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Early electroretinographic features of streptozotocin-induced diabetic retinopathy

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Early electroretinographic features of streptozotocin-induced diabetic retinopathy. / Shinoda, Kei; Rejdak, Robert; Schuettauf, Frank; Blatsios, Georgios; Völker, Michael; Tanimoto, Naoyuki; Olcay, Tatar; Gekeler, Florian; Lehaci, Cristina; Naskar, Rita; Zagorski, Zbigniew; Zrenner, Eberhart.

In: CLIN EXP OPHTHALMOL, Vol. 35, No. 9, 12.2007, p. 847-54.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Shinoda, K, Rejdak, R, Schuettauf, F, Blatsios, G, Völker, M, Tanimoto, N, Olcay, T, Gekeler, F, Lehaci, C, Naskar, R, Zagorski, Z & Zrenner, E 2007, 'Early electroretinographic features of streptozotocin-induced diabetic retinopathy', CLIN EXP OPHTHALMOL, vol. 35, no. 9, pp. 847-54. https://doi.org/10.1111/j.1442-9071.2007.01607.x

APA

Shinoda, K., Rejdak, R., Schuettauf, F., Blatsios, G., Völker, M., Tanimoto, N., Olcay, T., Gekeler, F., Lehaci, C., Naskar, R., Zagorski, Z., & Zrenner, E. (2007). Early electroretinographic features of streptozotocin-induced diabetic retinopathy. CLIN EXP OPHTHALMOL, 35(9), 847-54. https://doi.org/10.1111/j.1442-9071.2007.01607.x

Vancouver

Shinoda K, Rejdak R, Schuettauf F, Blatsios G, Völker M, Tanimoto N et al. Early electroretinographic features of streptozotocin-induced diabetic retinopathy. CLIN EXP OPHTHALMOL. 2007 Dec;35(9):847-54. https://doi.org/10.1111/j.1442-9071.2007.01607.x

Bibtex

@article{91f821db61574bdb966d57550de5a382,
title = "Early electroretinographic features of streptozotocin-induced diabetic retinopathy",
abstract = "BACKGROUND: This study set out to document the early electrophysiological and immunohistochemical changes that occur in the retina of experimentally induced diabetic rats.METHODS: Diabetes was induced in rats by intraperitoneal injection of 60 mg/kg of streptozotocin (STZ). Electroretinogram readings were taken monthly under either short-duration or long-duration stimuli for up to 3 months after STZ. Oscillatory potentials (OP) and the amplitudes and implicit times of a- and b-waves were analysed, and b-wave amplitudes were analysed using a Naka-Rushton fit. Scotopic a-waves were analysed with photoreceptor models, and Rmp3 (the maximum a-wave amplitude) and S (sensitivity) were calculated. Three months after STZ injection, immunohistochemistry for glial fibrillary acidic protein was performed on the retinas of the STZ-treated rats and age-matched controls.RESULTS: The implicit OP times were significantly longer in the diabetic rats as compared with the controls, and this difference was noted as early as 1 month following STZ treatment. Other electrophysiological parameters, such as OP amplitudes, a- and b-wave amplitude as well as the implicit times, did not differ from controls at this stage. The sacrificed STZ-treated rats also demonstrated marked enhancement of glial fibrillary acidic protein immunoreactivity, suggesting that at least in experimentally induced diabetic retinopathy there is increased M{\"u}ller cell reactivity.CONCLUSION: The results of this study indicated that functional alterations in the retina develop rapidly after the onset of diabetes. Analysis of each electroretinogram component may be useful in further investigating the development mechanisms of diabetic retinopathy.",
keywords = "Animals, Diabetes Mellitus, Experimental/complications, Diabetic Retinopathy/diagnosis, Electroretinography, Glial Fibrillary Acidic Protein/metabolism, Immunohistochemistry/methods, Male, Rats, Rats, Inbred BN, Retina/metabolism, Time Factors, Tissue Distribution",
author = "Kei Shinoda and Robert Rejdak and Frank Schuettauf and Georgios Blatsios and Michael V{\"o}lker and Naoyuki Tanimoto and Tatar Olcay and Florian Gekeler and Cristina Lehaci and Rita Naskar and Zbigniew Zagorski and Eberhart Zrenner",
year = "2007",
month = dec,
doi = "10.1111/j.1442-9071.2007.01607.x",
language = "English",
volume = "35",
pages = "847--54",
journal = "CLIN EXP OPHTHALMOL",
issn = "1442-6404",
publisher = "Wiley-Blackwell",
number = "9",

