Research ExplorerPublicationsEpidermal growth factor receptor overexpression is common an...

Epidermal growth factor receptor overexpression is common and not correlated to gene copy number in ependymoma

Standard

Epidermal growth factor receptor overexpression is common and not correlated to gene copy number in ependymoma. / Friedrich, Carsten; von Bueren, André O; Kolevatova, Larissa; Bernreuther, Christian; Grob, Tobias; Sepulveda-Falla, Diego; van den Boom, Leander; Westphal, Manfred; Simon, Ronald; Glatzel, Markus.

In: CHILD NERV SYST, Vol. 32, No. 2, 02.2016, p. 281-90.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Friedrich, C, von Bueren, AO, Kolevatova, L, Bernreuther, C, Grob, T, Sepulveda-Falla, D, van den Boom, L, Westphal, M, Simon, R & Glatzel, M 2016, 'Epidermal growth factor receptor overexpression is common and not correlated to gene copy number in ependymoma', CHILD NERV SYST, vol. 32, no. 2, pp. 281-90. https://doi.org/10.1007/s00381-015-2981-2

APA

Friedrich, C., von Bueren, A. O., Kolevatova, L., Bernreuther, C., Grob, T., Sepulveda-Falla, D., van den Boom, L., Westphal, M., Simon, R., & Glatzel, M. (2016). Epidermal growth factor receptor overexpression is common and not correlated to gene copy number in ependymoma. CHILD NERV SYST, 32(2), 281-90. https://doi.org/10.1007/s00381-015-2981-2

Vancouver

Friedrich C, von Bueren AO, Kolevatova L, Bernreuther C, Grob T, Sepulveda-Falla D et al. Epidermal growth factor receptor overexpression is common and not correlated to gene copy number in ependymoma. CHILD NERV SYST. 2016 Feb;32(2):281-90. https://doi.org/10.1007/s00381-015-2981-2

Bibtex

@article{28add79b45dc49f9be7366f0f50ab3fb,
title = "Epidermal growth factor receptor overexpression is common and not correlated to gene copy number in ependymoma",
abstract = "PURPOSE: The aim of this study was to investigate the epidermal growth factor receptor (EGFR) status in ependymoma specimens, as there is a need for new prognostic and druggable targets in this disease.METHODS: Ependymomas (WHO grade II, n = 40; WHO grade III, n = 15) located spinal (n = 35), infratentorial (n = 14), and supratentorial (n = 6) of 53 patients with a median age of 40 (range, 2-79) years were analyzed for Ki-67, p53, and EGFR expression by immunohistochemistry using a tissue microarray and for EGFR gene copy number alterations/mutations. Results were correlated to clinical data.RESULTS: EGFR overexpression was found in 30/60 % of ependymomas depending on the antibody used and was more pronounced in WHO grade III. High EGFR gene copy number gains were found in 6 (11 %) ependymomas with half of them being amplifications. EGFR amplified ependymomas displayed an EGFR overexpression with both antibodies in two of three cases. A missense mutation in exon 20 of EGFR (S768I) was detected in one amplified case.CONCLUSIONS: EGFR is frequently overexpressed in ependymomas. Other mechanisms than amplification of the EGFR gene appear to contribute to EGFR overexpression in most cases. EGFR mutations may be present in a small subset of ependymomas.",
author = "Carsten Friedrich and {von Bueren}, {Andr{\'e} O} and Larissa Kolevatova and Christian Bernreuther and Tobias Grob and Diego Sepulveda-Falla and {van den Boom}, Leander and Manfred Westphal and Ronald Simon and Markus Glatzel",
year = "2016",
month = feb,
doi = "10.1007/s00381-015-2981-2",
language = "English",
volume = "32",
pages = "281--90",
journal = "CHILD NERV SYST",
issn = "0256-7040",
publisher = "Springer",
number = "2",

}

RIS

TY - JOUR

T1 - Epidermal growth factor receptor overexpression is common and not correlated to gene copy number in ependymoma

AU - Friedrich, Carsten

AU - von Bueren, André O

AU - Kolevatova, Larissa

AU - Bernreuther, Christian

AU - Grob, Tobias

AU - Sepulveda-Falla, Diego

AU - van den Boom, Leander

AU - Westphal, Manfred

AU - Simon, Ronald

AU - Glatzel, Markus

PY - 2016/2

Y1 - 2016/2

N2 - PURPOSE: The aim of this study was to investigate the epidermal growth factor receptor (EGFR) status in ependymoma specimens, as there is a need for new prognostic and druggable targets in this disease.METHODS: Ependymomas (WHO grade II, n = 40; WHO grade III, n = 15) located spinal (n = 35), infratentorial (n = 14), and supratentorial (n = 6) of 53 patients with a median age of 40 (range, 2-79) years were analyzed for Ki-67, p53, and EGFR expression by immunohistochemistry using a tissue microarray and for EGFR gene copy number alterations/mutations. Results were correlated to clinical data.RESULTS: EGFR overexpression was found in 30/60 % of ependymomas depending on the antibody used and was more pronounced in WHO grade III. High EGFR gene copy number gains were found in 6 (11 %) ependymomas with half of them being amplifications. EGFR amplified ependymomas displayed an EGFR overexpression with both antibodies in two of three cases. A missense mutation in exon 20 of EGFR (S768I) was detected in one amplified case.CONCLUSIONS: EGFR is frequently overexpressed in ependymomas. Other mechanisms than amplification of the EGFR gene appear to contribute to EGFR overexpression in most cases. EGFR mutations may be present in a small subset of ependymomas.

AB - PURPOSE: The aim of this study was to investigate the epidermal growth factor receptor (EGFR) status in ependymoma specimens, as there is a need for new prognostic and druggable targets in this disease.METHODS: Ependymomas (WHO grade II, n = 40; WHO grade III, n = 15) located spinal (n = 35), infratentorial (n = 14), and supratentorial (n = 6) of 53 patients with a median age of 40 (range, 2-79) years were analyzed for Ki-67, p53, and EGFR expression by immunohistochemistry using a tissue microarray and for EGFR gene copy number alterations/mutations. Results were correlated to clinical data.RESULTS: EGFR overexpression was found in 30/60 % of ependymomas depending on the antibody used and was more pronounced in WHO grade III. High EGFR gene copy number gains were found in 6 (11 %) ependymomas with half of them being amplifications. EGFR amplified ependymomas displayed an EGFR overexpression with both antibodies in two of three cases. A missense mutation in exon 20 of EGFR (S768I) was detected in one amplified case.CONCLUSIONS: EGFR is frequently overexpressed in ependymomas. Other mechanisms than amplification of the EGFR gene appear to contribute to EGFR overexpression in most cases. EGFR mutations may be present in a small subset of ependymomas.

U2 - 10.1007/s00381-015-2981-2

DO - 10.1007/s00381-015-2981-2

M3 - SCORING: Journal article

C2 - 26686534

VL - 32

SP - 281

EP - 290

JO - CHILD NERV SYST

JF - CHILD NERV SYST

SN - 0256-7040

IS - 2

ER -