ENDOXY - Development of a Biomimetic Oxygenator-Test-Device
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ENDOXY - Development of a Biomimetic Oxygenator-Test-Device. / Dietrich, Maren; Finocchiaro, Nicole; Olszweski, Sebastian; Arens, Jutta; Schmitz-Rode, Thomas; Sachweh, Joerg; Jockenhoevel, Stefan; Cornelissen, Christian G.
In: PLOS ONE, Vol. 10, No. 12, 2015, p. e0142961.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - ENDOXY - Development of a Biomimetic Oxygenator-Test-Device
AU - Dietrich, Maren
AU - Finocchiaro, Nicole
AU - Olszweski, Sebastian
AU - Arens, Jutta
AU - Schmitz-Rode, Thomas
AU - Sachweh, Joerg
AU - Jockenhoevel, Stefan
AU - Cornelissen, Christian G
PY - 2015
Y1 - 2015
N2 - OBJECTIVE: This study focusses on the development of a biomimetic oxygenator test device. Due to limited biocompatibility, current oxygenators do not allow mid- to long-term therapy. Tissue engineering uses autologous cell sources to overcome the immunogenic barriers of biomaterials. Surface coating with endothelial cells might improve hemocompatibility and thus prevent immunogenic reactions of the body. In this study this concept is applied to endothelialise a gas-permeable membrane to develop a biomimetic oxygenator test-device (ENDOXY).METHODS: ENDOXY-a multifunctional test-system was developed to endothelialise a gas-permeable membrane suitable for cell culture and to test the cell retention under shear stress and to measure gas transfer through it.RESULTS: Successful endothelialisation of the membrane was achieved and cells showed characteristic endothelial morphologies. They stained positive for endothelial markers. The number of cells aligned with shear stress and cell retention after blood perfusing experiments was high. Gas transfer is observed via uncoated and endothelialised membranes.CONCLUSION: The study showed promising results with regard to system design, endothelialisation, and cell retention under shear stress conditions. It strongly encourages further research into the system by testing different membrane materials to design a biomimetic membrane surface and pave way for a fully hemocompatible oxygenator.
AB - OBJECTIVE: This study focusses on the development of a biomimetic oxygenator test device. Due to limited biocompatibility, current oxygenators do not allow mid- to long-term therapy. Tissue engineering uses autologous cell sources to overcome the immunogenic barriers of biomaterials. Surface coating with endothelial cells might improve hemocompatibility and thus prevent immunogenic reactions of the body. In this study this concept is applied to endothelialise a gas-permeable membrane to develop a biomimetic oxygenator test-device (ENDOXY).METHODS: ENDOXY-a multifunctional test-system was developed to endothelialise a gas-permeable membrane suitable for cell culture and to test the cell retention under shear stress and to measure gas transfer through it.RESULTS: Successful endothelialisation of the membrane was achieved and cells showed characteristic endothelial morphologies. They stained positive for endothelial markers. The number of cells aligned with shear stress and cell retention after blood perfusing experiments was high. Gas transfer is observed via uncoated and endothelialised membranes.CONCLUSION: The study showed promising results with regard to system design, endothelialisation, and cell retention under shear stress conditions. It strongly encourages further research into the system by testing different membrane materials to design a biomimetic membrane surface and pave way for a fully hemocompatible oxygenator.
KW - Biomimetic Materials
KW - Endothelial Cells/cytology
KW - Equipment Design
KW - Materials Testing/instrumentation
KW - Oxygenators, Membrane
KW - Stress, Mechanical
KW - Tissue Engineering/instrumentation
U2 - 10.1371/journal.pone.0142961
DO - 10.1371/journal.pone.0142961
M3 - SCORING: Journal article
C2 - 26682907
VL - 10
SP - e0142961
JO - PLOS ONE
JF - PLOS ONE
SN - 1932-6203
IS - 12
ER -
