Discussion of the applicability of microarrays: profiling of leukemias.
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Discussion of the applicability of microarrays: profiling of leukemias. / Haferlach, Torsten; Bacher, Ulrike; Kohlmann, Alexander; Haferlach, Claudia.
In: Methods Mol Biol, Vol. 509, 2009, p. 15-33.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Discussion of the applicability of microarrays: profiling of leukemias.
AU - Haferlach, Torsten
AU - Bacher, Ulrike
AU - Kohlmann, Alexander
AU - Haferlach, Claudia
PY - 2009
Y1 - 2009
N2 - Leukemias are classified according to clinical, morphologic, and immunologic phenotypes, caused by specific genetic aberrations in association to distinct prognostic profiles. Usually the subtypes are defined using complementary laboratory methods, such as multiparameter flow cytometry, cytogenetics in combination with fluorescence in situ hybridization, and molecular methods such as the polymerase chain reaction. The genetic variations of the different subtypes lead to distinct changes also in gene expression, which is comprehensively analysed by DNA microarrays. Thus, first gene expression profiling studies showed that analysis with whole-genome DNA microarrays leads to a prediction accuracy of 95.6% with respect to the classical methods, and even allowed a further distinction of subtypes. It is expected that diagnostic strategies can be optimized with this new technology and that the understanding of the molecular pathogenesis of leukemias will be significantly improved. This could also lead to the identification of new targets for future drugs.
AB - Leukemias are classified according to clinical, morphologic, and immunologic phenotypes, caused by specific genetic aberrations in association to distinct prognostic profiles. Usually the subtypes are defined using complementary laboratory methods, such as multiparameter flow cytometry, cytogenetics in combination with fluorescence in situ hybridization, and molecular methods such as the polymerase chain reaction. The genetic variations of the different subtypes lead to distinct changes also in gene expression, which is comprehensively analysed by DNA microarrays. Thus, first gene expression profiling studies showed that analysis with whole-genome DNA microarrays leads to a prediction accuracy of 95.6% with respect to the classical methods, and even allowed a further distinction of subtypes. It is expected that diagnostic strategies can be optimized with this new technology and that the understanding of the molecular pathogenesis of leukemias will be significantly improved. This could also lead to the identification of new targets for future drugs.
M3 - SCORING: Zeitschriftenaufsatz
VL - 509
SP - 15
EP - 33
JO - Methods Mol Biol
JF - Methods Mol Biol
SN - 1064-3745
ER -
