Effect of ozone on oral cells compared with established antimicrobials
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Effect of ozone on oral cells compared with established antimicrobials. / Huth, Karin C; Jakob, Franz M; Saugel, Bernd; Cappello, Christian; Paschos, Ekaterini; Hollweck, Regina; Hickel, Reinhard; Brand, Korbinian.
In: EUR J ORAL SCI, Vol. 114, No. 5, 01.10.2006, p. 435-40.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Effect of ozone on oral cells compared with established antimicrobials
AU - Huth, Karin C
AU - Jakob, Franz M
AU - Saugel, Bernd
AU - Cappello, Christian
AU - Paschos, Ekaterini
AU - Hollweck, Regina
AU - Hickel, Reinhard
AU - Brand, Korbinian
PY - 2006/10/1
Y1 - 2006/10/1
N2 - Ozone has been proposed as an alternative antiseptic agent in dentistry based on reports of its antimicrobial effects in both gaseous and aqueous forms. This study investigated whether gaseous ozone (4 x 10(6) microg m(-3)) and aqueous ozone (1.25-20 microg ml(-1)) exert any cytotoxic effects on human oral epithelial (BHY) cells and gingival fibroblast (HGF-1) cells compared with established antiseptics [chlorhexidine digluconate (CHX) 2%, 0.2%; sodium hypochlorite (NaOCl) 5.25%, 2.25%; hydrogen peroxide (H(2)O(2)) 3%], over a time of 1 min, and compared with the antibiotic, metronidazole, over 24 h. Cell counts, metabolic activity, Sp-1 binding, actin levels, and apoptosis were evaluated. Ozone gas was found to have toxic effects on both cell types. Essentially no cytotoxic signs were observed for aqueous ozone. CHX (2%, 0.2%) was highly toxic to BHY cells, and slightly (2%) and non-toxic (0.2%) to HGF-1 cells. NaOCl and H(2)O(2) resulted in markedly reduced cell viability (BHY, HGF-1), whereas metronidazole displayed mild toxicity only to BHY cells. Taken together, aqueous ozone revealed the highest level of biocompatibility of the tested antiseptics.
AB - Ozone has been proposed as an alternative antiseptic agent in dentistry based on reports of its antimicrobial effects in both gaseous and aqueous forms. This study investigated whether gaseous ozone (4 x 10(6) microg m(-3)) and aqueous ozone (1.25-20 microg ml(-1)) exert any cytotoxic effects on human oral epithelial (BHY) cells and gingival fibroblast (HGF-1) cells compared with established antiseptics [chlorhexidine digluconate (CHX) 2%, 0.2%; sodium hypochlorite (NaOCl) 5.25%, 2.25%; hydrogen peroxide (H(2)O(2)) 3%], over a time of 1 min, and compared with the antibiotic, metronidazole, over 24 h. Cell counts, metabolic activity, Sp-1 binding, actin levels, and apoptosis were evaluated. Ozone gas was found to have toxic effects on both cell types. Essentially no cytotoxic signs were observed for aqueous ozone. CHX (2%, 0.2%) was highly toxic to BHY cells, and slightly (2%) and non-toxic (0.2%) to HGF-1 cells. NaOCl and H(2)O(2) resulted in markedly reduced cell viability (BHY, HGF-1), whereas metronidazole displayed mild toxicity only to BHY cells. Taken together, aqueous ozone revealed the highest level of biocompatibility of the tested antiseptics.
KW - Analysis of Variance
KW - Anti-Infective Agents
KW - Apoptosis
KW - Cell Count
KW - Cells, Cultured
KW - Chlorhexidine
KW - Confidence Intervals
KW - Fibroblasts
KW - Gingiva
KW - Humans
KW - Hydrogen Peroxide
KW - Mouth Mucosa
KW - Ozone
KW - Sodium Hypochlorite
U2 - 10.1111/j.1600-0722.2006.00390.x
DO - 10.1111/j.1600-0722.2006.00390.x
M3 - SCORING: Journal article
C2 - 17026511
VL - 114
SP - 435
EP - 440
JO - EUR J ORAL SCI
JF - EUR J ORAL SCI
SN - 0909-8836
IS - 5
ER -
