Efficient transformation of primary human mesenchymal stromal cells by adenovirus early region 1 oncogenes

TY - JOUR

T1 - Efficient transformation of primary human mesenchymal stromal cells by adenovirus early region 1 oncogenes

AU - Speiseder, Thomas

AU - Hofmann-Sieber, Helga

AU - Rodríguez, Estefanía

AU - Schellenberg, Anna

AU - Akyüz, Nuray

AU - Dierlamm, Judith

AU - Spruss, Thilo

AU - Lange, Claudia

AU - Dobner, Thomas

N1 - Copyright © 2016, American Society for Microbiology. All Rights Reserved.

PY - 2016/12/16

Y1 - 2016/12/16

N2 - Previous observations that human amniotic fluid cells (AFC) can be transformed by human adenovirus type 5 (HAdV-5) E1A/E1B oncogenes prompted us to identify the target cells in the AFC population that are susceptible for transformation. Our results demonstrate that one cell type corresponding to mesenchymal stem/stroma cells (hMSC), can be reproducibly transformed by HAdV-5 E1A/E1B oncogenes as efficient as primary rodent cultures. HAdV-5 E1-transformed hMSCs exhibit all properties commonly associated with a high grade of oncogenic transformation, including enhanced cell proliferation, anchorage-independent growth, increased growth rate, high telomerase activity as well as numerical and structural chromosomal aberrations. These data confirm previous work showing that HAdV preferentially transform cells of mesenchymal origin in rodents. More importantly, they demonstrate for the first time that human cells with stem cell characteristics can be completely transformed by HAdV oncogenes in tissue culture at a high efficiency. Our findings strongly support the hypothesis that undifferentiated progenitor cells or cells with stem cell-like properties are highly susceptible targets for HAdV-mediated cell transformation and suggest that virus-associated tumors in humans may originate, at least in part, from infections of these cell types. We expect that primary hMSC will replace the primary rodent cultures in HAdV viral transformation studies and are confident that these investigations will continue to uncover general principles of viral oncogenesis that can be extended to human DNA tumor viruses as well.IMPORTANCE: It is generally believed that transformation of primary human cells with HAdV-5 E1 oncogenes is very inefficient. However, a few cell lines have been successfully transformed with HAdV-5 E1A and E1B, indicating that there is a certain cell type, which is susceptible for HAdV-mediated transformation. Interestingly, all those cell lines have been derived from human embryonic tissue, albeit the exact cell type, is not known yet. We show for the first time the successful transformation of primary human mesenchymal stromal cells (hMSC) by HAdV-5 E1A and E1B. Further, we show upon HAdV-5 E1A and E1B expression, that these primary progenitor cells exhibit features of tumor cells and can no longer be differentiated into the adipogenic, chondrogenic or osteogenic lineage. Hence, primary hMSC represent a robust and novel model system to elucidate the underlying molecular mechanisms of adenovirus-mediated transformation of multipotent human progenitor cells.

AB - Previous observations that human amniotic fluid cells (AFC) can be transformed by human adenovirus type 5 (HAdV-5) E1A/E1B oncogenes prompted us to identify the target cells in the AFC population that are susceptible for transformation. Our results demonstrate that one cell type corresponding to mesenchymal stem/stroma cells (hMSC), can be reproducibly transformed by HAdV-5 E1A/E1B oncogenes as efficient as primary rodent cultures. HAdV-5 E1-transformed hMSCs exhibit all properties commonly associated with a high grade of oncogenic transformation, including enhanced cell proliferation, anchorage-independent growth, increased growth rate, high telomerase activity as well as numerical and structural chromosomal aberrations. These data confirm previous work showing that HAdV preferentially transform cells of mesenchymal origin in rodents. More importantly, they demonstrate for the first time that human cells with stem cell characteristics can be completely transformed by HAdV oncogenes in tissue culture at a high efficiency. Our findings strongly support the hypothesis that undifferentiated progenitor cells or cells with stem cell-like properties are highly susceptible targets for HAdV-mediated cell transformation and suggest that virus-associated tumors in humans may originate, at least in part, from infections of these cell types. We expect that primary hMSC will replace the primary rodent cultures in HAdV viral transformation studies and are confident that these investigations will continue to uncover general principles of viral oncogenesis that can be extended to human DNA tumor viruses as well.IMPORTANCE: It is generally believed that transformation of primary human cells with HAdV-5 E1 oncogenes is very inefficient. However, a few cell lines have been successfully transformed with HAdV-5 E1A and E1B, indicating that there is a certain cell type, which is susceptible for HAdV-mediated transformation. Interestingly, all those cell lines have been derived from human embryonic tissue, albeit the exact cell type, is not known yet. We show for the first time the successful transformation of primary human mesenchymal stromal cells (hMSC) by HAdV-5 E1A and E1B. Further, we show upon HAdV-5 E1A and E1B expression, that these primary progenitor cells exhibit features of tumor cells and can no longer be differentiated into the adipogenic, chondrogenic or osteogenic lineage. Hence, primary hMSC represent a robust and novel model system to elucidate the underlying molecular mechanisms of adenovirus-mediated transformation of multipotent human progenitor cells.

U2 - 10.1128/JVI.01782-16

DO - 10.1128/JVI.01782-16

M3 - SCORING: Journal article

C2 - 27795433

VL - 91

SP - e01782-16

JO - J VIROL

JF - J VIROL

SN - 0022-538X

IS - 1

ER -