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Effect of IL-15 on IgG versus IgE antibody-secreting cells in vitro

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Effect of IL-15 on IgG versus IgE antibody-secreting cells in vitro. / Esen, Meral; Forster, Johannes; Ajua, Anthony; Spänkuch, Iris; Paparoupa, Maria; Mordmüller, Benjamin; Kremsner, Peter G.

In: J IMMUNOL METHODS, Vol. 375, No. 1-2, 31.01.2012, p. 7-13.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Esen, M, Forster, J, Ajua, A, Spänkuch, I, Paparoupa, M, Mordmüller, B & Kremsner, PG 2012, 'Effect of IL-15 on IgG versus IgE antibody-secreting cells in vitro', J IMMUNOL METHODS, vol. 375, no. 1-2, pp. 7-13. https://doi.org/10.1016/j.jim.2011.08.020

APA

Esen, M., Forster, J., Ajua, A., Spänkuch, I., Paparoupa, M., Mordmüller, B., & Kremsner, P. G. (2012). Effect of IL-15 on IgG versus IgE antibody-secreting cells in vitro. J IMMUNOL METHODS, 375(1-2), 7-13. https://doi.org/10.1016/j.jim.2011.08.020

Vancouver

Esen M, Forster J, Ajua A, Spänkuch I, Paparoupa M, Mordmüller B et al. Effect of IL-15 on IgG versus IgE antibody-secreting cells in vitro. J IMMUNOL METHODS. 2012 Jan 31;375(1-2):7-13. https://doi.org/10.1016/j.jim.2011.08.020

Bibtex

@article{64d3717afd6b4d90ac6cf878743006a0,
title = "Effect of IL-15 on IgG versus IgE antibody-secreting cells in vitro",
abstract = "Immunoglobulin E (IgE) antibodies are major contributors to the pathology of atopic and allergic diseases as well as to immune response to helminth infections. Development of an adequate immunoglobulin G (IgG) immune response against infectious agents and vaccine antigens is considered in most cases as crucial for protection from disease. In vivo and in vitro production of IgE and IgG depends on cytokines and other soluble factors. Recently it has been shown that IgG antibody secreting cells (ASCs) can be generated by in vitro maturation of blood cells with Interleukin- (IL-)15 and CpG DNA or other stimulation cocktails, while IgE-ASCs develop upon cultivation with anti-CD40 and IL-4. In the present study we employed an enzyme linked immunospot assay (ELISPOT) to assess the capacity of individuals to develop into either IgE-ASCs or IgG-ASCs upon stimulation with different combinations of stimulation cocktails in order to investigate the influence of cytokines that are dysregulated in IgE-mediated immune reactions on ASC generation. Furthermore, we modified the method to assess IgG- and IgE-ASCs specific for two model antigens causing allergic rhinitis in humans. We demonstrate that IL-15, which is important for development of IgG-ASCs, decreases the number of IgE-ASCs when added to media commonly used for in vitro development of IgE-ASCs. We show that our method is suitable for the detection of specific and non-specific IgE-ASCs and IgG-ASCs and allows the investigation of the interplay between IgG-ASCs and IgE-ASCs in different populations.",
keywords = "Adult, Antibody-Producing Cells, B-Lymphocytes, Enzyme-Linked Immunospot Assay, Humans, Immunoglobulin E, Immunoglobulin G, Immunologic Factors, Interleukin-15, Interleukins, Middle Aged, Rhinitis, Allergic, Seasonal, Young Adult, Journal Article, Research Support, Non-U.S. Gov't",
author = "Meral Esen and Johannes Forster and Anthony Ajua and Iris Sp{\"a}nkuch and Maria Paparoupa and Benjamin Mordm{\"u}ller and Kremsner, {Peter G}",
note = "Copyright {\textcopyright} 2011 Elsevier B.V. All rights reserved.",
year = "2012",
month = jan,
day = "31",
doi = "10.1016/j.jim.2011.08.020",
language = "English",
volume = "375",
pages = "7--13",
journal = "J IMMUNOL METHODS",
issn = "0022-1759",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Effect of IL-15 on IgG versus IgE antibody-secreting cells in vitro

AU - Esen, Meral

AU - Forster, Johannes

AU - Ajua, Anthony

AU - Spänkuch, Iris

AU - Paparoupa, Maria

AU - Mordmüller, Benjamin

AU - Kremsner, Peter G

N1 - Copyright © 2011 Elsevier B.V. All rights reserved.

