Distribution of the two forms of Plasmodium falciparum erythrocyte binding antigen-175 (eba-175) gene in Lao PDR

TY - JOUR

T1 - Distribution of the two forms of Plasmodium falciparum erythrocyte binding antigen-175 (eba-175) gene in Lao PDR

AU - Dittrich, Sabine

AU - Schwöbel, Babett

AU - Jordan, Sabine

AU - Vanisaveth, Viengsay

AU - Rattanaxay, Phetsouvanh

AU - Christophel, Eva-Maria

AU - Phompida, Samlane

AU - Jelinek, Tomas

PY - 2003/7/22

Y1 - 2003/7/22

N2 - BACKGROUND: The erythrocyte binding antigen 175 (EBA-175) is a 175 kDa antigen of Plasmodium falciparum and plays a major role in erythrocyte recognition by the parasite. The antigen is also supposed to be partly responsible for the invasion of erythrocytes by merozoites. EBA-175 has been sequenced from the FCR-3 and CAMP strains of P. falciparum. The sequences were identical in most parts of the gene. Differences were apparent in a 423 bp segment in the FCR-3 strain, the F-Fragment, that is not found in the CAMP-strain and a 342 bp segment, the C-Fragment, which is present in the CAMP-strain but not in the FCR-3-strain. The aim of this study was to assess the distribution of the two EBA-175-alleles in the Lao PDR.MATERIALS & METHODS: Altogether, 240 blood-samples were collected in two areas of the country: Attapeu in the south and Lung Namtha in the north. Subsequently, the material was scanned for the F-and C-fragments.RESULTS: In the whole study population, 52% carried the F-fragment, and 41% the C-fragment while seven percent of the patients were infected with at least two parasite strains and showed both alleles.CONCLUSION: Distribution of the alleles showed significant differences between the north and the south province. Reasons for this include possible importation of different parasite strains from neighbouring countries.

AB - BACKGROUND: The erythrocyte binding antigen 175 (EBA-175) is a 175 kDa antigen of Plasmodium falciparum and plays a major role in erythrocyte recognition by the parasite. The antigen is also supposed to be partly responsible for the invasion of erythrocytes by merozoites. EBA-175 has been sequenced from the FCR-3 and CAMP strains of P. falciparum. The sequences were identical in most parts of the gene. Differences were apparent in a 423 bp segment in the FCR-3 strain, the F-Fragment, that is not found in the CAMP-strain and a 342 bp segment, the C-Fragment, which is present in the CAMP-strain but not in the FCR-3-strain. The aim of this study was to assess the distribution of the two EBA-175-alleles in the Lao PDR.MATERIALS & METHODS: Altogether, 240 blood-samples were collected in two areas of the country: Attapeu in the south and Lung Namtha in the north. Subsequently, the material was scanned for the F-and C-fragments.RESULTS: In the whole study population, 52% carried the F-fragment, and 41% the C-fragment while seven percent of the patients were infected with at least two parasite strains and showed both alleles.CONCLUSION: Distribution of the alleles showed significant differences between the north and the south province. Reasons for this include possible importation of different parasite strains from neighbouring countries.

KW - Adolescent

KW - Adult

KW - Aged

KW - Animals

KW - Antigens, Protozoan/genetics

KW - Carrier Proteins/genetics

KW - Child

KW - Child, Preschool

KW - Female

KW - Humans

KW - Infant

KW - Laos/epidemiology

KW - Malaria, Falciparum/blood

KW - Male

KW - Middle Aged

KW - Parasitemia/epidemiology

KW - Peptide Fragments/genetics

KW - Plasmodium falciparum/chemistry

KW - Protein Isoforms/genetics

KW - Protozoan Proteins/genetics

KW - Treatment Outcome

U2 - 10.1186/1475-2875-2-23

DO - 10.1186/1475-2875-2-23

M3 - SCORING: Journal article

C2 - 12901736

VL - 2

SP - 23

JO - MALARIA J

JF - MALARIA J

SN - 1475-2875

ER -