Epidermis-Derived Semaphorin Promotes Dendrite Self-Avoidance by Regulating Dendrite-Substrate Adhesion in Drosophila Sensory Neurons
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Epidermis-Derived Semaphorin Promotes Dendrite Self-Avoidance by Regulating Dendrite-Substrate Adhesion in Drosophila Sensory Neurons. / Meltzer, Shan; Yadav, Smita; Lee, Jiae; Soba, Peter; Younger, Susan H; Jin, Peng; Zhang, Wei; Parrish, Jay; Jan, Lily Yeh; Jan, Yuh-Nung.
In: NEURON, Vol. 89, No. 4, 17.02.2016, p. 741-55.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Epidermis-Derived Semaphorin Promotes Dendrite Self-Avoidance by Regulating Dendrite-Substrate Adhesion in Drosophila Sensory Neurons
AU - Meltzer, Shan
AU - Yadav, Smita
AU - Lee, Jiae
AU - Soba, Peter
AU - Younger, Susan H
AU - Jin, Peng
AU - Zhang, Wei
AU - Parrish, Jay
AU - Jan, Lily Yeh
AU - Jan, Yuh-Nung
N1 - Copyright © 2016 Elsevier Inc. All rights reserved.
PY - 2016/2/17
Y1 - 2016/2/17
N2 - Precise patterning of dendritic arbors is critical for the wiring and function of neural circuits. Dendrite-extracellular matrix (ECM) adhesion ensures that the dendrites of Drosophila dendritic arborization (da) sensory neurons are properly restricted in a 2D space, and thereby facilitates contact-mediated dendritic self-avoidance and tiling. However, the mechanisms regulating dendrite-ECM adhesion in vivo are poorly understood. Here, we show that mutations in the semaphorin ligand sema-2b lead to a dramatic increase in self-crossing of dendrites due to defects in dendrite-ECM adhesion, resulting in a failure to confine dendrites to a 2D plane. Furthermore, we find that Sema-2b is secreted from the epidermis and signals through the Plexin B receptor in neighboring neurons. Importantly, we find that Sema-2b/PlexB genetically and physically interacts with TORC2 complex, Tricornered (Trc) kinase, and integrins. These results reveal a novel role for semaphorins in dendrite patterning and illustrate how epidermal-derived cues regulate neural circuit assembly.
AB - Precise patterning of dendritic arbors is critical for the wiring and function of neural circuits. Dendrite-extracellular matrix (ECM) adhesion ensures that the dendrites of Drosophila dendritic arborization (da) sensory neurons are properly restricted in a 2D space, and thereby facilitates contact-mediated dendritic self-avoidance and tiling. However, the mechanisms regulating dendrite-ECM adhesion in vivo are poorly understood. Here, we show that mutations in the semaphorin ligand sema-2b lead to a dramatic increase in self-crossing of dendrites due to defects in dendrite-ECM adhesion, resulting in a failure to confine dendrites to a 2D plane. Furthermore, we find that Sema-2b is secreted from the epidermis and signals through the Plexin B receptor in neighboring neurons. Importantly, we find that Sema-2b/PlexB genetically and physically interacts with TORC2 complex, Tricornered (Trc) kinase, and integrins. These results reveal a novel role for semaphorins in dendrite patterning and illustrate how epidermal-derived cues regulate neural circuit assembly.
KW - Animals
KW - Animals, Genetically Modified
KW - Cell Communication
KW - Dendrites
KW - Drosophila
KW - Drosophila Proteins
KW - Epidermis
KW - Focal Adhesion Kinase 1
KW - Gene Expression Regulation, Developmental
KW - Green Fluorescent Proteins
KW - Immunoprecipitation
KW - Larva
KW - Molecular Biology
KW - Multiprotein Complexes
KW - Mutation
KW - Nerve Tissue Proteins
KW - Receptors, Cell Surface
KW - Semaphorins
KW - Sensory Receptor Cells
KW - TOR Serine-Threonine Kinases
KW - Transfection
KW - Journal Article
KW - Research Support, N.I.H., Extramural
KW - Research Support, Non-U.S. Gov't
KW - Research Support, U.S. Gov't, Non-P.H.S.
U2 - 10.1016/j.neuron.2016.01.020
DO - 10.1016/j.neuron.2016.01.020
M3 - SCORING: Journal article
C2 - 26853303
VL - 89
SP - 741
EP - 755
JO - NEURON
JF - NEURON
SN - 0896-6273
IS - 4
ER -