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Elevated In Vivo Levels of a Single Transcription Factor Directly Convert Satellite Glia into Oligodendrocyte-like Cells

Standard

Elevated In Vivo Levels of a Single Transcription Factor Directly Convert Satellite Glia into Oligodendrocyte-like Cells. / Weider, Matthias; Wegener, Amélie; Schmitt, Christian; Küspert, Melanie; Hillgärtner, Simone; Bösl, Michael R; Hermans-Borgmeyer, Irm; Nait-Oumesmar, Brahim; Wegner, Michael.

In: PLOS GENET, Vol. 11, No. 2, 01.02.2015, p. e1005008.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Weider, M, Wegener, A, Schmitt, C, Küspert, M, Hillgärtner, S, Bösl, MR, Hermans-Borgmeyer, I, Nait-Oumesmar, B & Wegner, M 2015, 'Elevated In Vivo Levels of a Single Transcription Factor Directly Convert Satellite Glia into Oligodendrocyte-like Cells', PLOS GENET, vol. 11, no. 2, pp. e1005008. https://doi.org/10.1371/journal.pgen.1005008

APA

Weider, M., Wegener, A., Schmitt, C., Küspert, M., Hillgärtner, S., Bösl, M. R., Hermans-Borgmeyer, I., Nait-Oumesmar, B., & Wegner, M. (2015). Elevated In Vivo Levels of a Single Transcription Factor Directly Convert Satellite Glia into Oligodendrocyte-like Cells. PLOS GENET, 11(2), e1005008. https://doi.org/10.1371/journal.pgen.1005008

Vancouver

Weider M, Wegener A, Schmitt C, Küspert M, Hillgärtner S, Bösl MR et al. Elevated In Vivo Levels of a Single Transcription Factor Directly Convert Satellite Glia into Oligodendrocyte-like Cells. PLOS GENET. 2015 Feb 1;11(2):e1005008. https://doi.org/10.1371/journal.pgen.1005008

Bibtex

@article{f8d19559037a4252a780fdd4c9b6cd52,
title = "Elevated In Vivo Levels of a Single Transcription Factor Directly Convert Satellite Glia into Oligodendrocyte-like Cells",
abstract = "Oligodendrocytes are the myelinating glia of the central nervous system and ensure rapid saltatory conduction. Shortage or loss of these cells leads to severe malfunctions as observed in human leukodystrophies and multiple sclerosis, and their replenishment by reprogramming or cell conversion strategies is an important research aim. Using a transgenic approach we increased levels of the transcription factor Sox10 throughout the mouse embryo and thereby prompted Fabp7-positive glial cells in dorsal root ganglia of the peripheral nervous system to convert into cells with oligodendrocyte characteristics including myelin gene expression. These rarely studied and poorly characterized satellite glia did not go through a classic oligodendrocyte precursor cell stage. Instead, Sox10 directly induced key elements of the regulatory network of differentiating oligodendrocytes, including Olig2, Olig1, Nkx2.2 and Myrf. An upstream enhancer mediated the direct induction of the Olig2 gene. Unlike Sox10, Olig2 was not capable of generating oligodendrocyte-like cells in dorsal root ganglia. Our findings provide proof-of-concept that Sox10 can convert conducive cells into oligodendrocyte-like cells in vivo and delineates options for future therapeutic strategies.",
author = "Matthias Weider and Am{\'e}lie Wegener and Christian Schmitt and Melanie K{\"u}spert and Simone Hillg{\"a}rtner and B{\"o}sl, {Michael R} and Irm Hermans-Borgmeyer and Brahim Nait-Oumesmar and Michael Wegner",
year = "2015",
month = feb,
day = "1",
doi = "10.1371/journal.pgen.1005008",
language = "English",
volume = "11",
pages = "e1005008",
journal = "PLOS GENET",
issn = "1553-7404",
publisher = "Public Library of Science",
number = "2",

}

RIS

TY - JOUR

T1 - Elevated In Vivo Levels of a Single Transcription Factor Directly Convert Satellite Glia into Oligodendrocyte-like Cells

AU - Weider, Matthias

AU - Wegener, Amélie

AU - Schmitt, Christian

AU - Küspert, Melanie

AU - Hillgärtner, Simone

AU - Bösl, Michael R

AU - Hermans-Borgmeyer, Irm

AU - Nait-Oumesmar, Brahim

AU - Wegner, Michael

PY - 2015/2/1

Y1 - 2015/2/1

N2 - Oligodendrocytes are the myelinating glia of the central nervous system and ensure rapid saltatory conduction. Shortage or loss of these cells leads to severe malfunctions as observed in human leukodystrophies and multiple sclerosis, and their replenishment by reprogramming or cell conversion strategies is an important research aim. Using a transgenic approach we increased levels of the transcription factor Sox10 throughout the mouse embryo and thereby prompted Fabp7-positive glial cells in dorsal root ganglia of the peripheral nervous system to convert into cells with oligodendrocyte characteristics including myelin gene expression. These rarely studied and poorly characterized satellite glia did not go through a classic oligodendrocyte precursor cell stage. Instead, Sox10 directly induced key elements of the regulatory network of differentiating oligodendrocytes, including Olig2, Olig1, Nkx2.2 and Myrf. An upstream enhancer mediated the direct induction of the Olig2 gene. Unlike Sox10, Olig2 was not capable of generating oligodendrocyte-like cells in dorsal root ganglia. Our findings provide proof-of-concept that Sox10 can convert conducive cells into oligodendrocyte-like cells in vivo and delineates options for future therapeutic strategies.

AB - Oligodendrocytes are the myelinating glia of the central nervous system and ensure rapid saltatory conduction. Shortage or loss of these cells leads to severe malfunctions as observed in human leukodystrophies and multiple sclerosis, and their replenishment by reprogramming or cell conversion strategies is an important research aim. Using a transgenic approach we increased levels of the transcription factor Sox10 throughout the mouse embryo and thereby prompted Fabp7-positive glial cells in dorsal root ganglia of the peripheral nervous system to convert into cells with oligodendrocyte characteristics including myelin gene expression. These rarely studied and poorly characterized satellite glia did not go through a classic oligodendrocyte precursor cell stage. Instead, Sox10 directly induced key elements of the regulatory network of differentiating oligodendrocytes, including Olig2, Olig1, Nkx2.2 and Myrf. An upstream enhancer mediated the direct induction of the Olig2 gene. Unlike Sox10, Olig2 was not capable of generating oligodendrocyte-like cells in dorsal root ganglia. Our findings provide proof-of-concept that Sox10 can convert conducive cells into oligodendrocyte-like cells in vivo and delineates options for future therapeutic strategies.

U2 - 10.1371/journal.pgen.1005008

DO - 10.1371/journal.pgen.1005008

M3 - SCORING: Journal article

C2 - 25680202

VL - 11

SP - e1005008

JO - PLOS GENET

JF - PLOS GENET

SN - 1553-7404

IS - 2

ER -