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Endoplasmic reticulum visits highly active spines and prevents runaway potentiation of synapses

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Endoplasmic reticulum visits highly active spines and prevents runaway potentiation of synapses. / Perez-Alvarez, Alberto; Yin, Shuting; Schulze, Christian; Hammer, John A; Wagner, Wolfgang; Oertner, Thomas G.

In: NAT COMMUN, Vol. 11, No. 1, 08.10.2020, p. 5083.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Perez-Alvarez, A, Yin, S, Schulze, C, Hammer, JA, Wagner, W & Oertner, TG 2020, 'Endoplasmic reticulum visits highly active spines and prevents runaway potentiation of synapses', NAT COMMUN, vol. 11, no. 1, pp. 5083. https://doi.org/10.1038/s41467-020-18889-5

APA

Perez-Alvarez, A., Yin, S., Schulze, C., Hammer, J. A., Wagner, W., & Oertner, T. G. (2020). Endoplasmic reticulum visits highly active spines and prevents runaway potentiation of synapses. NAT COMMUN, 11(1), 5083. https://doi.org/10.1038/s41467-020-18889-5

Vancouver

Perez-Alvarez A, Yin S, Schulze C, Hammer JA, Wagner W, Oertner TG. Endoplasmic reticulum visits highly active spines and prevents runaway potentiation of synapses. NAT COMMUN. 2020 Oct 8;11(1):5083. https://doi.org/10.1038/s41467-020-18889-5

Bibtex

@article{e0d7425031494ff7bee873adb853c69b,
title = "Endoplasmic reticulum visits highly active spines and prevents runaway potentiation of synapses",
abstract = "In hippocampal pyramidal cells, a small subset of dendritic spines contain endoplasmic reticulum (ER). In large spines, ER frequently forms a spine apparatus, while smaller spines contain just a single tubule of smooth ER. Here we show that the ER visits dendritic spines in a non-random manner, targeting spines during periods of high synaptic activity. When we blocked ER motility using a dominant negative approach against myosin V, spine synapses became stronger compared to controls. We were not able to further potentiate these maxed-out synapses, but long-term depression (LTD) was readily induced by low-frequency stimulation. We conclude that the brief ER visits to active spines have the important function of preventing runaway potentiation of individual spine synapses, keeping most of them at an intermediate strength level from which both long-term potentiation (LTP) and LTD are possible.",
keywords = "Animals, Dendritic Spines/metabolism, Endoplasmic Reticulum/metabolism, Hippocampus/metabolism, Long-Term Potentiation, Myosin Type V/metabolism, Rats, Wistar, Synapses/metabolism, Time-Lapse Imaging",
author = "Alberto Perez-Alvarez and Shuting Yin and Christian Schulze and Hammer, {John A} and Wolfgang Wagner and Oertner, {Thomas G}",
year = "2020",
month = oct,
day = "8",
doi = "10.1038/s41467-020-18889-5",
language = "English",
volume = "11",
pages = "5083",
journal = "NAT COMMUN",
issn = "2041-1723",
publisher = "NATURE PUBLISHING GROUP",
number = "1",

}

RIS

TY - JOUR

T1 - Endoplasmic reticulum visits highly active spines and prevents runaway potentiation of synapses

AU - Perez-Alvarez, Alberto

AU - Yin, Shuting

AU - Schulze, Christian

AU - Hammer, John A

AU - Wagner, Wolfgang

AU - Oertner, Thomas G

PY - 2020/10/8

Y1 - 2020/10/8

N2 - In hippocampal pyramidal cells, a small subset of dendritic spines contain endoplasmic reticulum (ER). In large spines, ER frequently forms a spine apparatus, while smaller spines contain just a single tubule of smooth ER. Here we show that the ER visits dendritic spines in a non-random manner, targeting spines during periods of high synaptic activity. When we blocked ER motility using a dominant negative approach against myosin V, spine synapses became stronger compared to controls. We were not able to further potentiate these maxed-out synapses, but long-term depression (LTD) was readily induced by low-frequency stimulation. We conclude that the brief ER visits to active spines have the important function of preventing runaway potentiation of individual spine synapses, keeping most of them at an intermediate strength level from which both long-term potentiation (LTP) and LTD are possible.

AB - In hippocampal pyramidal cells, a small subset of dendritic spines contain endoplasmic reticulum (ER). In large spines, ER frequently forms a spine apparatus, while smaller spines contain just a single tubule of smooth ER. Here we show that the ER visits dendritic spines in a non-random manner, targeting spines during periods of high synaptic activity. When we blocked ER motility using a dominant negative approach against myosin V, spine synapses became stronger compared to controls. We were not able to further potentiate these maxed-out synapses, but long-term depression (LTD) was readily induced by low-frequency stimulation. We conclude that the brief ER visits to active spines have the important function of preventing runaway potentiation of individual spine synapses, keeping most of them at an intermediate strength level from which both long-term potentiation (LTP) and LTD are possible.

KW - Animals

KW - Dendritic Spines/metabolism

KW - Endoplasmic Reticulum/metabolism

KW - Hippocampus/metabolism

KW - Long-Term Potentiation

KW - Myosin Type V/metabolism

KW - Rats, Wistar

KW - Synapses/metabolism

KW - Time-Lapse Imaging

U2 - 10.1038/s41467-020-18889-5

DO - 10.1038/s41467-020-18889-5

M3 - SCORING: Journal article

C2 - 33033259

VL - 11

SP - 5083

JO - NAT COMMUN

JF - NAT COMMUN

SN - 2041-1723

IS - 1

ER -