Research ExplorerPublicationsEffect of plasma from patients with essential hypertension o...

Effect of plasma from patients with essential hypertension on vascular resistance in the isolated perfused rat kidney

Standard

Effect of plasma from patients with essential hypertension on vascular resistance in the isolated perfused rat kidney. / Bachmann, J; Schlüter, H; Storkebaum, W; Witzel, H; Wessels, F; Zidek, W.

In: CLIN SCI, Vol. 80, No. 1, 01.1991, p. 17-23.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Bachmann, J, Schlüter, H, Storkebaum, W, Witzel, H, Wessels, F & Zidek, W 1991, 'Effect of plasma from patients with essential hypertension on vascular resistance in the isolated perfused rat kidney', CLIN SCI, vol. 80, no. 1, pp. 17-23.

APA

Bachmann, J., Schlüter, H., Storkebaum, W., Witzel, H., Wessels, F., & Zidek, W. (1991). Effect of plasma from patients with essential hypertension on vascular resistance in the isolated perfused rat kidney. CLIN SCI, 80(1), 17-23.

Vancouver

Bachmann J, Schlüter H, Storkebaum W, Witzel H, Wessels F, Zidek W. Effect of plasma from patients with essential hypertension on vascular resistance in the isolated perfused rat kidney. CLIN SCI. 1991 Jan;80(1):17-23.

Bibtex

@article{c78aa0fb73c84b4e9d5600ba3a7cc044,
title = "Effect of plasma from patients with essential hypertension on vascular resistance in the isolated perfused rat kidney",
abstract = "1. Isolated perfused rat kidneys were used to study the effects of plasma fractions obtained by gel filtration from essential hypertensive patients (n = 40) and from normotensive subjects (n = 36) on resistance vessels. Perfusion pressure was recorded at a constant flow. 2. Plasma fractions were obtained by gel filtration and contained substances with a molecular mass in the range 1000-1500 Da. The plasma fractions from hypertensive patients used in this study had been shown to increase blood pressure after intravenous injection in rats. 3. In the isolated rat kidneys, the hypertensive fractions increased perfusion pressure by 20 +/- 17 mmHg (mean +/- SD, range 5-58 mmHg, n = 40). The analogous fractions from normotensive subjects did not change perfusion pressure significantly. 4. In Ca2(+)-free medium containing 2 mmol/l ethyleneglycol bis-(aminoethyl ether)tetra-acetate, the change in perfusion pressure induced by active plasma fractions was reduced by 95.2 +/- 6.3%. Addition of nifedipine to the perfusion medium reduced, but did not abolish, the pressure response of the kidneys. 5. In solutions containing phentolamine or saralasin, vasoconstriction was not reduced. 6. Thus in the active fractions from hypertensive plasma, a vasopressor agent with direct action on resistance vessels can be demonstrated. This substance probably acts by increasing Ca2+ influx in vascular smooth muscle cells.",
keywords = "Adult, Angiotensin II, Animals, Blood Pressure, Chromatography, Gel, Female, Humans, Hypertension, In Vitro Techniques, Kidney, Male, Middle Aged, Molecular Weight, Nifedipine, Norepinephrine, Phentolamine, Rats, Rats, Inbred WKY, Saralasin, Sodium-Potassium-Exchanging ATPase, Vascular Resistance, Journal Article",
author = "J Bachmann and H Schl{\"u}ter and W Storkebaum and H Witzel and F Wessels and W Zidek",
year = "1991",
month = jan,
language = "English",
volume = "80",
pages = "17--23",
journal = "CLIN SCI",
issn = "0143-5221",
publisher = "PORTLAND PRESS LTD",
number = "1",

}

RIS

TY - JOUR

T1 - Effect of plasma from patients with essential hypertension on vascular resistance in the isolated perfused rat kidney

AU - Bachmann, J

AU - Schlüter, H

AU - Storkebaum, W

AU - Witzel, H

AU - Wessels, F

AU - Zidek, W

PY - 1991/1

Y1 - 1991/1

N2 - 1. Isolated perfused rat kidneys were used to study the effects of plasma fractions obtained by gel filtration from essential hypertensive patients (n = 40) and from normotensive subjects (n = 36) on resistance vessels. Perfusion pressure was recorded at a constant flow. 2. Plasma fractions were obtained by gel filtration and contained substances with a molecular mass in the range 1000-1500 Da. The plasma fractions from hypertensive patients used in this study had been shown to increase blood pressure after intravenous injection in rats. 3. In the isolated rat kidneys, the hypertensive fractions increased perfusion pressure by 20 +/- 17 mmHg (mean +/- SD, range 5-58 mmHg, n = 40). The analogous fractions from normotensive subjects did not change perfusion pressure significantly. 4. In Ca2(+)-free medium containing 2 mmol/l ethyleneglycol bis-(aminoethyl ether)tetra-acetate, the change in perfusion pressure induced by active plasma fractions was reduced by 95.2 +/- 6.3%. Addition of nifedipine to the perfusion medium reduced, but did not abolish, the pressure response of the kidneys. 5. In solutions containing phentolamine or saralasin, vasoconstriction was not reduced. 6. Thus in the active fractions from hypertensive plasma, a vasopressor agent with direct action on resistance vessels can be demonstrated. This substance probably acts by increasing Ca2+ influx in vascular smooth muscle cells.

AB - 1. Isolated perfused rat kidneys were used to study the effects of plasma fractions obtained by gel filtration from essential hypertensive patients (n = 40) and from normotensive subjects (n = 36) on resistance vessels. Perfusion pressure was recorded at a constant flow. 2. Plasma fractions were obtained by gel filtration and contained substances with a molecular mass in the range 1000-1500 Da. The plasma fractions from hypertensive patients used in this study had been shown to increase blood pressure after intravenous injection in rats. 3. In the isolated rat kidneys, the hypertensive fractions increased perfusion pressure by 20 +/- 17 mmHg (mean +/- SD, range 5-58 mmHg, n = 40). The analogous fractions from normotensive subjects did not change perfusion pressure significantly. 4. In Ca2(+)-free medium containing 2 mmol/l ethyleneglycol bis-(aminoethyl ether)tetra-acetate, the change in perfusion pressure induced by active plasma fractions was reduced by 95.2 +/- 6.3%. Addition of nifedipine to the perfusion medium reduced, but did not abolish, the pressure response of the kidneys. 5. In solutions containing phentolamine or saralasin, vasoconstriction was not reduced. 6. Thus in the active fractions from hypertensive plasma, a vasopressor agent with direct action on resistance vessels can be demonstrated. This substance probably acts by increasing Ca2+ influx in vascular smooth muscle cells.

KW - Adult

KW - Angiotensin II

KW - Animals

KW - Blood Pressure

KW - Chromatography, Gel

KW - Female

KW - Humans

KW - Hypertension

KW - In Vitro Techniques

KW - Kidney

KW - Male

KW - Middle Aged

KW - Molecular Weight

KW - Nifedipine

KW - Norepinephrine

KW - Phentolamine

KW - Rats

KW - Rats, Inbred WKY

KW - Saralasin

KW - Sodium-Potassium-Exchanging ATPase

KW - Vascular Resistance

KW - Journal Article

M3 - SCORING: Journal article

C2 - 1846786

VL - 80

SP - 17

EP - 23

JO - CLIN SCI

JF - CLIN SCI

SN - 0143-5221

IS - 1

ER -