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Expression of VEGFR3 in glioma endothelium correlates with tumor grade

Standard

Expression of VEGFR3 in glioma endothelium correlates with tumor grade. / Grau, S J; Trillsch, F; Herms, J; Thon, N; Nelson, P J; Tonn, J-C; Goldbrunner, R.

In: J NEURO-ONCOL, Vol. 82, No. 2, 01.04.2007, p. 141-50.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Grau, SJ, Trillsch, F, Herms, J, Thon, N, Nelson, PJ, Tonn, J-C & Goldbrunner, R 2007, 'Expression of VEGFR3 in glioma endothelium correlates with tumor grade', J NEURO-ONCOL, vol. 82, no. 2, pp. 141-50. https://doi.org/10.1007/s11060-006-9272-4

APA

Grau, S. J., Trillsch, F., Herms, J., Thon, N., Nelson, P. J., Tonn, J-C., & Goldbrunner, R. (2007). Expression of VEGFR3 in glioma endothelium correlates with tumor grade. J NEURO-ONCOL, 82(2), 141-50. https://doi.org/10.1007/s11060-006-9272-4

Vancouver

Grau SJ, Trillsch F, Herms J, Thon N, Nelson PJ, Tonn J-C et al. Expression of VEGFR3 in glioma endothelium correlates with tumor grade. J NEURO-ONCOL. 2007 Apr 1;82(2):141-50. https://doi.org/10.1007/s11060-006-9272-4

Bibtex

@article{dce1f16e443e47e58909319e19ad062b,
title = "Expression of VEGFR3 in glioma endothelium correlates with tumor grade",
abstract = "Angiogenic processes are regulated by vascular endothelial growth factors (VEGFs) and their receptors VEGFR1 (Flt-1), 2 (Flk-1) and 3 (Flt-4). While VEGFR2 is thought to play a central role in tumor angiogenesis, anti-angiogenic therapies targeting VEGFR2 in glioma models can show escape phenomena with secondary onset of angiogenesis. The purpose of this study was to find explanations for these processes by searching for alternative pathways regulating glioma angiogenesis and reveal a correlation with tumor grade. Thus, VEGFR3, which is not expressed in normal brain, and its ligands VEGF-C and -D, were assessed in high grade (WHO degrees IV, glioblastomas, GBM) and low grade gliomas [WHO degrees II astrocytomas (AII)]. In all GBM, a strong protein expression of VEGFR3 was found on tumor endothelium, VEGF-C and -D expression was found on numerous cells in areas of high vascularization. On RNA level, a significant up-regulation of VEGFR3 was detected in GBM compared to AII and non-neoplastic brain. In AII, only very moderate VEGFR3, VEGF-C and -D expression was found on protein and RNA level indicating a correlation of VEGFR3 expression with tumor grade. VEGFR3 signal in both grades was found predominantly on endothelial cells, confirmed by VEGFR3 expression on isolated CD31 positive cells and the expression of various endothelial markers on VEGFR3-positive cells isolated from GBM. The demonstration of a complete angiogenic signaling system that is dependent on tumor grade may influence the traditional paradigm of glioma angiogenesis and may provide a basis for more effective anti-angiogenic treatment strategies.",
keywords = "Adult, Aged, Antigens, CD31, Astrocytoma, Brain Neoplasms, Endothelium, Vascular, Female, Humans, Male, Middle Aged, Neoplasm Staging, Neovascularization, Pathologic, Tumor Cells, Cultured, Vascular Endothelial Growth Factor C, Vascular Endothelial Growth Factor D, Vascular Endothelial Growth Factor Receptor-2, Vascular Endothelial Growth Factor Receptor-3",
author = "Grau, {S J} and F Trillsch and J Herms and N Thon and Nelson, {P J} and J-C Tonn and R Goldbrunner",
year = "2007",
month = apr,
day = "1",
doi = "10.1007/s11060-006-9272-4",
language = "English",
volume = "82",
pages = "141--50",
journal = "J NEURO-ONCOL",
issn = "0167-594X",
publisher = "Kluwer Academic Publishers",
number = "2",

