prevents sperm from binding to and penetrating the ZP. Here, we report that targeted gene deletion of the liver-derived plasma protein fetuin-B causes premature ZP hardening and, consequently, female infertility. Transplanting fetuin-B-deficient ovaries into wild-type recipients restores fertility, indicating that plasma fetuin-B is necessary and sufficient for fertilization. In vitro fertilization of oocytes from fetuin-B-deficient mice only worked after rendering the ZP penetrable by laser perforation. Mechanistically, fetuin-B sustains fertility by inhibiting ovastacin, a cortical granula protease known to trigger ZP hardening. Thus, plasma fetuin-B is necessary to restrain protease activity and thereby maintain ZP permeability until after gamete fusion. These results also show that premature ZP hardening can cause infertility in mice.

AB - The zona pellucida (ZP) is a glycoprotein matrix surrounding mammalian oocytes. Upon fertilization, ZP hardening prevents sperm from binding to and penetrating the ZP. Here, we report that targeted gene deletion of the liver-derived plasma protein fetuin-B causes premature ZP hardening and, consequently, female infertility. Transplanting fetuin-B-deficient ovaries into wild-type recipients restores fertility, indicating that plasma fetuin-B is necessary and sufficient for fertilization. In vitro fertilization of oocytes from fetuin-B-deficient mice only worked after rendering the ZP penetrable by laser perforation. Mechanistically, fetuin-B sustains fertility by inhibiting ovastacin, a cortical granula protease known to trigger ZP hardening. Thus, plasma fetuin-B is necessary to restrain protease activity and thereby maintain ZP permeability until after gamete fusion. These results also show that premature ZP hardening can cause infertility in mice.

KW - Animals

KW - Cell Membrane Permeability

KW - Embryo Transfer

KW - Embryo, Mammalian

KW - Enzyme Activation

KW - Female

KW - Fertilization

KW - Fertilization in Vitro

KW - Fetuin-B

KW - Gene Expression Regulation, Developmental

KW - Infertility, Female

KW - Male

KW - Metalloproteases

KW - Mice

KW - Mice, Inbred C57BL

KW - Oocytes

KW - Ovary

KW - Pregnancy

KW - Recombinant Proteins

KW - Spermatozoa

KW - Zona Pellucida

U2 - 10.1016/j.devcel.2013.03.001

DO - 10.1016/j.devcel.2013.03.001

M3 - SCORING: Journal article

C2 - 23562279

VL - 25

SP - 106

EP - 112

JO - DEV CELL

JF - DEV CELL

SN - 1534-5807

IS - 1

ER -