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Expression of tight and adherens junction proteins in ulcerative colitis associated colorectal carcinoma

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Expression of tight and adherens junction proteins in ulcerative colitis associated colorectal carcinoma : upregulation of claudin-1, claudin-3, claudin-4, and beta-catenin. / Mees, S T; Mennigen, R; Spieker, T; Rijcken, E; Senninger, N; Haier, J; Bruewer, M.

In: INT J COLORECTAL DIS, Vol. 24, No. 4, 04.2009, p. 361-8.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Mees, ST, Mennigen, R, Spieker, T, Rijcken, E, Senninger, N, Haier, J & Bruewer, M 2009, 'Expression of tight and adherens junction proteins in ulcerative colitis associated colorectal carcinoma: upregulation of claudin-1, claudin-3, claudin-4, and beta-catenin', INT J COLORECTAL DIS, vol. 24, no. 4, pp. 361-8. https://doi.org/10.1007/s00384-009-0653-y

APA

Mees, S. T., Mennigen, R., Spieker, T., Rijcken, E., Senninger, N., Haier, J., & Bruewer, M. (2009). Expression of tight and adherens junction proteins in ulcerative colitis associated colorectal carcinoma: upregulation of claudin-1, claudin-3, claudin-4, and beta-catenin. INT J COLORECTAL DIS, 24(4), 361-8. https://doi.org/10.1007/s00384-009-0653-y

Vancouver

Mees ST, Mennigen R, Spieker T, Rijcken E, Senninger N, Haier J et al. Expression of tight and adherens junction proteins in ulcerative colitis associated colorectal carcinoma: upregulation of claudin-1, claudin-3, claudin-4, and beta-catenin. INT J COLORECTAL DIS. 2009 Apr;24(4):361-8. https://doi.org/10.1007/s00384-009-0653-y

Bibtex

@article{4a9b7881e05a4097a5ecaae995dea427,
title = "Expression of tight and adherens junction proteins in ulcerative colitis associated colorectal carcinoma: upregulation of claudin-1, claudin-3, claudin-4, and beta-catenin",
abstract = "BACKGROUND: Tight junction (TJ) proteins play a critical role in cellular adhesion, glandular differentiation, and cellular proliferation. The function of these proteins is compromised in a number of intestinal diseases, including ulcerative colitis that has an increased incidence for colorectal carcinoma (CAC). The aim of this study was to determine the expression of TJ proteins, claudin-1-4, occludin, ZO-1, and the adherens junction (AJ) protein beta-catenin in CAC.METHODS: Sixteen colectomy specimens with CAC, adjoining intraepithelial neoplasia, and normal mucosa were studied by immunofluorescence. A semiquantitative evaluation of all investigated proteins was performed by scoring the staining intensity, and the TJ and AJ protein expression in neoplastic cells was compared to normal and intraepithelial neoplastic colonic mucosa.RESULTS: Using an intensity scoring system, mucosa of crypts and surfaces of CAC exhibited significantly elevated expression levels of claudin-1, claudin-3, claudin-4, and beta-catenin compared to intraepithelial neoplasia and normal mucosa (p<0.05). These data were confirmed by a comparative score. The expression of claudin-2, occludin, and ZO-1 showed no differences between the groups.CONCLUSION: TJ proteins claudin-1, claudin-3, claudin-4, and the AJ protein beta-catenin are overexpressed in CAC. This suggests that these proteins may become potential markers and targets in CAC.",
keywords = "Adherens Junctions, Adult, Aged, Carcinoma in Situ, Claudin-1, Claudin-3, Claudin-4, Colitis, Ulcerative, Colorectal Neoplasms, Demography, Female, Fluorescent Antibody Technique, Humans, Intestinal Mucosa, Male, Membrane Proteins, Middle Aged, Tight Junctions, Up-Regulation, beta Catenin",
author = "Mees, {S T} and R Mennigen and T Spieker and E Rijcken and N Senninger and J Haier and M Bruewer",
year = "2009",
month = apr,
doi = "10.1007/s00384-009-0653-y",
language = "English",
volume = "24",
pages = "361--8",
journal = "INT J COLORECTAL DIS",
issn = "0179-1958",
publisher = "Springer",
number = "4",

