Distinct gene expression profiles: nodal versus extranodal diffuse large B-cell lymphoma.
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Distinct gene expression profiles: nodal versus extranodal diffuse large B-cell lymphoma. / Jehan, Zeenath; Siraj, Abdul K; Abubaker, Jehad; Ruiz, Christian; Simon, Ronald; Sultana, Mehar; Uddin, Shahab; Bavi, Prashant; Hussain, Azhar; Razack, Shirin; Ezzat, Adnan; Al-Dayel, Fouad; Sauter, Guido; Al-Kuraya, Khawla S.
In: ONCOLOGY-BASEL, Vol. 75, No. 1-2, 1-2, 2008, p. 71-80.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Distinct gene expression profiles: nodal versus extranodal diffuse large B-cell lymphoma.
AU - Jehan, Zeenath
AU - Siraj, Abdul K
AU - Abubaker, Jehad
AU - Ruiz, Christian
AU - Simon, Ronald
AU - Sultana, Mehar
AU - Uddin, Shahab
AU - Bavi, Prashant
AU - Hussain, Azhar
AU - Razack, Shirin
AU - Ezzat, Adnan
AU - Al-Dayel, Fouad
AU - Sauter, Guido
AU - Al-Kuraya, Khawla S
PY - 2008
Y1 - 2008
N2 - INTRODUCTION: Approximately one third of diffuse large B-cell lymphomas (DLBCL) arises from tissues different from the lymph node. Perceived differences in outcome between extranodal and nodal DLBCL raise the possibility that these subgroups may represent different biological and clinical entities. METHODS: Microarray GeneChip technology was used for global gene expression profiles from nodal (n = 19) and extranodal (n = 8) DLBCL, to examine possible differences between these subgroups. Quantitative RT-PCR was employed for validation of microarray data. Differential expression levels of p16 (CDKN2A) were confirmed by means of immunohistochemistry on a tissue microarray comprising more than 200 lymphoma samples. RESULTS: A total of 218, over (124)- and underexpressed (94) genes were found to be differentially expressed in extranodal DLBCL compared with nodal DLBCL, including cytokines/chemokines, chromosome-replication-related genes and DNA repair genes. Quantitative RT-PCR confirmed the microarray data. A higher rate of p16 positivity was found in extranodal lymphomas. However, prognostic importance of p16 was associated with nodal rather than extranodal lymphomas. CONCLUSION: Our data suggest that a better distinction of these subgroups based on molecular classifiers is feasible and may greatly facilitate the determination of specific relevant clinical features and therapeutic implications of DLBCL with primary extranodal or nodal location.
AB - INTRODUCTION: Approximately one third of diffuse large B-cell lymphomas (DLBCL) arises from tissues different from the lymph node. Perceived differences in outcome between extranodal and nodal DLBCL raise the possibility that these subgroups may represent different biological and clinical entities. METHODS: Microarray GeneChip technology was used for global gene expression profiles from nodal (n = 19) and extranodal (n = 8) DLBCL, to examine possible differences between these subgroups. Quantitative RT-PCR was employed for validation of microarray data. Differential expression levels of p16 (CDKN2A) were confirmed by means of immunohistochemistry on a tissue microarray comprising more than 200 lymphoma samples. RESULTS: A total of 218, over (124)- and underexpressed (94) genes were found to be differentially expressed in extranodal DLBCL compared with nodal DLBCL, including cytokines/chemokines, chromosome-replication-related genes and DNA repair genes. Quantitative RT-PCR confirmed the microarray data. A higher rate of p16 positivity was found in extranodal lymphomas. However, prognostic importance of p16 was associated with nodal rather than extranodal lymphomas. CONCLUSION: Our data suggest that a better distinction of these subgroups based on molecular classifiers is feasible and may greatly facilitate the determination of specific relevant clinical features and therapeutic implications of DLBCL with primary extranodal or nodal location.
M3 - SCORING: Zeitschriftenaufsatz
VL - 75
SP - 71
EP - 80
JO - ONCOLOGY-BASEL
JF - ONCOLOGY-BASEL
SN - 0030-2414
IS - 1-2
M1 - 1-2
ER -
