Expression of decorin and collagens I and III in different layers of human skin in vivo: a laser capture microdissection study.

Standard

Expression of decorin and collagens I and III in different layers of human skin in vivo: a laser capture microdissection study. / Lochner, Katrin; Gaemlich, Astrid; Südel, Kirstin Marie; Venzke, Kirsten; Moll, Ingrid; Knott, Anja; Stäb, Franz; Wenck, Horst; Döring, Olaf; Böttger, Michael; Gallinat, Stefan.

In: BIOGERONTOLOGY, Vol. 8, No. 3, 3, 2007, p. 269-282.

Research output: SCORING: Contribution to journalSCORING: Journal articleResearchpeer-review

Harvard

Lochner, K, Gaemlich, A, Südel, KM, Venzke, K, Moll, I, Knott, A, Stäb, F, Wenck, H, Döring, O, Böttger, M & Gallinat, S 2007, 'Expression of decorin and collagens I and III in different layers of human skin in vivo: a laser capture microdissection study.', BIOGERONTOLOGY, vol. 8, no. 3, 3, pp. 269-282. <http://www.ncbi.nlm.nih.gov/pubmed/17146610?dopt=Citation>

APA

Lochner, K., Gaemlich, A., Südel, K. M., Venzke, K., Moll, I., Knott, A., Stäb, F., Wenck, H., Döring, O., Böttger, M., & Gallinat, S. (2007). Expression of decorin and collagens I and III in different layers of human skin in vivo: a laser capture microdissection study. BIOGERONTOLOGY, 8(3), 269-282. [3]. http://www.ncbi.nlm.nih.gov/pubmed/17146610?dopt=Citation

Vancouver

Lochner K, Gaemlich A, Südel KM, Venzke K, Moll I, Knott A et al. Expression of decorin and collagens I and III in different layers of human skin in vivo: a laser capture microdissection study. BIOGERONTOLOGY. 2007;8(3):269-282. 3.

Bibtex

@article{681bab8fffb04a66a3bc5a83239bd77f,
title = "Expression of decorin and collagens I and III in different layers of human skin in vivo: a laser capture microdissection study.",
abstract = "Extracellular matrix (ECM) organization is a complex process that requires the coordinated efforts of many molecules. For the regulation of collagen fiber diameter, the proteoglycan decorin appears to be of major relevance. To investigate the role of decorin in the process of (photo-)aging in more detail, full-thickness punch biopsies were isolated from human buttock skin. Single exposure with two minimal erythemal doses of solar simulated irradiation caused down-regulation of decorin mRNA in young (n = 5) and old subjects (n = 5) after 24 h. Interestingly, decorin mRNA was elevated with age. To test the hypothesis that a decreased collagen-to-decorin-ratio impairs collagen structure we also investigated collagens I and III gene expression. Both were down-regulated with increasing age and after single UV-irradiation. As determined by laser capture microdissection-quantitative real time-Polymerase chain reaction (n = 11), decorin is mostly present in the reticular dermis while being absent from the papillary dermis. Minor expression was also observed in the epidermis. However, in contrast to full-thickness skin biopsies age-dependent changes in collagens I, III, and decorin expression could not be observed with this methodology indicating technical limitations. Together with our finding that collagens I and III mRNA are similarly expressed in the reticular and papillary dermis and are down-regulated by UV, our studies support the idea of a major role of decorin in ECM organization. Altered expression of decorin mRNA in the different dermal strata and a decrease in the collagen-to-decorin ratio inflicted by both age and ultraviolet irradiation possibly affect collagen bundle diameter and subsequently the mechanical properties of human skin.",
author = "Katrin Lochner and Astrid Gaemlich and S{\"u}del, {Kirstin Marie} and Kirsten Venzke and Ingrid Moll and Anja Knott and Franz St{\"a}b and Horst Wenck and Olaf D{\"o}ring and Michael B{\"o}ttger and Stefan Gallinat",
year = "2007",
language = "Deutsch",
volume = "8",
pages = "269--282",
number = "3",

}

RIS

TY - JOUR

T1 - Expression of decorin and collagens I and III in different layers of human skin in vivo: a laser capture microdissection study.

AU - Lochner, Katrin

AU - Gaemlich, Astrid

AU - Südel, Kirstin Marie

AU - Venzke, Kirsten

AU - Moll, Ingrid

AU - Knott, Anja

AU - Stäb, Franz

AU - Wenck, Horst

AU - Döring, Olaf

AU - Böttger, Michael

AU - Gallinat, Stefan

PY - 2007

Y1 - 2007

N2 - Extracellular matrix (ECM) organization is a complex process that requires the coordinated efforts of many molecules. For the regulation of collagen fiber diameter, the proteoglycan decorin appears to be of major relevance. To investigate the role of decorin in the process of (photo-)aging in more detail, full-thickness punch biopsies were isolated from human buttock skin. Single exposure with two minimal erythemal doses of solar simulated irradiation caused down-regulation of decorin mRNA in young (n = 5) and old subjects (n = 5) after 24 h. Interestingly, decorin mRNA was elevated with age. To test the hypothesis that a decreased collagen-to-decorin-ratio impairs collagen structure we also investigated collagens I and III gene expression. Both were down-regulated with increasing age and after single UV-irradiation. As determined by laser capture microdissection-quantitative real time-Polymerase chain reaction (n = 11), decorin is mostly present in the reticular dermis while being absent from the papillary dermis. Minor expression was also observed in the epidermis. However, in contrast to full-thickness skin biopsies age-dependent changes in collagens I, III, and decorin expression could not be observed with this methodology indicating technical limitations. Together with our finding that collagens I and III mRNA are similarly expressed in the reticular and papillary dermis and are down-regulated by UV, our studies support the idea of a major role of decorin in ECM organization. Altered expression of decorin mRNA in the different dermal strata and a decrease in the collagen-to-decorin ratio inflicted by both age and ultraviolet irradiation possibly affect collagen bundle diameter and subsequently the mechanical properties of human skin.

AB - Extracellular matrix (ECM) organization is a complex process that requires the coordinated efforts of many molecules. For the regulation of collagen fiber diameter, the proteoglycan decorin appears to be of major relevance. To investigate the role of decorin in the process of (photo-)aging in more detail, full-thickness punch biopsies were isolated from human buttock skin. Single exposure with two minimal erythemal doses of solar simulated irradiation caused down-regulation of decorin mRNA in young (n = 5) and old subjects (n = 5) after 24 h. Interestingly, decorin mRNA was elevated with age. To test the hypothesis that a decreased collagen-to-decorin-ratio impairs collagen structure we also investigated collagens I and III gene expression. Both were down-regulated with increasing age and after single UV-irradiation. As determined by laser capture microdissection-quantitative real time-Polymerase chain reaction (n = 11), decorin is mostly present in the reticular dermis while being absent from the papillary dermis. Minor expression was also observed in the epidermis. However, in contrast to full-thickness skin biopsies age-dependent changes in collagens I, III, and decorin expression could not be observed with this methodology indicating technical limitations. Together with our finding that collagens I and III mRNA are similarly expressed in the reticular and papillary dermis and are down-regulated by UV, our studies support the idea of a major role of decorin in ECM organization. Altered expression of decorin mRNA in the different dermal strata and a decrease in the collagen-to-decorin ratio inflicted by both age and ultraviolet irradiation possibly affect collagen bundle diameter and subsequently the mechanical properties of human skin.

M3 - SCORING: Zeitschriftenaufsatz

VL - 8

SP - 269

EP - 282

IS - 3

M1 - 3

ER -