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Disease-associated genotypes of the commensal skin bacterium Staphylococcus epidermidis

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Disease-associated genotypes of the commensal skin bacterium Staphylococcus epidermidis. / Méric, Guillaume; Mageiros, Leonardos; Pensar, Johan; Laabei, Maisem; Yahara, Koji; Pascoe, Ben; Kittiwan, Nattinee; Tadee, Phacharaporn; Post, Virginia; Lamble, Sarah; Bowden, Rory; Bray, James E; Morgenstern, Mario; Jolley, Keith A; Maiden, Martin C J; Feil, Edward J; Didelot, Xavier; Miragaia, Maria; de Lencastre, Herminia; Moriarty, T Fintan; Rohde, Holger; Massey, Ruth; Mack, Dietrich; Corander, Jukka; Sheppard, Samuel K.

In: NAT COMMUN, Vol. 9, 28.11.2018, p. 5034.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Méric, G, Mageiros, L, Pensar, J, Laabei, M, Yahara, K, Pascoe, B, Kittiwan, N, Tadee, P, Post, V, Lamble, S, Bowden, R, Bray, JE, Morgenstern, M, Jolley, KA, Maiden, MCJ, Feil, EJ, Didelot, X, Miragaia, M, de Lencastre, H, Moriarty, TF, Rohde, H, Massey, R, Mack, D, Corander, J & Sheppard, SK 2018, 'Disease-associated genotypes of the commensal skin bacterium Staphylococcus epidermidis', NAT COMMUN, vol. 9, pp. 5034. https://doi.org/10.1038/s41467-018-07368-7

APA

Méric, G., Mageiros, L., Pensar, J., Laabei, M., Yahara, K., Pascoe, B., Kittiwan, N., Tadee, P., Post, V., Lamble, S., Bowden, R., Bray, J. E., Morgenstern, M., Jolley, K. A., Maiden, M. C. J., Feil, E. J., Didelot, X., Miragaia, M., de Lencastre, H., ... Sheppard, S. K. (2018). Disease-associated genotypes of the commensal skin bacterium Staphylococcus epidermidis. NAT COMMUN, 9, 5034. https://doi.org/10.1038/s41467-018-07368-7

Vancouver

Méric G, Mageiros L, Pensar J, Laabei M, Yahara K, Pascoe B et al. Disease-associated genotypes of the commensal skin bacterium Staphylococcus epidermidis. NAT COMMUN. 2018 Nov 28;9:5034. https://doi.org/10.1038/s41467-018-07368-7

Bibtex

@article{39eeb2fe647f4f9d9aca393e08b620ba,
title = "Disease-associated genotypes of the commensal skin bacterium Staphylococcus epidermidis",
abstract = "Some of the most common infectious diseases are caused by bacteria that naturally colonise humans asymptomatically. Combating these opportunistic pathogens requires an understanding of the traits that differentiate infecting strains from harmless relatives. Staphylococcus epidermidis is carried asymptomatically on the skin and mucous membranes of virtually all humans but is a major cause of nosocomial infection associated with invasive procedures. Here we address the underlying evolutionary mechanisms of opportunistic pathogenicity by combining pangenome-wide association studies and laboratory microbiology to compare S. epidermidis from bloodstream and wound infections and asymptomatic carriage. We identify 61 genes containing infection-associated genetic elements (k-mers) that correlate with in vitro variation in known pathogenicity traits (biofilm formation, cell toxicity, interleukin-8 production, methicillin resistance). Horizontal gene transfer spreads these elements, allowing divergent clones to cause infection. Finally, Random Forest model prediction of disease status (carriage vs. infection) identifies pathogenicity elements in 415 S. epidermidis isolates with 80% accuracy, demonstrating the potential for identifying risk genotypes pre-operatively.",
keywords = "Journal Article",
author = "Guillaume M{\'e}ric and Leonardos Mageiros and Johan Pensar and Maisem Laabei and Koji Yahara and Ben Pascoe and Nattinee Kittiwan and Phacharaporn Tadee and Virginia Post and Sarah Lamble and Rory Bowden and Bray, {James E} and Mario Morgenstern and Jolley, {Keith A} and Maiden, {Martin C J} and Feil, {Edward J} and Xavier Didelot and Maria Miragaia and {de Lencastre}, Herminia and Moriarty, {T Fintan} and Holger Rohde and Ruth Massey and Dietrich Mack and Jukka Corander and Sheppard, {Samuel K}",
year = "2018",
month = nov,
day = "28",
doi = "10.1038/s41467-018-07368-7",
language = "English",
volume = "9",
pages = "5034",
journal = "NAT COMMUN",
issn = "2041-1723",
publisher = "NATURE PUBLISHING GROUP",

