Expression of the MCP-1 gene and the HPV 16 E6/E7 oncogenes in squamous cell carcinomas of the cervix uteri and metastases.
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Expression of the MCP-1 gene and the HPV 16 E6/E7 oncogenes in squamous cell carcinomas of the cervix uteri and metastases. / Riethdorf, Sabine; Riethdorf, L; Richter, N; Löning, Thomas.
In: PATHOBIOLOGY, Vol. 66, No. 6, 6, 1998, p. 260-267.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Expression of the MCP-1 gene and the HPV 16 E6/E7 oncogenes in squamous cell carcinomas of the cervix uteri and metastases.
AU - Riethdorf, Sabine
AU - Riethdorf, L
AU - Richter, N
AU - Löning, Thomas
PY - 1998
Y1 - 1998
N2 - Monocyte chemoattractant protein 1 (MCP-1) plays an important role in the recruitment of monocytes in solid tumors. Previously, for cervical carcinoma cell lines an inverse correlation between the expression of the MCP-1 gene and the human papillomavirus (HPV) oncogenes E6/E7 was described. In this study paraffin-embedded biopsy specimens from 25 squamous cell carcinomas of the cervix uteri were analyzed for the expression of the MCP-1 gene and the HPV 16 E6/E7 oncogenes by RNA/RNA in situ hybridization. Similar to in vitro analyses, in our material a negative correlation between the transcription of these genes in tumor cells could be demonstrated in 19/25 cases, whereas in 6 cases both MCP-1 gene and HPV oncogene transcription could be detected. Interestingly, the same results regarding MCP-1 gene and HPV oncogene expression were observed in the majority of corresponding metastases. On the other hand, in stromal cells MCP-1-specific transcripts could be detected in all cases. In most of them intra- and/or peritumoral macrophages were observed. Our findings support the hypothesis that HPV oncoproteins are negative regulators of MCP-1 transcription.
AB - Monocyte chemoattractant protein 1 (MCP-1) plays an important role in the recruitment of monocytes in solid tumors. Previously, for cervical carcinoma cell lines an inverse correlation between the expression of the MCP-1 gene and the human papillomavirus (HPV) oncogenes E6/E7 was described. In this study paraffin-embedded biopsy specimens from 25 squamous cell carcinomas of the cervix uteri were analyzed for the expression of the MCP-1 gene and the HPV 16 E6/E7 oncogenes by RNA/RNA in situ hybridization. Similar to in vitro analyses, in our material a negative correlation between the transcription of these genes in tumor cells could be demonstrated in 19/25 cases, whereas in 6 cases both MCP-1 gene and HPV oncogene transcription could be detected. Interestingly, the same results regarding MCP-1 gene and HPV oncogene expression were observed in the majority of corresponding metastases. On the other hand, in stromal cells MCP-1-specific transcripts could be detected in all cases. In most of them intra- and/or peritumoral macrophages were observed. Our findings support the hypothesis that HPV oncoproteins are negative regulators of MCP-1 transcription.
M3 - SCORING: Zeitschriftenaufsatz
VL - 66
SP - 260
EP - 267
JO - PATHOBIOLOGY
JF - PATHOBIOLOGY
SN - 1015-2008
IS - 6
M1 - 6
ER -
