Disease-Linked Glutarylation Impairs Function and Interactions of Mitochondrial Proteins and Contributes to Mitochondrial Heterogeneity
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Disease-Linked Glutarylation Impairs Function and Interactions of Mitochondrial Proteins and Contributes to Mitochondrial Heterogeneity. / Schmiesing, Jessica; Storch, Stephan; Dörfler, Ann-Cathrin; Schweizer, Michaela; Makrypidi-Fraune, Georgia; Thelen, Melanie; Sylvester, Marc; Gieselmann, Volkmar; Meyer-Schwesinger, Catherine; Koch-Nolte, Friedrich; Tidow, Henning; Mühlhausen, Chris; Waheed, Abdul; Sly, William S; Braulke, Thomas.
In: CELL REP, Vol. 24, No. 11, 11.09.2018, p. 2946-2956.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Disease-Linked Glutarylation Impairs Function and Interactions of Mitochondrial Proteins and Contributes to Mitochondrial Heterogeneity
AU - Schmiesing, Jessica
AU - Storch, Stephan
AU - Dörfler, Ann-Cathrin
AU - Schweizer, Michaela
AU - Makrypidi-Fraune, Georgia
AU - Thelen, Melanie
AU - Sylvester, Marc
AU - Gieselmann, Volkmar
AU - Meyer-Schwesinger, Catherine
AU - Koch-Nolte, Friedrich
AU - Tidow, Henning
AU - Mühlhausen, Chris
AU - Waheed, Abdul
AU - Sly, William S
AU - Braulke, Thomas
N1 - Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.
PY - 2018/9/11
Y1 - 2018/9/11
N2 - Lysine glutarylation (Kglu) of mitochondrial proteins is associated with glutaryl-CoA dehydrogenase (GCDH) deficiency, which impairs lysine/tryptophan degradation and causes destruction of striatal neurons during catabolic crisis with subsequent movement disability. By investigating the role of Kglu modifications in this disease, we compared the brain and liver glutarylomes of Gcdh-deficient mice. In the brain, we identified 73 Kglu sites on 37 mitochondrial proteins involved in various metabolic degradation pathways. Ultrastructural immunogold studies indicated that glutarylated proteins are heterogeneously distributed in mitochondria, which are exclusively localized in glial cells. In liver cells, all mitochondria contain Kglu-modified proteins. Glutarylation reduces the catalytic activities of the most abundant glutamate dehydrogenase (GDH) and the brain-specific carbonic anhydrase 5b and interferes with GDH-protein interactions. We propose that Kglu contributes to the functional heterogeneity of mitochondria and may metabolically adapt glial cells to the activity and metabolic demands of neighboring GCDH-deficient neurons.
AB - Lysine glutarylation (Kglu) of mitochondrial proteins is associated with glutaryl-CoA dehydrogenase (GCDH) deficiency, which impairs lysine/tryptophan degradation and causes destruction of striatal neurons during catabolic crisis with subsequent movement disability. By investigating the role of Kglu modifications in this disease, we compared the brain and liver glutarylomes of Gcdh-deficient mice. In the brain, we identified 73 Kglu sites on 37 mitochondrial proteins involved in various metabolic degradation pathways. Ultrastructural immunogold studies indicated that glutarylated proteins are heterogeneously distributed in mitochondria, which are exclusively localized in glial cells. In liver cells, all mitochondria contain Kglu-modified proteins. Glutarylation reduces the catalytic activities of the most abundant glutamate dehydrogenase (GDH) and the brain-specific carbonic anhydrase 5b and interferes with GDH-protein interactions. We propose that Kglu contributes to the functional heterogeneity of mitochondria and may metabolically adapt glial cells to the activity and metabolic demands of neighboring GCDH-deficient neurons.
KW - Journal Article
U2 - 10.1016/j.celrep.2018.08.014
DO - 10.1016/j.celrep.2018.08.014
M3 - SCORING: Journal article
C2 - 30208319
VL - 24
SP - 2946
EP - 2956
JO - CELL REP
JF - CELL REP
SN - 2211-1247
IS - 11
ER -