Establishment and Characterization of a Pair of Patient-derived Human Non-small Cell Lung Cancer Cell Lines from a Primary Tumor and Corresponding Lymph Node Metastasis

Florian Gebauer; Daniel Wicklein; Michael Tachezy; Tobias Grob; Doris Steinemann; Georgi Manukjan; Gudrun Göhring; Brigitte Schlegelberger; Hanna Maar; Jakob R Izbicki; Maximilian Bockhorn; Udo Schumacher

Establishment and Characterization of a Pair of Patient-derived Human Non-small Cell Lung Cancer Cell Lines from a Primary Tumor and Corresponding Lymph Node Metastasis

Standard

Establishment and Characterization of a Pair of Patient-derived Human Non-small Cell Lung Cancer Cell Lines from a Primary Tumor and Corresponding Lymph Node Metastasis. / Gebauer, Florian; Wicklein, Daniel; Tachezy, Michael; Grob, Tobias; Steinemann, Doris; Manukjan, Georgi; Göhring, Gudrun; Schlegelberger, Brigitte; Maar, Hanna; Izbicki, Jakob R; Bockhorn, Maximilian; Schumacher, Udo.

In: ANTICANCER RES, Vol. 36, No. 4, 04.2016, p. 1507-18.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Gebauer, F, Wicklein, D, Tachezy, M, Grob, T, Steinemann, D, Manukjan, G, Göhring, G, Schlegelberger, B, Maar, H, Izbicki, JR, Bockhorn, M & Schumacher, U 2016, 'Establishment and Characterization of a Pair of Patient-derived Human Non-small Cell Lung Cancer Cell Lines from a Primary Tumor and Corresponding Lymph Node Metastasis', ANTICANCER RES, vol. 36, no. 4, pp. 1507-18.

APA

Gebauer, F., Wicklein, D., Tachezy, M., Grob, T., Steinemann, D., Manukjan, G., Göhring, G., Schlegelberger, B., Maar, H., Izbicki, J. R., Bockhorn, M., & Schumacher, U. (2016). Establishment and Characterization of a Pair of Patient-derived Human Non-small Cell Lung Cancer Cell Lines from a Primary Tumor and Corresponding Lymph Node Metastasis. ANTICANCER RES, 36(4), 1507-18.

Vancouver

Gebauer F, Wicklein D, Tachezy M, Grob T, Steinemann D, Manukjan G et al. Establishment and Characterization of a Pair of Patient-derived Human Non-small Cell Lung Cancer Cell Lines from a Primary Tumor and Corresponding Lymph Node Metastasis. ANTICANCER RES. 2016 Apr;36(4):1507-18.

Bibtex

@article{be5b787994554e7fbaec858a43a00bde,

title = "Establishment and Characterization of a Pair of Patient-derived Human Non-small Cell Lung Cancer Cell Lines from a Primary Tumor and Corresponding Lymph Node Metastasis",

abstract = "BACKGROUND: Non-small lung cancer is the leading cause of cancer-related mortality worldwide. For a deeper understanding of tumor biology, we established a pair of cell lines derived from a primary tumor and a corresponding lymph node metastasis.MATERIAL AND METHODS: The cell line BC4323 from the primary tumor (PT) and a mediastinal lymph node metastasis (LN) were derived from an adenocarcinoma (pT2, pN2, G3, UICC stage IIIa) in a 47-year-old female patient. Comparative characterization was performed by in vitro analysis. A murine xenograft was established for analysis of in vivo behavior.RESULTS: Chromosomal aberrations were detected in multiple chromosomal sections throughout the entire genome, with only a few differences between PT and LN cells. High-level Kirsten ras oncogene homolog (KRAS) mutation and amplification were seen based on a chromosomal translocation and novel assembled chromosome. In contrast to the genomic level, at the mRNA and protein levels, multiple differences were detectable, in particular in markers for cell adhesion [e.g. epithelial cell adhesion molecule (EpCAM), CD44, P-selectin binding, epidermal growth factor receptor (EGFR) and integrin alphaV] and the epithelial-mesenchymal transition. Due to accelerated tumor growth in vivo by the PT cells, a shortened overall survival was seen (60 vs. 101 days, p=0.005).CONCLUSION: We provide detailed analysis of a cell line derived from a primary tumor and a corresponding LN metastasis. This unique feature allows further investigative analysis of the differences and regulatory processes underlying the metastatic process during tumor progression in non-small cell lung cancer.",

