Research ExplorerPublicationsEffect of diadenosine polyphosphates on Ca2+ ATPase activity

Effect of diadenosine polyphosphates on Ca2+ ATPase activity

Standard

Effect of diadenosine polyphosphates on Ca2+ ATPase activity. / Jankowski, J; Schlüter, H; Tepel, M; Spieker, C; Zidek, W.

In: J MOL MED, Vol. 75, No. 9, 09.1997, p. 674-7.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Jankowski, J, Schlüter, H, Tepel, M, Spieker, C & Zidek, W 1997, 'Effect of diadenosine polyphosphates on Ca2+ ATPase activity', J MOL MED, vol. 75, no. 9, pp. 674-7.

APA

Jankowski, J., Schlüter, H., Tepel, M., Spieker, C., & Zidek, W. (1997). Effect of diadenosine polyphosphates on Ca2+ ATPase activity. J MOL MED, 75(9), 674-7.

Vancouver

Jankowski J, Schlüter H, Tepel M, Spieker C, Zidek W. Effect of diadenosine polyphosphates on Ca2+ ATPase activity. J MOL MED. 1997 Sep;75(9):674-7.

Bibtex

@article{38de3a2a0bc0460f86cbb51e88abb279,
title = "Effect of diadenosine polyphosphates on Ca2+ ATPase activity",
abstract = "Diadenosine tri-, tetra-, penta-, and hexaphosphate (Ap3A, Ap4A, Ap5A and Ap6A) have been described as having various effects on vascular tone depending on the number of phosphate groups. This study examined the effect of diadenosine polyphosphates on Ca2+ ATPase activity. The activity of the enzyme was measured spectrophotometrically as the difference in hydrolysis of ATP in the presence and absence of Ca2+ with various concentrations of ATP and diadenosine polyphosphates. The diadenosine polyphosphates increased the activity of the Ca2+ ATPase. The effect tended to be stronger with Ap5A and Ap6A than with Ap3A and Ap4A in the order of potency: Ap3A approximately AP4A < Ap5A approximately AP6A. The stimulatory effect of diadenosine polyphosphates was not competitive with that of ATP, suggesting an allosteric activation of Ca2+ ATPase by diadenosine polyphosphates. This effect may be physiologically relevant for limiting the increase in cytosolic free Ca2+ concentration elicited by diadenosine polyphosphates by receptor activation and modulating Ca2+ ATPase function under resting conditions.",
keywords = "Adenosine Triphosphate, Calcium-Transporting ATPases, Dinucleoside Phosphates, Humans, Structure-Activity Relationship, Journal Article, Research Support, Non-U.S. Gov't",
author = "J Jankowski and H Schl{\"u}ter and M Tepel and C Spieker and W Zidek",
year = "1997",
month = sep,
language = "English",
volume = "75",
pages = "674--7",
journal = "J MOL MED",
issn = "0946-2716",
publisher = "Springer",
number = "9",

}

RIS

TY - JOUR

T1 - Effect of diadenosine polyphosphates on Ca2+ ATPase activity

AU - Jankowski, J

AU - Schlüter, H

AU - Tepel, M

AU - Spieker, C

AU - Zidek, W

PY - 1997/9

Y1 - 1997/9

N2 - Diadenosine tri-, tetra-, penta-, and hexaphosphate (Ap3A, Ap4A, Ap5A and Ap6A) have been described as having various effects on vascular tone depending on the number of phosphate groups. This study examined the effect of diadenosine polyphosphates on Ca2+ ATPase activity. The activity of the enzyme was measured spectrophotometrically as the difference in hydrolysis of ATP in the presence and absence of Ca2+ with various concentrations of ATP and diadenosine polyphosphates. The diadenosine polyphosphates increased the activity of the Ca2+ ATPase. The effect tended to be stronger with Ap5A and Ap6A than with Ap3A and Ap4A in the order of potency: Ap3A approximately AP4A < Ap5A approximately AP6A. The stimulatory effect of diadenosine polyphosphates was not competitive with that of ATP, suggesting an allosteric activation of Ca2+ ATPase by diadenosine polyphosphates. This effect may be physiologically relevant for limiting the increase in cytosolic free Ca2+ concentration elicited by diadenosine polyphosphates by receptor activation and modulating Ca2+ ATPase function under resting conditions.

AB - Diadenosine tri-, tetra-, penta-, and hexaphosphate (Ap3A, Ap4A, Ap5A and Ap6A) have been described as having various effects on vascular tone depending on the number of phosphate groups. This study examined the effect of diadenosine polyphosphates on Ca2+ ATPase activity. The activity of the enzyme was measured spectrophotometrically as the difference in hydrolysis of ATP in the presence and absence of Ca2+ with various concentrations of ATP and diadenosine polyphosphates. The diadenosine polyphosphates increased the activity of the Ca2+ ATPase. The effect tended to be stronger with Ap5A and Ap6A than with Ap3A and Ap4A in the order of potency: Ap3A approximately AP4A < Ap5A approximately AP6A. The stimulatory effect of diadenosine polyphosphates was not competitive with that of ATP, suggesting an allosteric activation of Ca2+ ATPase by diadenosine polyphosphates. This effect may be physiologically relevant for limiting the increase in cytosolic free Ca2+ concentration elicited by diadenosine polyphosphates by receptor activation and modulating Ca2+ ATPase function under resting conditions.

KW - Adenosine Triphosphate

KW - Calcium-Transporting ATPases

KW - Dinucleoside Phosphates

KW - Humans

KW - Structure-Activity Relationship

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - SCORING: Journal article

C2 - 9351706

VL - 75

SP - 674

EP - 677

JO - J MOL MED

JF - J MOL MED

SN - 0946-2716

IS - 9

ER -