Early-life exposure to caffeine affects the construction and activity of cortical networks in mice
Standard
Early-life exposure to caffeine affects the construction and activity of cortical networks in mice. / Fazeli, Walid; Zappettini, Stefania; Marguet, Stephan Lawrence; Grendel, Jasper; Esclapez, Monique; Bernard, Christophe; Isbrandt, Dirk.
In: EXP NEUROL, Vol. 295, 09.2017, p. 88-103.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Early-life exposure to caffeine affects the construction and activity of cortical networks in mice
AU - Fazeli, Walid
AU - Zappettini, Stefania
AU - Marguet, Stephan Lawrence
AU - Grendel, Jasper
AU - Esclapez, Monique
AU - Bernard, Christophe
AU - Isbrandt, Dirk
N1 - Copyright © 2017 Elsevier Inc. All rights reserved.
PY - 2017/9
Y1 - 2017/9
N2 - The consumption of psychoactive drugs during pregnancy can have deleterious effects on newborns. It remains unclear whether early-life exposure to caffeine, the most widely consumed psychoactive substance, alters brain development. We hypothesized that maternal caffeine ingestion during pregnancy and the early postnatal period in mice affects the construction and activity of cortical networks in offspring. To test this hypothesis, we focused on primary visual cortex (V1) as a model neocortical region. In a study design mimicking the daily consumption of approximately three cups of coffee during pregnancy in humans, caffeine was added to the drinking water of female mice and their offspring were compared to control offspring. Caffeine altered the construction of GABAergic neuronal networks in V1, as reflected by a reduced number of somatostatin-containing GABA neurons at postnatal days 6-7, with the remaining ones showing poorly developed dendritic arbors. These findings were accompanied by increased synaptic activity in vitro and elevated network activity in vivo in V1. Similarly, in vivo hippocampal network activity was altered from the neonatal period until adulthood. Finally, caffeine-exposed offspring showed increased seizure susceptibility in a hyperthermia-induced seizure model. In summary, our results indicate detrimental effects of developmental caffeine exposure on mouse brain development.
AB - The consumption of psychoactive drugs during pregnancy can have deleterious effects on newborns. It remains unclear whether early-life exposure to caffeine, the most widely consumed psychoactive substance, alters brain development. We hypothesized that maternal caffeine ingestion during pregnancy and the early postnatal period in mice affects the construction and activity of cortical networks in offspring. To test this hypothesis, we focused on primary visual cortex (V1) as a model neocortical region. In a study design mimicking the daily consumption of approximately three cups of coffee during pregnancy in humans, caffeine was added to the drinking water of female mice and their offspring were compared to control offspring. Caffeine altered the construction of GABAergic neuronal networks in V1, as reflected by a reduced number of somatostatin-containing GABA neurons at postnatal days 6-7, with the remaining ones showing poorly developed dendritic arbors. These findings were accompanied by increased synaptic activity in vitro and elevated network activity in vivo in V1. Similarly, in vivo hippocampal network activity was altered from the neonatal period until adulthood. Finally, caffeine-exposed offspring showed increased seizure susceptibility in a hyperthermia-induced seizure model. In summary, our results indicate detrimental effects of developmental caffeine exposure on mouse brain development.
KW - Animals
KW - Animals, Newborn
KW - Caffeine
KW - Central Nervous System Stimulants
KW - Cerebral Cortex
KW - Dendrites
KW - Electrophysiological Phenomena
KW - Female
KW - In Vitro Techniques
KW - Male
KW - Mice
KW - Neocortex
KW - Nerve Net
KW - Pregnancy
KW - Prenatal Exposure Delayed Effects
KW - Seizures, Febrile
KW - Somatostatin
KW - Visual Cortex
KW - gamma-Aminobutyric Acid
KW - Journal Article
U2 - 10.1016/j.expneurol.2017.05.013
DO - 10.1016/j.expneurol.2017.05.013
M3 - SCORING: Journal article
C2 - 28576568
VL - 295
SP - 88
EP - 103
JO - EXP NEUROL
JF - EXP NEUROL
SN - 0014-4886
ER -