}

RIS

TY - JOUR

T1 - Expression of VEGFR3 in glioma endothelium correlates with tumor grade

AU - Grau, S J

AU - Trillsch, F

AU - Herms, J

AU - Thon, N

AU - Nelson, P J

AU - Tonn, J-C

AU - Goldbrunner, R

PY - 2007/4/1

Y1 - 2007/4/1

N2 - Angiogenic processes are regulated by vascular endothelial growth factors (VEGFs) and their receptors VEGFR1 (Flt-1), 2 (Flk-1) and 3 (Flt-4). While VEGFR2 is thought to play a central role in tumor angiogenesis, anti-angiogenic therapies targeting VEGFR2 in glioma models can show escape phenomena with secondary onset of angiogenesis. The purpose of this study was to find explanations for these processes by searching for alternative pathways regulating glioma angiogenesis and reveal a correlation with tumor grade. Thus, VEGFR3, which is not expressed in normal brain, and its ligands VEGF-C and -D, were assessed in high grade (WHO degrees IV, glioblastomas, GBM) and low grade gliomas [WHO degrees II astrocytomas (AII)]. In all GBM, a strong protein expression of VEGFR3 was found on tumor endothelium, VEGF-C and -D expression was found on numerous cells in areas of high vascularization. On RNA level, a significant up-regulation of VEGFR3 was detected in GBM compared to AII and non-neoplastic brain. In AII, only very moderate VEGFR3, VEGF-C and -D expression was found on protein and RNA level indicating a correlation of VEGFR3 expression with tumor grade. VEGFR3 signal in both grades was found predominantly on endothelial cells, confirmed by VEGFR3 expression on isolated CD31 positive cells and the expression of various endothelial markers on VEGFR3-positive cells isolated from GBM. The demonstration of a complete angiogenic signaling system that is dependent on tumor grade may influence the traditional paradigm of glioma angiogenesis and may provide a basis for more effective anti-angiogenic treatment strategies.

AB - Angiogenic processes are regulated by vascular endothelial growth factors (VEGFs) and their receptors VEGFR1 (Flt-1), 2 (Flk-1) and 3 (Flt-4). While VEGFR2 is thought to play a central role in tumor angiogenesis, anti-angiogenic therapies targeting VEGFR2 in glioma models can show escape phenomena with secondary onset of angiogenesis. The purpose of this study was to find explanations for these processes by searching for alternative pathways regulating glioma angiogenesis and reveal a correlation with tumor grade. Thus, VEGFR3, which is not expressed in normal brain, and its ligands VEGF-C and -D, were assessed in high grade (WHO degrees IV, glioblastomas, GBM) and low grade gliomas [WHO degrees II astrocytomas (AII)]. In all GBM, a strong protein expression of VEGFR3 was found on tumor endothelium, VEGF-C and -D expression was found on numerous cells in areas of high vascularization. On RNA level, a significant up-regulation of VEGFR3 was detected in GBM compared to AII and non-neoplastic brain. In AII, only very moderate VEGFR3, VEGF-C and -D expression was found on protein and RNA level indicating a correlation of VEGFR3 expression with tumor grade. VEGFR3 signal in both grades was found predominantly on endothelial cells, confirmed by VEGFR3 expression on isolated CD31 positive cells and the expression of various endothelial markers on VEGFR3-positive cells isolated from GBM. The demonstration of a complete angiogenic signaling system that is dependent on tumor grade may influence the traditional paradigm of glioma angiogenesis and may provide a basis for more effective anti-angiogenic treatment strategies.

KW - Adult

KW - Aged

KW - Antigens, CD31

KW - Astrocytoma

KW - Brain Neoplasms

KW - Endothelium, Vascular

KW - Female

KW - Humans

KW - Male

KW - Middle Aged

KW - Neoplasm Staging

KW - Neovascularization, Pathologic

KW - Tumor Cells, Cultured

KW - Vascular Endothelial Growth Factor C

KW - Vascular Endothelial Growth Factor D

KW - Vascular Endothelial Growth Factor Receptor-2

KW - Vascular Endothelial Growth Factor Receptor-3

U2 - 10.1007/s11060-006-9272-4

DO - 10.1007/s11060-006-9272-4

M3 - SCORING: Journal article

C2 - 17115285

VL - 82

SP - 141

EP - 150

JO - J NEURO-ONCOL

JF - J NEURO-ONCOL

SN - 0167-594X

IS - 2

ER -