Down-regulation of CD73 on B cells of patients with viremic HIV correlates with B cell activation and disease progression
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Down-regulation of CD73 on B cells of patients with viremic HIV correlates with B cell activation and disease progression. / Kim, Eun-Seong; Ackermann, Christin; Tóth, Ilona; Dierks, Patrick; Eberhard, Johanna M; Wroblewski, Raluca; Scherg, Felix; Geyer, Matthias; Schmidt, Reinhold E; Beisel, Claudia; Bockhorn, Maximilian; Haag, Friedrich; Lunzen, Jan Van; Schulze Zur Wiesch, Julian.
In: J LEUKOCYTE BIOL, Vol. 101, No. 5, 05.2017, p. 1263-1271.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Down-regulation of CD73 on B cells of patients with viremic HIV correlates with B cell activation and disease progression
AU - Kim, Eun-Seong
AU - Ackermann, Christin
AU - Tóth, Ilona
AU - Dierks, Patrick
AU - Eberhard, Johanna M
AU - Wroblewski, Raluca
AU - Scherg, Felix
AU - Geyer, Matthias
AU - Schmidt, Reinhold E
AU - Beisel, Claudia
AU - Bockhorn, Maximilian
AU - Haag, Friedrich
AU - Lunzen, Jan Van
AU - Schulze Zur Wiesch, Julian
N1 - © Society for Leukocyte Biology.
PY - 2017/5
Y1 - 2017/5
N2 - Recently, alterations of the T cell expression of the ectonucleotidases, CD39 and CD73, during HIV infection have been described. Here, peripheral (n = 70) and lymph nodal B cells (n = 10) of patients with HIV at different stages of disease as well as uninfected individuals were analyzed via multicolor flow cytometry with regard to expression of CD39 and CD73 and differentiation, proliferation, and exhaustion status. Patients with chronic, untreated HIV showed a significantly decreased frequency of CD73-expressing B cells (P < 0.001) compared with healthy controls. Decreased frequencies of CD39(+)CD73(+) B cells in patients with HIV correlated with low CD4(+) counts (P < 0.0256) as well as increased proliferation and exhaustion status as determined by Ki-67 and programmed death-1 expression. Down-regulation of CD73 was observed in naive and memory B cells as determined by CD27 and CD21. Neither HIV elite controller patients nor antiretroviral therapy-treated patients had significantly lower CD39 and CD73 expression on B cells compared with healthy controls. Of importance, low CD73(+) expression on B cells was associated with modulated in vitro B cell function. Further in vivo studies are warranted to evaluate the in vivo role of phenotypic loss of CD73 in B cell dysregulation in HIV.
AB - Recently, alterations of the T cell expression of the ectonucleotidases, CD39 and CD73, during HIV infection have been described. Here, peripheral (n = 70) and lymph nodal B cells (n = 10) of patients with HIV at different stages of disease as well as uninfected individuals were analyzed via multicolor flow cytometry with regard to expression of CD39 and CD73 and differentiation, proliferation, and exhaustion status. Patients with chronic, untreated HIV showed a significantly decreased frequency of CD73-expressing B cells (P < 0.001) compared with healthy controls. Decreased frequencies of CD39(+)CD73(+) B cells in patients with HIV correlated with low CD4(+) counts (P < 0.0256) as well as increased proliferation and exhaustion status as determined by Ki-67 and programmed death-1 expression. Down-regulation of CD73 was observed in naive and memory B cells as determined by CD27 and CD21. Neither HIV elite controller patients nor antiretroviral therapy-treated patients had significantly lower CD39 and CD73 expression on B cells compared with healthy controls. Of importance, low CD73(+) expression on B cells was associated with modulated in vitro B cell function. Further in vivo studies are warranted to evaluate the in vivo role of phenotypic loss of CD73 in B cell dysregulation in HIV.
KW - Journal Article
U2 - 10.1189/jlb.5A0816-346R
DO - 10.1189/jlb.5A0816-346R
M3 - SCORING: Journal article
C2 - 28193736
VL - 101
SP - 1263
EP - 1271
JO - J LEUKOCYTE BIOL
JF - J LEUKOCYTE BIOL
SN - 0741-5400
IS - 5
ER -