}

RIS

TY - JOUR

T1 - Early electroretinographic features of streptozotocin-induced diabetic retinopathy

AU - Shinoda, Kei

AU - Rejdak, Robert

AU - Schuettauf, Frank

AU - Blatsios, Georgios

AU - Völker, Michael

AU - Tanimoto, Naoyuki

AU - Olcay, Tatar

AU - Gekeler, Florian

AU - Lehaci, Cristina

AU - Naskar, Rita

AU - Zagorski, Zbigniew

AU - Zrenner, Eberhart

PY - 2007/12

Y1 - 2007/12

N2 - BACKGROUND: This study set out to document the early electrophysiological and immunohistochemical changes that occur in the retina of experimentally induced diabetic rats.METHODS: Diabetes was induced in rats by intraperitoneal injection of 60 mg/kg of streptozotocin (STZ). Electroretinogram readings were taken monthly under either short-duration or long-duration stimuli for up to 3 months after STZ. Oscillatory potentials (OP) and the amplitudes and implicit times of a- and b-waves were analysed, and b-wave amplitudes were analysed using a Naka-Rushton fit. Scotopic a-waves were analysed with photoreceptor models, and Rmp3 (the maximum a-wave amplitude) and S (sensitivity) were calculated. Three months after STZ injection, immunohistochemistry for glial fibrillary acidic protein was performed on the retinas of the STZ-treated rats and age-matched controls.RESULTS: The implicit OP times were significantly longer in the diabetic rats as compared with the controls, and this difference was noted as early as 1 month following STZ treatment. Other electrophysiological parameters, such as OP amplitudes, a- and b-wave amplitude as well as the implicit times, did not differ from controls at this stage. The sacrificed STZ-treated rats also demonstrated marked enhancement of glial fibrillary acidic protein immunoreactivity, suggesting that at least in experimentally induced diabetic retinopathy there is increased Müller cell reactivity.CONCLUSION: The results of this study indicated that functional alterations in the retina develop rapidly after the onset of diabetes. Analysis of each electroretinogram component may be useful in further investigating the development mechanisms of diabetic retinopathy.

AB - BACKGROUND: This study set out to document the early electrophysiological and immunohistochemical changes that occur in the retina of experimentally induced diabetic rats.METHODS: Diabetes was induced in rats by intraperitoneal injection of 60 mg/kg of streptozotocin (STZ). Electroretinogram readings were taken monthly under either short-duration or long-duration stimuli for up to 3 months after STZ. Oscillatory potentials (OP) and the amplitudes and implicit times of a- and b-waves were analysed, and b-wave amplitudes were analysed using a Naka-Rushton fit. Scotopic a-waves were analysed with photoreceptor models, and Rmp3 (the maximum a-wave amplitude) and S (sensitivity) were calculated. Three months after STZ injection, immunohistochemistry for glial fibrillary acidic protein was performed on the retinas of the STZ-treated rats and age-matched controls.RESULTS: The implicit OP times were significantly longer in the diabetic rats as compared with the controls, and this difference was noted as early as 1 month following STZ treatment. Other electrophysiological parameters, such as OP amplitudes, a- and b-wave amplitude as well as the implicit times, did not differ from controls at this stage. The sacrificed STZ-treated rats also demonstrated marked enhancement of glial fibrillary acidic protein immunoreactivity, suggesting that at least in experimentally induced diabetic retinopathy there is increased Müller cell reactivity.CONCLUSION: The results of this study indicated that functional alterations in the retina develop rapidly after the onset of diabetes. Analysis of each electroretinogram component may be useful in further investigating the development mechanisms of diabetic retinopathy.

KW - Animals

KW - Diabetes Mellitus, Experimental/complications

KW - Diabetic Retinopathy/diagnosis

KW - Electroretinography

KW - Glial Fibrillary Acidic Protein/metabolism

KW - Immunohistochemistry/methods

KW - Male

KW - Rats

KW - Rats, Inbred BN

KW - Retina/metabolism

KW - Time Factors

KW - Tissue Distribution

U2 - 10.1111/j.1442-9071.2007.01607.x

DO - 10.1111/j.1442-9071.2007.01607.x

M3 - SCORING: Journal article

C2 - 18173414

VL - 35

SP - 847

EP - 854

JO - CLIN EXP OPHTHALMOL

JF - CLIN EXP OPHTHALMOL

SN - 1442-6404

IS - 9

ER -