}

RIS

TY - JOUR

T1 - Disease-associated genotypes of the commensal skin bacterium Staphylococcus epidermidis

AU - Méric, Guillaume

AU - Mageiros, Leonardos

AU - Pensar, Johan

AU - Laabei, Maisem

AU - Yahara, Koji

AU - Pascoe, Ben

AU - Kittiwan, Nattinee

AU - Tadee, Phacharaporn

AU - Post, Virginia

AU - Lamble, Sarah

AU - Bowden, Rory

AU - Bray, James E

AU - Morgenstern, Mario

AU - Jolley, Keith A

AU - Maiden, Martin C J

AU - Feil, Edward J

AU - Didelot, Xavier

AU - Miragaia, Maria

AU - de Lencastre, Herminia

AU - Moriarty, T Fintan

AU - Rohde, Holger

AU - Massey, Ruth

AU - Mack, Dietrich

AU - Corander, Jukka

AU - Sheppard, Samuel K

PY - 2018/11/28

Y1 - 2018/11/28

N2 - Some of the most common infectious diseases are caused by bacteria that naturally colonise humans asymptomatically. Combating these opportunistic pathogens requires an understanding of the traits that differentiate infecting strains from harmless relatives. Staphylococcus epidermidis is carried asymptomatically on the skin and mucous membranes of virtually all humans but is a major cause of nosocomial infection associated with invasive procedures. Here we address the underlying evolutionary mechanisms of opportunistic pathogenicity by combining pangenome-wide association studies and laboratory microbiology to compare S. epidermidis from bloodstream and wound infections and asymptomatic carriage. We identify 61 genes containing infection-associated genetic elements (k-mers) that correlate with in vitro variation in known pathogenicity traits (biofilm formation, cell toxicity, interleukin-8 production, methicillin resistance). Horizontal gene transfer spreads these elements, allowing divergent clones to cause infection. Finally, Random Forest model prediction of disease status (carriage vs. infection) identifies pathogenicity elements in 415 S. epidermidis isolates with 80% accuracy, demonstrating the potential for identifying risk genotypes pre-operatively.

AB - Some of the most common infectious diseases are caused by bacteria that naturally colonise humans asymptomatically. Combating these opportunistic pathogens requires an understanding of the traits that differentiate infecting strains from harmless relatives. Staphylococcus epidermidis is carried asymptomatically on the skin and mucous membranes of virtually all humans but is a major cause of nosocomial infection associated with invasive procedures. Here we address the underlying evolutionary mechanisms of opportunistic pathogenicity by combining pangenome-wide association studies and laboratory microbiology to compare S. epidermidis from bloodstream and wound infections and asymptomatic carriage. We identify 61 genes containing infection-associated genetic elements (k-mers) that correlate with in vitro variation in known pathogenicity traits (biofilm formation, cell toxicity, interleukin-8 production, methicillin resistance). Horizontal gene transfer spreads these elements, allowing divergent clones to cause infection. Finally, Random Forest model prediction of disease status (carriage vs. infection) identifies pathogenicity elements in 415 S. epidermidis isolates with 80% accuracy, demonstrating the potential for identifying risk genotypes pre-operatively.

KW - Journal Article

U2 - 10.1038/s41467-018-07368-7

DO - 10.1038/s41467-018-07368-7

M3 - SCORING: Journal article

C2 - 30487573

VL - 9

SP - 5034

JO - NAT COMMUN

JF - NAT COMMUN

SN - 2041-1723

ER -