Research ExplorerPublicationsExtracellular matrix composition and interstitial pH modulat...

Extracellular matrix composition and interstitial pH modulate NHE1-mediated melanoma cell motility

Standard

Extracellular matrix composition and interstitial pH modulate NHE1-mediated melanoma cell motility. / Vahle, Anne-Kristin; Domikowsky, Britta; Schwöppe, Christian; Krähling, Hermann; Mally, Sabine; Schäfers, Michael; Hermann, Sven; Shahin, Victor; Haier, Jörg; Schwab, Albrecht; Stock, Christian.

In: INT J ONCOL, Vol. 44, No. 1, 01.2014, p. 78-90.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Vahle, A-K, Domikowsky, B, Schwöppe, C, Krähling, H, Mally, S, Schäfers, M, Hermann, S, Shahin, V, Haier, J, Schwab, A & Stock, C 2014, 'Extracellular matrix composition and interstitial pH modulate NHE1-mediated melanoma cell motility', INT J ONCOL, vol. 44, no. 1, pp. 78-90. https://doi.org/10.3892/ijo.2013.2158

APA

Vahle, A-K., Domikowsky, B., Schwöppe, C., Krähling, H., Mally, S., Schäfers, M., Hermann, S., Shahin, V., Haier, J., Schwab, A., & Stock, C. (2014). Extracellular matrix composition and interstitial pH modulate NHE1-mediated melanoma cell motility. INT J ONCOL, 44(1), 78-90. https://doi.org/10.3892/ijo.2013.2158

Vancouver

Vahle A-K, Domikowsky B, Schwöppe C, Krähling H, Mally S, Schäfers M et al. Extracellular matrix composition and interstitial pH modulate NHE1-mediated melanoma cell motility. INT J ONCOL. 2014 Jan;44(1):78-90. https://doi.org/10.3892/ijo.2013.2158

Bibtex

@article{eb8f69271c6b4964897605a8aa02aa19,
title = "Extracellular matrix composition and interstitial pH modulate NHE1-mediated melanoma cell motility",
abstract = "The activity of the Na+/H+ exchanger NHE1 is required for human melanoma cell adhesion and migration. The goal of the present study was to suppress mouse melanoma (B16V) cell invasion in vivo by inhibiting NHE1. Intravital observations in mobilized left liver lobes of laparotomized male Sprague-Dawley rats disclosed that five minutes after intra-arterial administration of the B16V cell suspension, cells adhered to the endothelia of liver sinusoidal capillaries and started to migrate into the surrounding liver tissue. In the presence of the NHE1-specific inhibitor cariporide, migration/invasion was reduced by about 50% while adhesion was not lowered. Time-lapse video microscopy and adhesion/invasion assays revealed that in vitro, blockade of NHE1 by cariporide i) significantly decreased the migratory speed of the cells and ii) completely inhibited the invasive behavior of both an artificial, basement membrane-like and a dermis-like matrix. Cells were more motile on the basement membrane and more invasive on the dermis-like matrix. Small-animal PET (positron-emission tomography) analyses of B16V metastasis in female C57BL/6 mice showed that, although NHE1 inhibition hardly affected the percentage of animals developing metastases or relapses, metastases seem to get directed to the lungs in cariporide-treated animals while animals feeding on the standard diet show metastases spread all over the body. We conclude that i) B16V cells prefer to invade a dermis-like rather than a basement membrane-like matrix; ii) the extracellular matrix (ECM) composition strongly impacts on NHE1-dependent in vitro cell motility and invasion; and iii) the lungs are metastasis‑prone and impair the efficiency of cariporide due to their ECM composition and the pulmonary interstitial (extravascular) pH.",
keywords = "Animals, Cation Transport Proteins, Cell Adhesion, Cell Movement, Extracellular Matrix, Female, Guanidines, Humans, Hydrogen-Ion Concentration, Melanoma, Experimental, Mice, Neoplasm Invasiveness, Rats, Sodium-Hydrogen Antiporter, Sulfones",
author = "Anne-Kristin Vahle and Britta Domikowsky and Christian Schw{\"o}ppe and Hermann Kr{\"a}hling and Sabine Mally and Michael Sch{\"a}fers and Sven Hermann and Victor Shahin and J{\"o}rg Haier and Albrecht Schwab and Christian Stock",
year = "2014",
month = jan,
doi = "10.3892/ijo.2013.2158",
language = "English",
volume = "44",
pages = "78--90",
journal = "INT J ONCOL",
issn = "1019-6439",
publisher = "Spandidos Publications",
number = "1",

