Energy deprivation transiently enhances rhythmic inhibitory events in the CA3 hippocampal network in vitro
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Energy deprivation transiently enhances rhythmic inhibitory events in the CA3 hippocampal network in vitro. / Gee, C E; Benquet, P; Demont-Guignard, S; Wendling, F; Gerber, U.
In: NEUROSCIENCE, Vol. 168, No. 3, 14.07.2010, p. 605-12.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Energy deprivation transiently enhances rhythmic inhibitory events in the CA3 hippocampal network in vitro
AU - Gee, C E
AU - Benquet, P
AU - Demont-Guignard, S
AU - Wendling, F
AU - Gerber, U
N1 - Copyright 2010 IBRO. Published by Elsevier Ltd. All rights reserved.
PY - 2010/7/14
Y1 - 2010/7/14
N2 - Oxygen glucose deprivation (OGD) leads to rapid suppression of synaptic transmission. Here we describe an emergence of rhythmic activity at 8 to 20 Hz in the CA3 subfield of hippocampal slice cultures occurring for a few minutes prior to the OGD-induced cessation of evoked responses. These oscillations, dominated by inhibitory events, represent network activity, as they were abolished by tetrodotoxin. They were also completely blocked by the GABAergic antagonist picrotoxin, and strongly reduced by the glutamatergic antagonist NBQX. Applying CPP to block NMDA receptors had no effect and neither did UBP302, an antagonist of GluK1-containing kainate receptors. The gap junction blocker mefloquine disrupted rhythmicity. Simultaneous whole-cell voltage-clamp recordings from neighboring or distant CA3 pyramidal cells revealed strong cross-correlation of the incoming rhythmic activity. Interneurons in the CA3 area received similar correlated activity. Interestingly, oscillations were much less frequently observed in the CA1 area. These data, together with the observation that the recorded activity consists primarily of inhibitory events, suggest that CA3 interneurons are important for generating these oscillations. This transient increase in inhibitory network activity during OGD may represent a mechanism contributing to the lower vulnerability to ischemic insults of the CA3 area as compared to the CA1 area.
AB - Oxygen glucose deprivation (OGD) leads to rapid suppression of synaptic transmission. Here we describe an emergence of rhythmic activity at 8 to 20 Hz in the CA3 subfield of hippocampal slice cultures occurring for a few minutes prior to the OGD-induced cessation of evoked responses. These oscillations, dominated by inhibitory events, represent network activity, as they were abolished by tetrodotoxin. They were also completely blocked by the GABAergic antagonist picrotoxin, and strongly reduced by the glutamatergic antagonist NBQX. Applying CPP to block NMDA receptors had no effect and neither did UBP302, an antagonist of GluK1-containing kainate receptors. The gap junction blocker mefloquine disrupted rhythmicity. Simultaneous whole-cell voltage-clamp recordings from neighboring or distant CA3 pyramidal cells revealed strong cross-correlation of the incoming rhythmic activity. Interneurons in the CA3 area received similar correlated activity. Interestingly, oscillations were much less frequently observed in the CA1 area. These data, together with the observation that the recorded activity consists primarily of inhibitory events, suggest that CA3 interneurons are important for generating these oscillations. This transient increase in inhibitory network activity during OGD may represent a mechanism contributing to the lower vulnerability to ischemic insults of the CA3 area as compared to the CA1 area.
KW - Animals
KW - CA3 Region, Hippocampal
KW - Gap Junctions
KW - Glucose
KW - In Vitro Techniques
KW - Interneurons
KW - Oxygen
KW - Patch-Clamp Techniques
KW - Periodicity
KW - Pyramidal Cells
KW - Rats
KW - Rats, Wistar
U2 - 10.1016/j.neuroscience.2010.04.021
DO - 10.1016/j.neuroscience.2010.04.021
M3 - SCORING: Journal article
C2 - 20403414
VL - 168
SP - 605
EP - 612
JO - NEUROSCIENCE
JF - NEUROSCIENCE
SN - 0306-4522
IS - 3
ER -