Evaluation of different diagnostic tools for the detection and identification of Riemerella anatipestifer
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Evaluation of different diagnostic tools for the detection and identification of Riemerella anatipestifer. / Rubbenstroth, Dennis; Ryll, Martin; Knobloch, Johannes K.-M.; Köhler, Bernd; Rautenschlein, Silke.
In: AVIAN PATHOL, Vol. 42, No. 1, 02.2013, p. 17-26.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Evaluation of different diagnostic tools for the detection and identification of Riemerella anatipestifer
AU - Rubbenstroth, Dennis
AU - Ryll, Martin
AU - Knobloch, Johannes K.-M.
AU - Köhler, Bernd
AU - Rautenschlein, Silke
PY - 2013/2
Y1 - 2013/2
N2 - Riemerella anatipestifer (RA) is an important avian pathogen with considerable impact on poultry production worldwide. However, the diagnosis of RA infections may be difficult, mainly due to problems with unequivocal differentiation of RA from other Flavobacteriaceae and a lack of standardized methods and reagents. The aim of the present study was therefore to complement the routine diagnostic strategies for RA by design and evaluation of alternative diagnostic tools. We designed and validated a new RA-specific polymerase chain reaction assay, which proved to be a valuable tool for the identification of RA isolates as well as for rapid and sensitive RA detection directly from diagnostic samples. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry fingerprinting of whole bacterial cells was also demonstrated to identify RA isolates efficiently. Furthermore, this method may also provide opportunities for RA subtyping. In our study, a stable subcluster was formed by the mass spectroscopy profiles of a group of RA isolates originating from turkey flocks in northern Germany, suggesting an epidemiological relationship of these isolates. Serotyping is a further important measure to characterize RA isolates. We tested a set of commercially available anti-RA sera with RA serotype reference strains and field isolates to allow comparison between these sera and reference sera. In summary, this report contributes to the improvement of present microbiological and molecular strategies for the diagnosis of RA infections by providing new tools as well as enhanced knowledge on existing methods.
AB - Riemerella anatipestifer (RA) is an important avian pathogen with considerable impact on poultry production worldwide. However, the diagnosis of RA infections may be difficult, mainly due to problems with unequivocal differentiation of RA from other Flavobacteriaceae and a lack of standardized methods and reagents. The aim of the present study was therefore to complement the routine diagnostic strategies for RA by design and evaluation of alternative diagnostic tools. We designed and validated a new RA-specific polymerase chain reaction assay, which proved to be a valuable tool for the identification of RA isolates as well as for rapid and sensitive RA detection directly from diagnostic samples. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry fingerprinting of whole bacterial cells was also demonstrated to identify RA isolates efficiently. Furthermore, this method may also provide opportunities for RA subtyping. In our study, a stable subcluster was formed by the mass spectroscopy profiles of a group of RA isolates originating from turkey flocks in northern Germany, suggesting an epidemiological relationship of these isolates. Serotyping is a further important measure to characterize RA isolates. We tested a set of commercially available anti-RA sera with RA serotype reference strains and field isolates to allow comparison between these sera and reference sera. In summary, this report contributes to the improvement of present microbiological and molecular strategies for the diagnosis of RA infections by providing new tools as well as enhanced knowledge on existing methods.
KW - Animals
KW - Bacterial Typing Techniques
KW - Base Sequence
KW - Flavobacteriaceae Infections
KW - Germany
KW - Immune Sera
KW - Molecular Sequence Data
KW - Polymerase Chain Reaction
KW - Poultry Diseases
KW - Riemerella
KW - Sensitivity and Specificity
KW - Sequence Analysis, DNA
KW - Serotyping
KW - Species Specificity
KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
KW - Time Factors
KW - Turkeys
KW - Evaluation Studies
KW - Journal Article
U2 - 10.1080/03079457.2012.752066
DO - 10.1080/03079457.2012.752066
M3 - SCORING: Journal article
C2 - 23391177
VL - 42
SP - 17
EP - 26
JO - AVIAN PATHOL
JF - AVIAN PATHOL
SN - 0307-9457
IS - 1
ER -
