Expression and localization of the multidrug resistance-associated protein 3 in rat small and large intestine
Standard
Expression and localization of the multidrug resistance-associated protein 3 in rat small and large intestine. / Rost, Daniel; Mahner, Sven; Sugiyama, Yuichi; Stremmel, Wolfgang.
In: AM J PHYSIOL-GASTR L, Vol. 282, No. 4, 01.04.2002, p. G720-6.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Expression and localization of the multidrug resistance-associated protein 3 in rat small and large intestine
AU - Rost, Daniel
AU - Mahner, Sven
AU - Sugiyama, Yuichi
AU - Stremmel, Wolfgang
PY - 2002/4/1
Y1 - 2002/4/1
N2 - Multidrug resistance-associated protein 3 (MRP3; symbol ABCC3), has been shown to mediate ATP-dependent transport of organic anions including 17beta-glucuronosyl estradiol, glucuronosyl bilirubin, monovalent, and sulfated bile salts. MRP3 mRNA expression was reported in rat intestine suggesting a role of MRP3 in the intestinal transport. We examined the expression and localization of MRP3 in rat small and large intestine by RT-PCR, immunofluorescence, and immunoblot analysis. MRP3 was identified in all intestinal segments by RT-PCR. MRP3 expression was low in duodenum and jejunum but markedly increased in ileum and colon. With the use of a rat MRP3 specific antibody, MRP3 was localized to the basolateral domains of enterocytes. Immunofluorescence analysis and immunoblot analysis confirmed a strong expression of rat MRP3 in ileum and colon. In contrast, MRP2 was predominantly expressed in the proximal segments of rat small intestine. Our findings demonstrate a high expression of rat MRP3 in ileum and colon and provide evidence for an involvement of MRP3 in the ATP-dependent transport of organic anions, including bile salts from the enterocyte into blood.
AB - Multidrug resistance-associated protein 3 (MRP3; symbol ABCC3), has been shown to mediate ATP-dependent transport of organic anions including 17beta-glucuronosyl estradiol, glucuronosyl bilirubin, monovalent, and sulfated bile salts. MRP3 mRNA expression was reported in rat intestine suggesting a role of MRP3 in the intestinal transport. We examined the expression and localization of MRP3 in rat small and large intestine by RT-PCR, immunofluorescence, and immunoblot analysis. MRP3 was identified in all intestinal segments by RT-PCR. MRP3 expression was low in duodenum and jejunum but markedly increased in ileum and colon. With the use of a rat MRP3 specific antibody, MRP3 was localized to the basolateral domains of enterocytes. Immunofluorescence analysis and immunoblot analysis confirmed a strong expression of rat MRP3 in ileum and colon. In contrast, MRP2 was predominantly expressed in the proximal segments of rat small intestine. Our findings demonstrate a high expression of rat MRP3 in ileum and colon and provide evidence for an involvement of MRP3 in the ATP-dependent transport of organic anions, including bile salts from the enterocyte into blood.
KW - Animals
KW - Colon
KW - Duodenum
KW - Fluorescent Antibody Technique
KW - Gene Expression
KW - Ileum
KW - Immunoblotting
KW - Intestinal Mucosa
KW - Intestines
KW - Jejunum
KW - Male
KW - Microscopy, Fluorescence
KW - Mitochondrial Proteins
KW - Multidrug Resistance-Associated Proteins
KW - Rats
KW - Rats, Wistar
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Ribosomal Proteins
KW - Saccharomyces cerevisiae Proteins
KW - Tissue Distribution
U2 - 10.1152/ajpgi.00318.2001
DO - 10.1152/ajpgi.00318.2001
M3 - SCORING: Journal article
C2 - 11897632
VL - 282
SP - G720-6
JO - AM J PHYSIOL-GASTR L
JF - AM J PHYSIOL-GASTR L
SN - 0193-1857
IS - 4
ER -