}

RIS

TY - JOUR

T1 - Expression of tight and adherens junction proteins in ulcerative colitis associated colorectal carcinoma

T2 - upregulation of claudin-1, claudin-3, claudin-4, and beta-catenin

AU - Mees, S T

AU - Mennigen, R

AU - Spieker, T

AU - Rijcken, E

AU - Senninger, N

AU - Haier, J

AU - Bruewer, M

PY - 2009/4

Y1 - 2009/4

N2 - BACKGROUND: Tight junction (TJ) proteins play a critical role in cellular adhesion, glandular differentiation, and cellular proliferation. The function of these proteins is compromised in a number of intestinal diseases, including ulcerative colitis that has an increased incidence for colorectal carcinoma (CAC). The aim of this study was to determine the expression of TJ proteins, claudin-1-4, occludin, ZO-1, and the adherens junction (AJ) protein beta-catenin in CAC.METHODS: Sixteen colectomy specimens with CAC, adjoining intraepithelial neoplasia, and normal mucosa were studied by immunofluorescence. A semiquantitative evaluation of all investigated proteins was performed by scoring the staining intensity, and the TJ and AJ protein expression in neoplastic cells was compared to normal and intraepithelial neoplastic colonic mucosa.RESULTS: Using an intensity scoring system, mucosa of crypts and surfaces of CAC exhibited significantly elevated expression levels of claudin-1, claudin-3, claudin-4, and beta-catenin compared to intraepithelial neoplasia and normal mucosa (p<0.05). These data were confirmed by a comparative score. The expression of claudin-2, occludin, and ZO-1 showed no differences between the groups.CONCLUSION: TJ proteins claudin-1, claudin-3, claudin-4, and the AJ protein beta-catenin are overexpressed in CAC. This suggests that these proteins may become potential markers and targets in CAC.

AB - BACKGROUND: Tight junction (TJ) proteins play a critical role in cellular adhesion, glandular differentiation, and cellular proliferation. The function of these proteins is compromised in a number of intestinal diseases, including ulcerative colitis that has an increased incidence for colorectal carcinoma (CAC). The aim of this study was to determine the expression of TJ proteins, claudin-1-4, occludin, ZO-1, and the adherens junction (AJ) protein beta-catenin in CAC.METHODS: Sixteen colectomy specimens with CAC, adjoining intraepithelial neoplasia, and normal mucosa were studied by immunofluorescence. A semiquantitative evaluation of all investigated proteins was performed by scoring the staining intensity, and the TJ and AJ protein expression in neoplastic cells was compared to normal and intraepithelial neoplastic colonic mucosa.RESULTS: Using an intensity scoring system, mucosa of crypts and surfaces of CAC exhibited significantly elevated expression levels of claudin-1, claudin-3, claudin-4, and beta-catenin compared to intraepithelial neoplasia and normal mucosa (p<0.05). These data were confirmed by a comparative score. The expression of claudin-2, occludin, and ZO-1 showed no differences between the groups.CONCLUSION: TJ proteins claudin-1, claudin-3, claudin-4, and the AJ protein beta-catenin are overexpressed in CAC. This suggests that these proteins may become potential markers and targets in CAC.

KW - Adherens Junctions

KW - Adult

KW - Aged

KW - Carcinoma in Situ

KW - Claudin-1

KW - Claudin-3

KW - Claudin-4

KW - Colitis, Ulcerative

KW - Colorectal Neoplasms

KW - Demography

KW - Female

KW - Fluorescent Antibody Technique

KW - Humans

KW - Intestinal Mucosa

KW - Male

KW - Membrane Proteins

KW - Middle Aged

KW - Tight Junctions

KW - Up-Regulation

KW - beta Catenin

U2 - 10.1007/s00384-009-0653-y

DO - 10.1007/s00384-009-0653-y

M3 - SCORING: Journal article

C2 - 19184060

VL - 24

SP - 361

EP - 368

JO - INT J COLORECTAL DIS

JF - INT J COLORECTAL DIS

SN - 0179-1958

IS - 4

ER -