}

RIS

TY - JOUR

T1 - Extracellular matrix composition and interstitial pH modulate NHE1-mediated melanoma cell motility

AU - Vahle, Anne-Kristin

AU - Domikowsky, Britta

AU - Schwöppe, Christian

AU - Krähling, Hermann

AU - Mally, Sabine

AU - Schäfers, Michael

AU - Hermann, Sven

AU - Shahin, Victor

AU - Haier, Jörg

AU - Schwab, Albrecht

AU - Stock, Christian

PY - 2014/1

Y1 - 2014/1

N2 - The activity of the Na+/H+ exchanger NHE1 is required for human melanoma cell adhesion and migration. The goal of the present study was to suppress mouse melanoma (B16V) cell invasion in vivo by inhibiting NHE1. Intravital observations in mobilized left liver lobes of laparotomized male Sprague-Dawley rats disclosed that five minutes after intra-arterial administration of the B16V cell suspension, cells adhered to the endothelia of liver sinusoidal capillaries and started to migrate into the surrounding liver tissue. In the presence of the NHE1-specific inhibitor cariporide, migration/invasion was reduced by about 50% while adhesion was not lowered. Time-lapse video microscopy and adhesion/invasion assays revealed that in vitro, blockade of NHE1 by cariporide i) significantly decreased the migratory speed of the cells and ii) completely inhibited the invasive behavior of both an artificial, basement membrane-like and a dermis-like matrix. Cells were more motile on the basement membrane and more invasive on the dermis-like matrix. Small-animal PET (positron-emission tomography) analyses of B16V metastasis in female C57BL/6 mice showed that, although NHE1 inhibition hardly affected the percentage of animals developing metastases or relapses, metastases seem to get directed to the lungs in cariporide-treated animals while animals feeding on the standard diet show metastases spread all over the body. We conclude that i) B16V cells prefer to invade a dermis-like rather than a basement membrane-like matrix; ii) the extracellular matrix (ECM) composition strongly impacts on NHE1-dependent in vitro cell motility and invasion; and iii) the lungs are metastasis‑prone and impair the efficiency of cariporide due to their ECM composition and the pulmonary interstitial (extravascular) pH.

AB - The activity of the Na+/H+ exchanger NHE1 is required for human melanoma cell adhesion and migration. The goal of the present study was to suppress mouse melanoma (B16V) cell invasion in vivo by inhibiting NHE1. Intravital observations in mobilized left liver lobes of laparotomized male Sprague-Dawley rats disclosed that five minutes after intra-arterial administration of the B16V cell suspension, cells adhered to the endothelia of liver sinusoidal capillaries and started to migrate into the surrounding liver tissue. In the presence of the NHE1-specific inhibitor cariporide, migration/invasion was reduced by about 50% while adhesion was not lowered. Time-lapse video microscopy and adhesion/invasion assays revealed that in vitro, blockade of NHE1 by cariporide i) significantly decreased the migratory speed of the cells and ii) completely inhibited the invasive behavior of both an artificial, basement membrane-like and a dermis-like matrix. Cells were more motile on the basement membrane and more invasive on the dermis-like matrix. Small-animal PET (positron-emission tomography) analyses of B16V metastasis in female C57BL/6 mice showed that, although NHE1 inhibition hardly affected the percentage of animals developing metastases or relapses, metastases seem to get directed to the lungs in cariporide-treated animals while animals feeding on the standard diet show metastases spread all over the body. We conclude that i) B16V cells prefer to invade a dermis-like rather than a basement membrane-like matrix; ii) the extracellular matrix (ECM) composition strongly impacts on NHE1-dependent in vitro cell motility and invasion; and iii) the lungs are metastasis‑prone and impair the efficiency of cariporide due to their ECM composition and the pulmonary interstitial (extravascular) pH.

KW - Animals

KW - Cation Transport Proteins

KW - Cell Adhesion

KW - Cell Movement

KW - Extracellular Matrix

KW - Female

KW - Guanidines

KW - Humans

KW - Hydrogen-Ion Concentration

KW - Melanoma, Experimental

KW - Mice

KW - Neoplasm Invasiveness

KW - Rats

KW - Sodium-Hydrogen Antiporter

KW - Sulfones

U2 - 10.3892/ijo.2013.2158

DO - 10.3892/ijo.2013.2158

M3 - SCORING: Journal article

C2 - 24173371

VL - 44

SP - 78

EP - 90

JO - INT J ONCOL

JF - INT J ONCOL

SN - 1019-6439

IS - 1

ER -