PY - 2012/1/31

Y1 - 2012/1/31

N2 - Immunoglobulin E (IgE) antibodies are major contributors to the pathology of atopic and allergic diseases as well as to immune response to helminth infections. Development of an adequate immunoglobulin G (IgG) immune response against infectious agents and vaccine antigens is considered in most cases as crucial for protection from disease. In vivo and in vitro production of IgE and IgG depends on cytokines and other soluble factors. Recently it has been shown that IgG antibody secreting cells (ASCs) can be generated by in vitro maturation of blood cells with Interleukin- (IL-)15 and CpG DNA or other stimulation cocktails, while IgE-ASCs develop upon cultivation with anti-CD40 and IL-4. In the present study we employed an enzyme linked immunospot assay (ELISPOT) to assess the capacity of individuals to develop into either IgE-ASCs or IgG-ASCs upon stimulation with different combinations of stimulation cocktails in order to investigate the influence of cytokines that are dysregulated in IgE-mediated immune reactions on ASC generation. Furthermore, we modified the method to assess IgG- and IgE-ASCs specific for two model antigens causing allergic rhinitis in humans. We demonstrate that IL-15, which is important for development of IgG-ASCs, decreases the number of IgE-ASCs when added to media commonly used for in vitro development of IgE-ASCs. We show that our method is suitable for the detection of specific and non-specific IgE-ASCs and IgG-ASCs and allows the investigation of the interplay between IgG-ASCs and IgE-ASCs in different populations.

AB - Immunoglobulin E (IgE) antibodies are major contributors to the pathology of atopic and allergic diseases as well as to immune response to helminth infections. Development of an adequate immunoglobulin G (IgG) immune response against infectious agents and vaccine antigens is considered in most cases as crucial for protection from disease. In vivo and in vitro production of IgE and IgG depends on cytokines and other soluble factors. Recently it has been shown that IgG antibody secreting cells (ASCs) can be generated by in vitro maturation of blood cells with Interleukin- (IL-)15 and CpG DNA or other stimulation cocktails, while IgE-ASCs develop upon cultivation with anti-CD40 and IL-4. In the present study we employed an enzyme linked immunospot assay (ELISPOT) to assess the capacity of individuals to develop into either IgE-ASCs or IgG-ASCs upon stimulation with different combinations of stimulation cocktails in order to investigate the influence of cytokines that are dysregulated in IgE-mediated immune reactions on ASC generation. Furthermore, we modified the method to assess IgG- and IgE-ASCs specific for two model antigens causing allergic rhinitis in humans. We demonstrate that IL-15, which is important for development of IgG-ASCs, decreases the number of IgE-ASCs when added to media commonly used for in vitro development of IgE-ASCs. We show that our method is suitable for the detection of specific and non-specific IgE-ASCs and IgG-ASCs and allows the investigation of the interplay between IgG-ASCs and IgE-ASCs in different populations.

KW - Adult

KW - Antibody-Producing Cells

KW - B-Lymphocytes

KW - Enzyme-Linked Immunospot Assay

KW - Humans

KW - Immunoglobulin E

KW - Immunoglobulin G

KW - Immunologic Factors

KW - Interleukin-15

KW - Interleukins

KW - Middle Aged

KW - Rhinitis, Allergic, Seasonal

KW - Young Adult

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.jim.2011.08.020

DO - 10.1016/j.jim.2011.08.020

M3 - SCORING: Journal article

C2 - 21945396

VL - 375

SP - 7

EP - 13

JO - J IMMUNOL METHODS

JF - J IMMUNOL METHODS

SN - 0022-1759

IS - 1-2

ER -