Exp5 exports eEF1A via tRNA from nuclei and synergizes with other transport pathways to confine translation to the cytoplasm
Standard
Exp5 exports eEF1A via tRNA from nuclei and synergizes with other transport pathways to confine translation to the cytoplasm. / Bohnsack, Markus T; Regener, Kathrin; Schwappach, Blanche; Saffrich, Rainer; Paraskeva, Efrosyni; Hartmann, Enno; Görlich, Dirk.
In: EMBO J, Vol. 21, No. 22, 15.11.2002, p. 6205-15.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Exp5 exports eEF1A via tRNA from nuclei and synergizes with other transport pathways to confine translation to the cytoplasm
AU - Bohnsack, Markus T
AU - Regener, Kathrin
AU - Schwappach, Blanche
AU - Saffrich, Rainer
AU - Paraskeva, Efrosyni
AU - Hartmann, Enno
AU - Görlich, Dirk
PY - 2002/11/15
Y1 - 2002/11/15
N2 - Importin beta-type transport receptors mediate the vast majority of transport pathways between cell nucleus and cytoplasm. We identify here the translation elongation factor 1A (eEF1A) as the predominant nuclear export substrate of RanBP21/exportin 5 (Exp5). This cargo-exportin interaction is rather un usual in that eEF1A binds the exportin not directly, but instead via aminoacylated tRNAs. Exp5 thus represents the second directly RNA-binding exportin and mediates tRNA export in parallel with exportin-t. It was suggested recently that 10-15% of the cellular translation would occur in the nucleus. Our data rule out such a scenario and instead suggest that nuclear translation is actively suppressed by the nuclear export machinery. We found that the vast majority of translation initiation factors (eIF2, eIF2B, eIF3, eIF4A1, eIF5 and eIF5B), all three elongation factors (eEF1A, eEF1B and eEF2) and the termination factor eRF1 are strictly excluded from nuclei. Besides Exp5 and importin 13, CRM1 and as yet unidentified exportins also contribute to the depletion of translation factors from nuclei.
AB - Importin beta-type transport receptors mediate the vast majority of transport pathways between cell nucleus and cytoplasm. We identify here the translation elongation factor 1A (eEF1A) as the predominant nuclear export substrate of RanBP21/exportin 5 (Exp5). This cargo-exportin interaction is rather un usual in that eEF1A binds the exportin not directly, but instead via aminoacylated tRNAs. Exp5 thus represents the second directly RNA-binding exportin and mediates tRNA export in parallel with exportin-t. It was suggested recently that 10-15% of the cellular translation would occur in the nucleus. Our data rule out such a scenario and instead suggest that nuclear translation is actively suppressed by the nuclear export machinery. We found that the vast majority of translation initiation factors (eIF2, eIF2B, eIF3, eIF4A1, eIF5 and eIF5B), all three elongation factors (eEF1A, eEF1B and eEF2) and the termination factor eRF1 are strictly excluded from nuclei. Besides Exp5 and importin 13, CRM1 and as yet unidentified exportins also contribute to the depletion of translation factors from nuclei.
KW - 3T3 Cells
KW - Active Transport, Cell Nucleus/physiology
KW - Animals
KW - Cell Nucleus/metabolism
KW - Cells, Cultured
KW - Cloning, Molecular
KW - Cricetinae
KW - Cytoplasm/metabolism
KW - Drosophila Proteins/genetics
KW - Drosophila melanogaster/cytology
KW - Eukaryotic Initiation Factors/metabolism
KW - Expressed Sequence Tags
KW - Guanosine Triphosphate/metabolism
KW - HeLa Cells
KW - Humans
KW - Karyopherins/genetics
KW - Macromolecular Substances
KW - Mesocricetus
KW - Mice
KW - Molecular Sequence Data
KW - Peptide Elongation Factor 1/metabolism
KW - Protein Biosynthesis
KW - Protein Interaction Mapping
KW - Protein Isoforms/metabolism
KW - RNA, Transfer, Amino Acyl/metabolism
KW - Receptors, Cytoplasmic and Nuclear
KW - Recombinant Fusion Proteins/physiology
KW - ran GTP-Binding Protein/metabolism
U2 - 10.1093/emboj/cdf613
DO - 10.1093/emboj/cdf613
M3 - SCORING: Journal article
C2 - 12426392
VL - 21
SP - 6205
EP - 6215
JO - EMBO J
JF - EMBO J
SN - 0261-4189
IS - 22
ER -