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Expression of functional CCR and CXCR chemokine receptors in podocytes

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Expression of functional CCR and CXCR chemokine receptors in podocytes. / Huber, Tobias Bruno; Reinhardt, Hans Christian; Exner, Markus; Burger, Jan Andreas; Kerjaschki, Dontscho; Saleem, Moin A; Pavenstädt, Hermann.

In: J IMMUNOL, Vol. 168, No. 12, 15.06.2002, p. 6244-52.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Huber, TB, Reinhardt, HC, Exner, M, Burger, JA, Kerjaschki, D, Saleem, MA & Pavenstädt, H 2002, 'Expression of functional CCR and CXCR chemokine receptors in podocytes', J IMMUNOL, vol. 168, no. 12, pp. 6244-52.

APA

Huber, T. B., Reinhardt, H. C., Exner, M., Burger, J. A., Kerjaschki, D., Saleem, M. A., & Pavenstädt, H. (2002). Expression of functional CCR and CXCR chemokine receptors in podocytes. J IMMUNOL, 168(12), 6244-52.

Vancouver

Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA et al. Expression of functional CCR and CXCR chemokine receptors in podocytes. J IMMUNOL. 2002 Jun 15;168(12):6244-52.

Bibtex

@article{9d95bf83506f4b5785554eac906f943f,
title = "Expression of functional CCR and CXCR chemokine receptors in podocytes",
abstract = "Chemokines and their receptors play an important role in the pathogenesis of acute and chronic glomerular inflammation. However, their expression pattern and function in glomerular podocytes, the primary target cells in a variety of glomerulopathies, have not been investigated as of yet. Using RT-PCR, we now demonstrate the expression of CCR4, CCR8, CCR9, CCR10, CXCR1, CXCR3, CXCR4, and CXCR5 in cultured human podocytes. Stimulation of these receptors induced a concentration-dependent biphasic increase of the free cytosolic calcium concentration in podocytes in culture. In addition, we demonstrate that podocytes release IL-8 in the presence of FCS and that IL-8 down-regulates cell surface CXCR1. Chemokine stimulation of the detected CCRs and CXCRs increased activity of NADPH-oxidase, the primary source of superoxide anions in podocytes. Immunohistochemistry studies revealed only diffuse and weak CXCR expression in healthy human glomerula. In contrast, in membranous nephropathy, a characteristic podocyte disorder, the expression of CXCR1, CXCR3, and CXCR5 is up-regulated in podocytes. In conclusion, podocytes in culture and podocytes in human kidney sections express a set of chemokine receptors. The release of oxygen radicals that accompanies the activation of CCRs and CXCRs may contribute to podocyte injury and the development of proteinuria during membranous nephropathy.",
keywords = "Calcium, Cations, Divalent, Cell Line, Transformed, Cells, Cultured, Cytosol, Fluorescent Antibody Technique, Glomerulonephritis, Membranous, Humans, Immunohistochemistry, Interleukin-8, Kidney Glomerulus, Ligands, RNA, Messenger, Receptors, CXCR3, Receptors, CXCR4, Receptors, CXCR5, Receptors, Chemokine, Receptors, Cytokine, Receptors, Interleukin-8A, Receptors, Interleukin-8B, Serum Albumin, Bovine, Superoxides, Journal Article, Research Support, Non-U.S. Gov't",
author = "Huber, {Tobias Bruno} and Reinhardt, {Hans Christian} and Markus Exner and Burger, {Jan Andreas} and Dontscho Kerjaschki and Saleem, {Moin A} and Hermann Pavenst{\"a}dt",
year = "2002",
month = jun,
day = "15",
language = "English",
volume = "168",
pages = "6244--52",
journal = "J IMMUNOL",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "12",

}

RIS

TY - JOUR

T1 - Expression of functional CCR and CXCR chemokine receptors in podocytes

AU - Huber, Tobias Bruno

AU - Reinhardt, Hans Christian

AU - Exner, Markus

AU - Burger, Jan Andreas

AU - Kerjaschki, Dontscho

AU - Saleem, Moin A

AU - Pavenstädt, Hermann

PY - 2002/6/15

Y1 - 2002/6/15

N2 - Chemokines and their receptors play an important role in the pathogenesis of acute and chronic glomerular inflammation. However, their expression pattern and function in glomerular podocytes, the primary target cells in a variety of glomerulopathies, have not been investigated as of yet. Using RT-PCR, we now demonstrate the expression of CCR4, CCR8, CCR9, CCR10, CXCR1, CXCR3, CXCR4, and CXCR5 in cultured human podocytes. Stimulation of these receptors induced a concentration-dependent biphasic increase of the free cytosolic calcium concentration in podocytes in culture. In addition, we demonstrate that podocytes release IL-8 in the presence of FCS and that IL-8 down-regulates cell surface CXCR1. Chemokine stimulation of the detected CCRs and CXCRs increased activity of NADPH-oxidase, the primary source of superoxide anions in podocytes. Immunohistochemistry studies revealed only diffuse and weak CXCR expression in healthy human glomerula. In contrast, in membranous nephropathy, a characteristic podocyte disorder, the expression of CXCR1, CXCR3, and CXCR5 is up-regulated in podocytes. In conclusion, podocytes in culture and podocytes in human kidney sections express a set of chemokine receptors. The release of oxygen radicals that accompanies the activation of CCRs and CXCRs may contribute to podocyte injury and the development of proteinuria during membranous nephropathy.

AB - Chemokines and their receptors play an important role in the pathogenesis of acute and chronic glomerular inflammation. However, their expression pattern and function in glomerular podocytes, the primary target cells in a variety of glomerulopathies, have not been investigated as of yet. Using RT-PCR, we now demonstrate the expression of CCR4, CCR8, CCR9, CCR10, CXCR1, CXCR3, CXCR4, and CXCR5 in cultured human podocytes. Stimulation of these receptors induced a concentration-dependent biphasic increase of the free cytosolic calcium concentration in podocytes in culture. In addition, we demonstrate that podocytes release IL-8 in the presence of FCS and that IL-8 down-regulates cell surface CXCR1. Chemokine stimulation of the detected CCRs and CXCRs increased activity of NADPH-oxidase, the primary source of superoxide anions in podocytes. Immunohistochemistry studies revealed only diffuse and weak CXCR expression in healthy human glomerula. In contrast, in membranous nephropathy, a characteristic podocyte disorder, the expression of CXCR1, CXCR3, and CXCR5 is up-regulated in podocytes. In conclusion, podocytes in culture and podocytes in human kidney sections express a set of chemokine receptors. The release of oxygen radicals that accompanies the activation of CCRs and CXCRs may contribute to podocyte injury and the development of proteinuria during membranous nephropathy.

KW - Calcium

KW - Cations, Divalent

KW - Cell Line, Transformed

KW - Cells, Cultured

KW - Cytosol

KW - Fluorescent Antibody Technique

KW - Glomerulonephritis, Membranous

KW - Humans

KW - Immunohistochemistry

KW - Interleukin-8

KW - Kidney Glomerulus

KW - Ligands

KW - RNA, Messenger

KW - Receptors, CXCR3

KW - Receptors, CXCR4

KW - Receptors, CXCR5

KW - Receptors, Chemokine

KW - Receptors, Cytokine

KW - Receptors, Interleukin-8A

KW - Receptors, Interleukin-8B

KW - Serum Albumin, Bovine

KW - Superoxides

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - SCORING: Journal article

C2 - 12055238

VL - 168

SP - 6244

EP - 6252

JO - J IMMUNOL

JF - J IMMUNOL

SN - 0022-1767

IS - 12

ER -