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Extracellular miR-574-5p Induces Osteoclast Differentiation via TLR 7/8 in Rheumatoid Arthritis

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Extracellular miR-574-5p Induces Osteoclast Differentiation via TLR 7/8 in Rheumatoid Arthritis. / Hegewald, Anett B; Breitwieser, Kai; Ottinger, Sarah M; Mobarrez, Fariborz; Korotkova, Marina; Rethi, Bence; Jakobsson, Per-Johan; Catrina, Anca I; Wähämaa, Heidi; Saul, Meike J.

In: FRONT IMMUNOL, Vol. 11, 2020, p. 585282.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Hegewald, AB, Breitwieser, K, Ottinger, SM, Mobarrez, F, Korotkova, M, Rethi, B, Jakobsson, P-J, Catrina, AI, Wähämaa, H & Saul, MJ 2020, 'Extracellular miR-574-5p Induces Osteoclast Differentiation via TLR 7/8 in Rheumatoid Arthritis', FRONT IMMUNOL, vol. 11, pp. 585282. https://doi.org/10.3389/fimmu.2020.585282

APA

Hegewald, A. B., Breitwieser, K., Ottinger, S. M., Mobarrez, F., Korotkova, M., Rethi, B., Jakobsson, P-J., Catrina, A. I., Wähämaa, H., & Saul, M. J. (2020). Extracellular miR-574-5p Induces Osteoclast Differentiation via TLR 7/8 in Rheumatoid Arthritis. FRONT IMMUNOL, 11, 585282. https://doi.org/10.3389/fimmu.2020.585282

Vancouver

Hegewald AB, Breitwieser K, Ottinger SM, Mobarrez F, Korotkova M, Rethi B et al. Extracellular miR-574-5p Induces Osteoclast Differentiation via TLR 7/8 in Rheumatoid Arthritis. FRONT IMMUNOL. 2020;11:585282. https://doi.org/10.3389/fimmu.2020.585282

Bibtex

@article{057365d6c7ad453abcb14b4f8699498e,
title = "Extracellular miR-574-5p Induces Osteoclast Differentiation via TLR 7/8 in Rheumatoid Arthritis",
abstract = "Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by synovial inflammation and joint destruction. Cell-derived small extracellular vesicles (sEV) mediate cell-to-cell communication in the synovial microenvironment by carrying microRNAs (miRs), a class of small non-coding RNAs. Herein, we report that sEV from synovial fluid promote osteoclast differentiation which is attributed to high levels of extracellular miR-574-5p. Moreover, we demonstrate for the first time that enhanced osteoclast maturation is mediated by Toll-like receptor (TLR) 7/8 signaling which is activated by miR-574-5p binding. This is a novel mechanism by which sEV and miRs contribute to RA pathogenesis and indicate that pharmacological inhibition of extracellular miR-574-5p might offer new therapeutic strategies to protect osteoclast-mediated bone destruction in RA.",
keywords = "Arthritis, Rheumatoid/immunology, Cell Differentiation/physiology, Extracellular Vesicles/metabolism, HEK293 Cells, HeLa Cells, Humans, MicroRNAs/metabolism, Osteoclasts/metabolism, Osteogenesis/physiology, Synovial Fluid/metabolism, Toll-Like Receptor 7/metabolism, Toll-Like Receptor 8/metabolism",
author = "Hegewald, {Anett B} and Kai Breitwieser and Ottinger, {Sarah M} and Fariborz Mobarrez and Marina Korotkova and Bence Rethi and Per-Johan Jakobsson and Catrina, {Anca I} and Heidi W{\"a}h{\"a}maa and Saul, {Meike J}",
note = "Copyright {\textcopyright} 2020 Hegewald, Breitwieser, Ottinger, Mobarrez, Korotkova, Rethi, Jakobsson, Catrina, W{\"a}h{\"a}maa and Saul.",
year = "2020",
doi = "10.3389/fimmu.2020.585282",
language = "English",
volume = "11",
pages = "585282",
journal = "FRONT IMMUNOL",
issn = "1664-3224",
publisher = "Lausanne : Frontiers Research Foundation",

}

RIS

TY - JOUR

T1 - Extracellular miR-574-5p Induces Osteoclast Differentiation via TLR 7/8 in Rheumatoid Arthritis

AU - Hegewald, Anett B

AU - Breitwieser, Kai

AU - Ottinger, Sarah M

AU - Mobarrez, Fariborz

AU - Korotkova, Marina

AU - Rethi, Bence

AU - Jakobsson, Per-Johan

AU - Catrina, Anca I

AU - Wähämaa, Heidi

AU - Saul, Meike J

N1 - Copyright © 2020 Hegewald, Breitwieser, Ottinger, Mobarrez, Korotkova, Rethi, Jakobsson, Catrina, Wähämaa and Saul.

PY - 2020

Y1 - 2020

N2 - Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by synovial inflammation and joint destruction. Cell-derived small extracellular vesicles (sEV) mediate cell-to-cell communication in the synovial microenvironment by carrying microRNAs (miRs), a class of small non-coding RNAs. Herein, we report that sEV from synovial fluid promote osteoclast differentiation which is attributed to high levels of extracellular miR-574-5p. Moreover, we demonstrate for the first time that enhanced osteoclast maturation is mediated by Toll-like receptor (TLR) 7/8 signaling which is activated by miR-574-5p binding. This is a novel mechanism by which sEV and miRs contribute to RA pathogenesis and indicate that pharmacological inhibition of extracellular miR-574-5p might offer new therapeutic strategies to protect osteoclast-mediated bone destruction in RA.

AB - Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by synovial inflammation and joint destruction. Cell-derived small extracellular vesicles (sEV) mediate cell-to-cell communication in the synovial microenvironment by carrying microRNAs (miRs), a class of small non-coding RNAs. Herein, we report that sEV from synovial fluid promote osteoclast differentiation which is attributed to high levels of extracellular miR-574-5p. Moreover, we demonstrate for the first time that enhanced osteoclast maturation is mediated by Toll-like receptor (TLR) 7/8 signaling which is activated by miR-574-5p binding. This is a novel mechanism by which sEV and miRs contribute to RA pathogenesis and indicate that pharmacological inhibition of extracellular miR-574-5p might offer new therapeutic strategies to protect osteoclast-mediated bone destruction in RA.

KW - Arthritis, Rheumatoid/immunology

KW - Cell Differentiation/physiology

KW - Extracellular Vesicles/metabolism

KW - HEK293 Cells

KW - HeLa Cells

KW - Humans

KW - MicroRNAs/metabolism

KW - Osteoclasts/metabolism

KW - Osteogenesis/physiology

KW - Synovial Fluid/metabolism

KW - Toll-Like Receptor 7/metabolism

KW - Toll-Like Receptor 8/metabolism

U2 - 10.3389/fimmu.2020.585282

DO - 10.3389/fimmu.2020.585282

M3 - SCORING: Journal article

C2 - 33154755

VL - 11

SP - 585282

JO - FRONT IMMUNOL

JF - FRONT IMMUNOL

SN - 1664-3224

ER -