author = "Florian Gebauer and Daniel Wicklein and Michael Tachezy and Tobias Grob and Doris Steinemann and Georgi Manukjan and Gudrun G{\"o}hring and Brigitte Schlegelberger and Hanna Maar and Izbicki, {Jakob R} and Maximilian Bockhorn and Udo Schumacher",

year = "2016",

month = apr,

language = "English",

volume = "36",

pages = "1507--18",

journal = "ANTICANCER RES",

issn = "0250-7005",

publisher = "International Institute of Anticancer Research",

number = "4",

}

RIS

TY - JOUR

T1 - Establishment and Characterization of a Pair of Patient-derived Human Non-small Cell Lung Cancer Cell Lines from a Primary Tumor and Corresponding Lymph Node Metastasis

AU - Gebauer, Florian

AU - Wicklein, Daniel

AU - Tachezy, Michael

AU - Grob, Tobias

AU - Steinemann, Doris

AU - Manukjan, Georgi

AU - Göhring, Gudrun

AU - Schlegelberger, Brigitte

AU - Maar, Hanna

AU - Izbicki, Jakob R

AU - Bockhorn, Maximilian

AU - Schumacher, Udo

PY - 2016/4

Y1 - 2016/4

N2 - BACKGROUND: Non-small lung cancer is the leading cause of cancer-related mortality worldwide. For a deeper understanding of tumor biology, we established a pair of cell lines derived from a primary tumor and a corresponding lymph node metastasis.MATERIAL AND METHODS: The cell line BC4323 from the primary tumor (PT) and a mediastinal lymph node metastasis (LN) were derived from an adenocarcinoma (pT2, pN2, G3, UICC stage IIIa) in a 47-year-old female patient. Comparative characterization was performed by in vitro analysis. A murine xenograft was established for analysis of in vivo behavior.RESULTS: Chromosomal aberrations were detected in multiple chromosomal sections throughout the entire genome, with only a few differences between PT and LN cells. High-level Kirsten ras oncogene homolog (KRAS) mutation and amplification were seen based on a chromosomal translocation and novel assembled chromosome. In contrast to the genomic level, at the mRNA and protein levels, multiple differences were detectable, in particular in markers for cell adhesion [e.g. epithelial cell adhesion molecule (EpCAM), CD44, P-selectin binding, epidermal growth factor receptor (EGFR) and integrin alphaV] and the epithelial-mesenchymal transition. Due to accelerated tumor growth in vivo by the PT cells, a shortened overall survival was seen (60 vs. 101 days, p=0.005).CONCLUSION: We provide detailed analysis of a cell line derived from a primary tumor and a corresponding LN metastasis. This unique feature allows further investigative analysis of the differences and regulatory processes underlying the metastatic process during tumor progression in non-small cell lung cancer.

AB - BACKGROUND: Non-small lung cancer is the leading cause of cancer-related mortality worldwide. For a deeper understanding of tumor biology, we established a pair of cell lines derived from a primary tumor and a corresponding lymph node metastasis.MATERIAL AND METHODS: The cell line BC4323 from the primary tumor (PT) and a mediastinal lymph node metastasis (LN) were derived from an adenocarcinoma (pT2, pN2, G3, UICC stage IIIa) in a 47-year-old female patient. Comparative characterization was performed by in vitro analysis. A murine xenograft was established for analysis of in vivo behavior.RESULTS: Chromosomal aberrations were detected in multiple chromosomal sections throughout the entire genome, with only a few differences between PT and LN cells. High-level Kirsten ras oncogene homolog (KRAS) mutation and amplification were seen based on a chromosomal translocation and novel assembled chromosome. In contrast to the genomic level, at the mRNA and protein levels, multiple differences were detectable, in particular in markers for cell adhesion [e.g. epithelial cell adhesion molecule (EpCAM), CD44, P-selectin binding, epidermal growth factor receptor (EGFR) and integrin alphaV] and the epithelial-mesenchymal transition. Due to accelerated tumor growth in vivo by the PT cells, a shortened overall survival was seen (60 vs. 101 days, p=0.005).CONCLUSION: We provide detailed analysis of a cell line derived from a primary tumor and a corresponding LN metastasis. This unique feature allows further investigative analysis of the differences and regulatory processes underlying the metastatic process during tumor progression in non-small cell lung cancer.

M3 - SCORING: Journal article

C2 - 27069126

VL - 36

SP - 1507

EP - 1518

JO - ANTICANCER RES

JF - ANTICANCER RES

SN - 0250-7005

IS - 4

ER -