Direct detection of Anaplasma phagocytophilum by polymerase chain reaction followed by electrospray ionization mass spectrometry from human blood
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Direct detection of Anaplasma phagocytophilum by polymerase chain reaction followed by electrospray ionization mass spectrometry from human blood. / Lagler, Heimo; Harrison, Nicole; Kussmann, Manuel; Obermüller, Markus; Burgmann, Heinz; Makristathis, Athanasios; Ramharter, Michael.
In: INT J INFECT DIS, Vol. 60, 07.2017, p. 61-63.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Direct detection of Anaplasma phagocytophilum by polymerase chain reaction followed by electrospray ionization mass spectrometry from human blood
AU - Lagler, Heimo
AU - Harrison, Nicole
AU - Kussmann, Manuel
AU - Obermüller, Markus
AU - Burgmann, Heinz
AU - Makristathis, Athanasios
AU - Ramharter, Michael
N1 - Copyright © 2017 The Author(s). Published by Elsevier Ltd.. All rights reserved.
PY - 2017/7
Y1 - 2017/7
N2 - Bacterial pathogens not detectable via commercial blood culture assays represent an important challenge for infectious disease physicians, in particular if clinical symptoms of the illness are non-specific. In this report, Anaplasma phagocytophilum was detected directly in a peripheral blood sample from a febrile patient reporting a tick bite. This was done using a commercial system based on PCR followed by electrospray ionization mass spectrometry (ESI-MS). The diagnosis of a human granulocytic anaplasmosis infection was established using this diagnostic methodology for the first time. Human granulocytic anaplasmosis is a neglected zoonotic disease in Europe. Its seroprevalence is similar in North America and Europe, but in contrast to the USA, it is rarely diagnosed in the old world. PCR followed by ESI-MS is a novel, complex, but highly promising diagnostic methodology for the rapid assessment of rare or exotic pathogens, including intracellular bacteria.
AB - Bacterial pathogens not detectable via commercial blood culture assays represent an important challenge for infectious disease physicians, in particular if clinical symptoms of the illness are non-specific. In this report, Anaplasma phagocytophilum was detected directly in a peripheral blood sample from a febrile patient reporting a tick bite. This was done using a commercial system based on PCR followed by electrospray ionization mass spectrometry (ESI-MS). The diagnosis of a human granulocytic anaplasmosis infection was established using this diagnostic methodology for the first time. Human granulocytic anaplasmosis is a neglected zoonotic disease in Europe. Its seroprevalence is similar in North America and Europe, but in contrast to the USA, it is rarely diagnosed in the old world. PCR followed by ESI-MS is a novel, complex, but highly promising diagnostic methodology for the rapid assessment of rare or exotic pathogens, including intracellular bacteria.
KW - Adult
KW - Anaplasma phagocytophilum/genetics
KW - Animals
KW - Anti-Bacterial Agents/therapeutic use
KW - Doxycycline/therapeutic use
KW - Ehrlichiosis/blood
KW - Humans
KW - Male
KW - Neglected Diseases/diagnosis
KW - Polymerase Chain Reaction
KW - Spectrometry, Mass, Electrospray Ionization
KW - Tick Bites/complications
KW - Zoonoses/diagnosis
U2 - 10.1016/j.ijid.2017.05.006
DO - 10.1016/j.ijid.2017.05.006
M3 - SCORING: Journal article
C2 - 28526564
VL - 60
SP - 61
EP - 63
JO - INT J INFECT DIS
JF - INT J INFECT DIS
SN - 1201-9712
ER -
